Microbial Analysis, Physicochemical Content and Insect Vectors Associated with Selected Dumpsites in Ijebu-Ode, Nigeria

Adewole Sebiomo

doi:10.71340/psi.1866014

EN TR

Microbial Analysis, Physicochemical Content and Insect Vectors Associated with Selected Dumpsites in Ijebu-Ode, Nigeria

Abstract

This study analyzed the types of insect vectors in dumpsite locations and the microorganisms associated with the insect vectors and dumpsites. The physicochemical and heavy metal content of the soil samples were also determined. Bacteria and fungi were isolated from the soils, housefly and cockroaches collected from the dumpsites. Atomic absorption spectrophotometer was used to determine the mineral content of the soil samples. Bacterial and fungal counts were also determined using the Quebec colony counter. Dehydrogenase, phosphatase, catalase, urease and peroxidase activities were estimated. Of the ten bacterial species identified Pseudomonas aeruginosa was the most prevalent isolate occurring at all the samples collected with a frequency of 20.3% while Streptococcal faecalis had the least percentage frequency of 3.1%. Seven fungal genera were identified. They include; Aspergillus niger, Trichoderma spp, Penicillium spp. The study showed a statistically significant (P<0.05) increase in the enzyme activities of the enzymes in the soil samples from the dumpsites compared to the control. Peroxidase and catalase activities of dumpsite soils were significantly higher (P≤0.05) than their control soil samples. There were significant differences (P≤0.05) in the mineral and heavy metal content of soil samples. The Na, Fe, Zn, Cu, Pd and Cd contents of the dump site soil samples were significantly higher than their control soil samples. However, the Ca, Mg, K contents of the control soil samples were significantly higher than those of the dump site soils. Fe had the highest value of 33.57±10.22 mg/kg compared to the other soil samples.

Keywords

insects, bacteria, fungi, viable counts

Nijerya’nın Ijebu-Ode Bölgesinde Seçilmiş Çöp Depolama Alanlarıyla İlişkili Mikrobiyal Analiz, Fizikokimyasal Bileşim ve Böcek Vektörleri

Abstract

Bu çalışmada, çöp depolama alanlarında bulunan böcek vektörlerinin türleri ile bu böcek vektörleri ve depolama alanlarıyla ilişkili mikroorganizmalar incelenmiştir. Ayrıca, toprak örneklerinin ağır metal ve fiziko-kimyasal içerikleri de belirlenmiştir. Çöp depolama alanlarından toplanan toprak, ev sineği ve hamamböceği örneklerinden bakteri ve mantarlar izole edilmiştir. Toprak örneklerinin mineral içeriklerinin belirlenmesinde atomik absorpsiyon spektrofotometresi kullanılmıştır. Bakteri ve mantar sayımları ise Quebec koloni sayacı kullanılarak gerçekleştirilmiştir. Dehidrogenaz, fosfataz, katalaz, üreaz ve peroksidaz enzim aktiviteleri değerlendirilmiştir. Tanımlanan on bakteri türü arasında, %20,3'lük görülme sıklığı ile Pseudomonas aeruginosa en yüksek orana sahip olurken, %3,1'lik görülme sıklığı ile Streptococcus faecalis en düşük orana sahip olmuştur. Yedi mantar cinsi tanımlanmıştır. Bunlar arasında Aspergillus niger, Trichoderma spp. ve Penicillium spp. yer almaktadır. Çalışmada incelenen çöp depolama alanı topraklarında, analizler sonrasında enzim aktivitelerinde anlamlı düzeyde artış (P≤0,05) gözlenmiştir. Depolama alanı topraklarının peroksidaz ve katalaz aktiviteleri, kontrol toprak örneklerine kıyasla daha yüksek bulunmuştur. Çöp depolama alanı topraklarında belirlenen Na, Fe, Zn, Cu, Pb ve Cd içerikleri, kontrol topraklarında elde edilen değerlerden daha yüksek bulunmuştur. Buna karşın, kontrol topraklarının Ca, Mg ve K içerikleri, depolama alanı topraklarına göre anlamlı derecede daha yüksek olarak belirlenmiştir. İncelenen elementler arasında Fe, 33,57 ± 10,22 mg/kg değeri ile en yüksek konsantrasyona sahip olmuştur.

