Research Article

Effects of Acute L-Arginine Supplementation on the Performance of Recreationally Trained Crossfit® Males: A Randomized Double-Blind Placebo-Controlled Trial

Volume: 17 Number: 1 April 30, 2026
EN

Effects of Acute L-Arginine Supplementation on the Performance of Recreationally Trained Crossfit® Males: A Randomized Double-Blind Placebo-Controlled Trial

Abstract

The ergogenic effects of L-arginine endure throughout a range of exercise modalities; however, there is limited information proving its effectiveness during CrossFit® programs. The impacts of L-arginine supplementation on CrossFit® capability were investigated in this study. Sixteen recreationally trained CrossFit® men (22.5 ± 2.8 years, 1.8 ± 2.7 years of experience) participated in a double-blind, crossover study. All participants performed three sessions spaced one week apart to allow washout, supplemented with either L-arginine (dose A: 0.15 g/kg body mass; dose B: 6 g) or a placebo 60 minutes before exercise. The participants were instructed to perform "Cindy," which is a standardized CrossFit® benchmark workout consisting of the maximum number of rounds possible of 5 pull-ups, 10 push-ups, and 15 air squats. The total number of repetitions served as the performance metric. Additional measures included lactate, blood pressure, heart rate, and pulse oximetry pre- and post-exercise. ANOVA revealed no significant performance differences among conditions (dose A: 324.4 ± 108; dose B: 343.1 ± 117; placebo: 343.1 ± 100.4, p = 0.854). A meaningful learning influence was observed after the first session (315.0 ± 111.2 vs. 343.1 ± 94.2 repetitions, p = 0.0468) but not between the second and third sessions (p = 0.4837), without observable alteration in treatment order (p = 0.3333). Results indicated that supplementation with L-arginine did not boost performance during "Cindy" in recreationally trained CrossFit® men. Familiarization sessions and discovery of various CrossFit® workouts in trained athletes should be included in upcoming studies.

Keywords

Supporting Institution

This research was funded by the internal project of Universidad SEK (Sek-23001)

Ethical Statement

The present study was approved by the institutional ethics committee of Universidad SEK – Santiago, Chile (2023-19) and conducted in accordance with the Declaration of Helsinki. Participants were recruited between November - December 2023 through advertisements comparted by e-mail and paid visits to DUOC UC Institute, located at Maipú, Santiago, Chile. A total of 49 healthy men provided written informed consent to participate in the study.

Thanks

The authors would like to acknowledge the CrossFit® judges and assistants Alonso Jeria and Karin Kellinhusen during data collection, as well as Prof Hector Arriagada (Sports Nutritionist) and our fourth-year student for their unconditional support. The authors would like to thank the Cambridge University ReachSci Society for their support and assistance. This project was an outcome of the Mini-PhD Programme 2023: Food Science & Nutrition.

References

  1. Álvares, T. S., Meirelles, C. M., Bhambhani, Y. N., Paschoalin, V. M., & Gomes, P. S. (2011). L-Arginine as a potential ergogenic aid in healthy subjects. Sports Med, 41(3), 233-248. https://doi.org/10.2165/11538590-000000000-00000
  2. Baltazar-Martins, G., Brito de Souza, D., Aguilar-Navarro, M., Muñoz-Guerra, J., Plata, M. D. M., & Del Coso, J. (2019). Prevalence and patterns of dietary supplement use in elite Spanish athletes. J Int Soc Sports Nutr, 16(1), 30. https://doi.org/10.1186/s12970-019-0296-5
  3. Borg, G. A. (1982). Psychophysical bases of perceived exertion. Med Sci Sports Exerc, 14(5), 377-381.
  4. Butcher, S. J., Neyedly, T. J., Horvey, K. J., & Benko, C. R. (2015). Do physiological measures predict selected CrossFit(®) benchmark performance? Open Access J Sports Med, 6, 241-247. https://doi.org/10.2147/oajsm.S88265
  5. Claudino, J. G., Gabbett, T. J., Bourgeois, F., Souza, H. S., Miranda, R. C., Mezêncio, B., Soncin, R., Cardoso Filho, C. A., Bottaro, M., Hernandez, A. J., Amadio, A. C., & Serrão, J. C. (2018). CrossFit Overview: Systematic Review and Meta-analysis. Sports Med Open, 4(1), 11. https://doi.org/10.1186/s40798-018-0124-5
  6. Crawford, D. A., Drake, N. B., Carper, M. J., DeBlauw, J., & Heinrich, K. M. (2018). Are Changes in Physical Work Capacity Induced by High-Intensity Functional Training Related to Changes in Associated Physiologic Measures? Sports (Basel), 6(2). https://doi.org/10.3390/sports6020026
  7. Da Silva, V. L., Messias, F. R., Zanchi, N. E., Gerlinger-Romero, F., Duncan, M. J., & Guimarães-Ferreira, L. (2015). Effects of acute caffeine ingestion on resistance training performance and perceptual responses during repeated sets to failure. J Sports Med Phys Fitness, 55(5), 383-389.
  8. Fernandez-Fernandez, J., Sabido, R., Moya, D., Sarabia Marín, J. M., & Moya, M. (2015). Acute physiological responses during crossfit® workouts. European Journal of Human Movement, 35, 114-124.