Keywords

insects, bacteria, fungi, viable counts

References

[1] A. L. Al-Khatib, M. Maria, A. Salam, A. Zahra, H. Q. Shahen, and D. Kassinos, “Solid waste characterization, quantification, and management practices in developing countries,” J. Environ. Manage., vol. 91, no. 5, pp. 1131–1138, 2010, doi: 10.1016/j.jenvman.2010.01.003.
[2] F. I. Okoronkwo and G. C. Okpokwasili, “Open refuse dumpsites: effect on soil and underground water in Port Harcourt metropolis,” Int. J. Dev. Res., vol. 8, no. 4, pp. 19765–19775, 2018.
[3] B. O. Ojiego, S. A. Abdullahi, I. M. K. Gadzama, P. I. Bolorunduro, and A. Arowosebge, “Characterization of solid waste at Gosa dumpsite, Federal Capital Territory, Abuja,” Int. J. Sci. Res. Rev., vol. 8, no. 3, pp. 414–419, 2019.
[4] B. Onwuka and B. Mang, “Effects of soil temperature on some soil properties and plant growth,” Adv. Plants Agric. Res., vol. 8, no. 1, pp. 34‒37, 2018, doi: 10.15406/apar.2018.08.00288.
[5] M. S. Ayilara, O. S. Olanrewaju, O. O. Babalola, and O. Odeyan, “Waste Management through Composting: Challenges and Potentials,” Sustainability, vol. 12, no. 11, p. 4456, 2020, doi: 10.3390/su12114456.
[6] P. Alam and K. Ahmade, “Impact of Solid Waste on Health and The Environment,” Int. J. Sustain. Dev. Green Econ., vol. 2, pp. 165–168, 2013.
[7] A. T. Adewole, “Waste management towards sustainable development in Nigeria: A case study of Lagos State,” Int. NGO J., vol. 4, no. 4, pp. 173–179, 2009.
[8] E. Abba, M. Amina, Y. R. Lamogo, A. Jemimah, and K. P. Yoriyo, “Survey of Insect Vectors in Some Selected Dumpsites in Gombe Metropolis, Nigeria, Western Africa,” Asian J. Res. Zool., vol. 2, no. 3, pp. 1–9, 2019.
[9] G. Hoffmann and K. Teicher, “Colorimetric method for the determination of the urease activity in soil,” Zeit. Pflanzenernaehr. Dung. Bodenkunde., vol. 95, p. 55, 1961.
[10] H. Kumar, V. B. Singh, B. L. Meena, S. Gaur, and R. Singla, “Paraquat poisoning: a case report,” J. Clin. Diagn. Res., vol. 10, no. 10, 2016.

[11] R. G. Burns, “Enzyme activity in soil,” Soil Biol. Biochem., vol. 14, p. 425, 1982.
[12] C. Floch, E. Alarcon-Gutierrez, and S. Criquet, “ABTS assay of phenol oxidase activity in soil,” J. Microbiol. Methods, vol. 71, pp. 319–324, 2007.
[13] S. Riahi, A. B. Moghaddam, M. R. Ganjali, and P. Norouzi, “Determination of the oxidation potentials of pyrogallol and some of its derivatives: theory and experiment,” J. Theor. Comput. Chem., vol. 6, pp. 331–340, 2007. M. R. Roberge, “Methodology of enzymes determination and extraction,” in Soil Enzymes, Academic Press, 1978, pp. 341–373.
[14] R. L. Sinsabaugh, S. D. Allison, and P. G. Donovan, “Measuring phenol oxidase and peroxidase activities with pyrogallol, L-DOPA, and ABTS: Effect of assay conditions and soil type,” Soil Biol. Biochem., vol. 67, pp. 183–191, 2013.
[15] C. E. Bach, D. D. Warnock, D. J. Van Horn, and M. N. Weintraub, “Measuring phenol oxidase and peroxidase activities with pyrogallol,” Soil Biol. Biochem., 2013.
[16] Z. Stępniewska, A. Wolinska, and J. Ziomek, “Response of soil catalase activity to chromium contamination,” J. Environ. Sci., vol. 21, pp. 1142–1147, 2009.
[17] A. D. Vijayakumar, M. Subban, J. Jmbulingam, P. Annamalai, and P. T. Kalaichelvan, “A Rapid Sensitive Detection Method by Plate Assay for Catalase Activity from Bacterium Acinetobacter calcoaceticus AV6,” RRJMB, vol. 3, 2014.
[18] L. C. Blakemore, P. L. Searle, and B. K. Daly, Methods for chemical analysis of soils, Lower Hutt, New Zealand: New Zealand Soil Bureau, 1987, pp. 47–66.
[19] H. O. Obueh, N. Maduka, P. A. Egharevba-Ojo, K. E. Enerijiofi, and C. Ikhazuangbe-Benson, “Effect of dumpsite waste on soil, drinking water sources, and antibiotic susceptibility of isolates, in a sub-urban community, Benin city, Nigeria,” Sci. World J., vol. 19, no. 3, pp. 625–633, 2024.
[20] World Health Organization, Guidelines for drinking-water quality, incorporating 1st and 2nd Addenda, Vol. 1, Recommendations, 3rd ed. Geneva, Switzerland: WHO, 2010.
[21] J. A. Ibiam, A. N. Oko, C. C. Amaechi, C. O. Apie, U. I. Nwali, and H. E. Isu, “Effect of waste dumpsite on the surface and groundwater supplies using water quality index in Afikpo south local government, Ebonyi state,” World J. Adv. Res. Rev., vol. 21, no. 2, pp. 1343–1352, 2024, doi: 10.30574/wjarr.2024.21.2.0547.
[22] G. Bhalla, P. K. Swamee, A. Kumar, and A. Bansal, “Assessment of groundwater quality near municipal solid waste landfill by an aggregate index method,” Int. J. Environ. Sci., vol. 2, no. 2, pp. 1492–1503, 2012.
[23] N. N. Odu, A. L. Omunakwe, and M. Millicent, “Comparative assessment on the physicochemical water quality of wells and boreholes in two Rivers state communities, Nigeria,” Int. J. Res. Stud. Microbiol. Biotechnol., vol. 6, no. 3, pp. 5–20, 2020, doi: 10.20431/2454-9428.0603002.
[24] O. C. Nwiniyi, O. Uyi, E. J. Awosanya, I. Oyeyemi, A. M. Ugbenyen, A. Muhammad, O. A. Alabi, O. I. Ekwunife, C. O. Adetunji, and I. M. Omoruyi, “Review of drinking water quality in Nigeria: towards attaining sustainable development goal six,” Ann. Sci. Technol., vol. 5, no. 2, pp. 58–77, 2020.
[25] U. U. Udofia, U. U. Udiba, L. E. Udofia, N. N. Ezike, and S. U. Udiba, “Assessment of the impact of solid waste dumps on ground water quality, Calabar municipality, Nigeria,” J. Adv. Res. Pharm. Biol. Sci., vol. 2, no. 4, pp. 10–21, 2016, doi: 10.53555/nnpbs.v2i4.705.
[26] V. Samuel, W. O. M. Wilfred, V. N. Ogbonna, and V. O. Awah, “Survey of Microorganisms (Fungi) Associated with Dumpsites in Rumuolumeni, Port Harcourt, Rivers State,” J. Biol. Genet. Res., vol. 10, no. 1, pp. 78–87, 2024.
[27] “The History of Bergey's Manual,” in Bergey's Manual of Systematic Bacteriology, 1984, pp. 1–13. Positive Science International, 2(1), 2026, p. 27-42
[28] E. A. Ekundayo, B. G. Olubunmi, J. K. Kone, F. O. Ekundayo, D. T. Abayomi, and H. O. Adewoyin, “Bacteriological Assessment of Soil Samples Collected from Some Selected Dump Sites in Ondo Metropolis, Ondo State, Nigeria Mercedes,” F.U.T.A. J. Life Sci., vol. 5, no. 1, pp. 182–189, 2025.
[29] F. C. Nireti, A. M. Adetoun, and A. O. Abayomi, “Microbiological Assessment of Soil from Dumpsites in Oduduwa University Campus,” Int. J. Sci. Adv. Innov. Res., vol. 3, no. 1, pp. 25–29, 2018.
[30] E. O. Ohaeri, I. Callitus, N. Linus, and O. Emmanuel, “Physicochemical Properties of Soils from Dumpsite in the Federal University of Technology, Owerri, Nigeria,” Asian J. Adv. Res. Rep., vol. 18, no. 12, pp. 405–411, 2024.
[31] A. Taofeek, I. A. Alexander, S. O. Simon, O. T. Jimoh, F. Suleiman, U. M. Mohammed, A. U. Roseline, O. K. Lateef, J. A. Babatunde, and S. A. Adeola, “Impact of Dumpsite Wastes on Soil Properties and Heavy Metal Concentrations in Kontagora, Nigeria,” Glob. Sci. J., vol. 13, no. 8, pp. 29–65, 2025.