Details

Primary Language

English

Subjects

Sports Nutrition

Journal Section

Research Article

Early Pub Date

April 23, 2026

Publication Date

April 30, 2026

Submission Date

May 12, 2025

Acceptance Date

February 24, 2026

Published in Issue

Year 2026 Volume: 17 Number: 1

APA
Belmar, J., Marmolejo, G., Valdés, D., águila, F., Klagges, C., Yarahmadi, M., Kimble, R., Zare, R., & Heinrich, K. M. (2026). Effects of Acute L-Arginine Supplementation on the Performance of Recreationally Trained Crossfit® Males: A Randomized Double-Blind Placebo-Controlled Trial. Pamukkale Journal of Sport Sciences, 17(1), 228-249. https://doi.org/10.54141/psbd.1686990
AMA
1.Belmar J, Marmolejo G, Valdés D, et al. Effects of Acute L-Arginine Supplementation on the Performance of Recreationally Trained Crossfit® Males: A Randomized Double-Blind Placebo-Controlled Trial. Pamukkale J Sport Sci. 2026;17(1):228-249. doi:10.54141/psbd.1686990
Chicago
Belmar, Joyce, Gheraldin Marmolejo, Diego Valdés, et al. 2026. “Effects of Acute L-Arginine Supplementation on the Performance of Recreationally Trained Crossfit® Males: A Randomized Double-Blind Placebo-Controlled Trial”. Pamukkale Journal of Sport Sciences 17 (1): 228-49. https://doi.org/10.54141/psbd.1686990.
EndNote
Belmar J, Marmolejo G, Valdés D, águila F, Klagges C, Yarahmadi M, Kimble R, Zare R, Heinrich KM (April 1, 2026) Effects of Acute L-Arginine Supplementation on the Performance of Recreationally Trained Crossfit® Males: A Randomized Double-Blind Placebo-Controlled Trial. Pamukkale Journal of Sport Sciences 17 1 228–249.
IEEE
[1]J. Belmar et al., “Effects of Acute L-Arginine Supplementation on the Performance of Recreationally Trained Crossfit® Males: A Randomized Double-Blind Placebo-Controlled Trial”, Pamukkale J Sport Sci, vol. 17, no. 1, pp. 228–249, Apr. 2026, doi: 10.54141/psbd.1686990.
ISNAD
Belmar, Joyce - Marmolejo, Gheraldin - Valdés, Diego - águila, Fabián - Klagges, Carolina - Yarahmadi, Mehdi - Kimble, Rachel - Zare, Reza - Heinrich, Katie M. “Effects of Acute L-Arginine Supplementation on the Performance of Recreationally Trained Crossfit® Males: A Randomized Double-Blind Placebo-Controlled Trial”. Pamukkale Journal of Sport Sciences 17/1 (April 1, 2026): 228-249. https://doi.org/10.54141/psbd.1686990.
JAMA
1.Belmar J, Marmolejo G, Valdés D, águila F, Klagges C, Yarahmadi M, Kimble R, Zare R, Heinrich KM. Effects of Acute L-Arginine Supplementation on the Performance of Recreationally Trained Crossfit® Males: A Randomized Double-Blind Placebo-Controlled Trial. Pamukkale J Sport Sci. 2026;17:228–249.
MLA
Belmar, Joyce, et al. “Effects of Acute L-Arginine Supplementation on the Performance of Recreationally Trained Crossfit® Males: A Randomized Double-Blind Placebo-Controlled Trial”. Pamukkale Journal of Sport Sciences, vol. 17, no. 1, Apr. 2026, pp. 228-49, doi:10.54141/psbd.1686990.
Vancouver
1.Joyce Belmar, Gheraldin Marmolejo, Diego Valdés, Fabián águila, Carolina Klagges, Mehdi Yarahmadi, Rachel Kimble, Reza Zare, Katie M. Heinrich. Effects of Acute L-Arginine Supplementation on the Performance of Recreationally Trained Crossfit® Males: A Randomized Double-Blind Placebo-Controlled Trial. Pamukkale J Sport Sci. 2026 Apr. 1;17(1):228-49. doi:10.54141/psbd.1686990