Details

Primary Language

English

Subjects

Analytical Biochemistry

Journal Section

Research Article

Authors

Adewole Sebiomo ^*
0000-0002-3930-0022
Nigeria

Publication Date

June 30, 2026

Submission Date

January 18, 2026

Acceptance Date

March 10, 2026

Published in Issue

Year 2026 Volume: 2 Number: 1

DOI

https://doi.org/10.71340/psi.1866014

IZ

https://izlik.org/JA56PM35DY

APA

Sebiomo, A. (2026). Microbial Analysis, Physicochemical Content and Insect Vectors Associated with Selected Dumpsites in Ijebu-Ode, Nigeria. Positive Science International, 2(1), 27-42. https://doi.org/10.71340/psi.1866014

AMA

1.Sebiomo A. Microbial Analysis, Physicochemical Content and Insect Vectors Associated with Selected Dumpsites in Ijebu-Ode, Nigeria. PSI. 2026;2(1):27-42. doi:10.71340/psi.1866014

Chicago

Sebiomo, Adewole. 2026. “Microbial Analysis, Physicochemical Content and Insect Vectors Associated With Selected Dumpsites in Ijebu-Ode, Nigeria”. Positive Science International 2 (1): 27-42. https://doi.org/10.71340/psi.1866014.

EndNote

Sebiomo A (June 1, 2026) Microbial Analysis, Physicochemical Content and Insect Vectors Associated with Selected Dumpsites in Ijebu-Ode, Nigeria. Positive Science International 2 1 27–42.

IEEE

[1]A. Sebiomo, “Microbial Analysis, Physicochemical Content and Insect Vectors Associated with Selected Dumpsites in Ijebu-Ode, Nigeria”, PSI, vol. 2, no. 1, pp. 27–42, June 2026, doi: 10.71340/psi.1866014.

ISNAD

Sebiomo, Adewole. “Microbial Analysis, Physicochemical Content and Insect Vectors Associated With Selected Dumpsites in Ijebu-Ode, Nigeria”. Positive Science International 2/1 (June 1, 2026): 27-42. https://doi.org/10.71340/psi.1866014.

JAMA

1.Sebiomo A. Microbial Analysis, Physicochemical Content and Insect Vectors Associated with Selected Dumpsites in Ijebu-Ode, Nigeria. PSI. 2026;2:27–42.

MLA

Sebiomo, Adewole. “Microbial Analysis, Physicochemical Content and Insect Vectors Associated With Selected Dumpsites in Ijebu-Ode, Nigeria”. Positive Science International, vol. 2, no. 1, June 2026, pp. 27-42, doi:10.71340/psi.1866014.

Vancouver

1.Adewole Sebiomo. Microbial Analysis, Physicochemical Content and Insect Vectors Associated with Selected Dumpsites in Ijebu-Ode, Nigeria. PSI. 2026 Jun. 1;2(1):27-42. doi:10.71340/psi.1866014

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Microbial Analysis, Physicochemical Content and Insect Vectors Associated with Selected Dumpsites in Ijebu-Ode, Nigeria

Abstract

Keywords

Nijerya’nın Ijebu-Ode Bölgesinde Seçilmiş Çöp Depolama Alanlarıyla İlişkili Mikrobiyal Analiz, Fizikokimyasal Bileşim ve Böcek Vektörleri

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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