Üzüm tanelerinde VvPDR genlerinin İfade Analizi

Year 2022, , 11 - 20, 30.06.2022

Birsen Çakır Aydemir , Selin Altıntaş

https://doi.org/10.53501/rteufemud.1081147

Abstract

ATP bağlayıcı kaset (ABC) taşıyıcı membran proteinleri, tüm organizmalarda büyük oranda korunmuş en büyük membran protein ailelerindendir. Ökaryotik ABC taşıyıcılarının yapısı, bir transmembran alanı (TMD) ve bir nükleotit bağlama alanı (NBD) olarak adlandırılan iki bölgeden oluşmaktadır. Bu proteinler, ökaryot canlılarda ABCA’dan ABCG’ye kadar devam eden toplam yedi ana aileye ayrılmaktadırlar ve bu ana aileler de kendi içlerinde alt ailelere ayrılmaktadır. Plazma zarında ve tonoplast, kloroplast, mitokondri ve peroksizomlar gibi organların zarlarında lokalizedirler ve çok sayıda işlevi yerine getirmektedirler. Başlangıçta detoksifikasyon işlemlerine katılan taşıyıcılar olarak tanımlanmış olmalarına rağmen daha sonraki yapılan çalışmalarda bu proteinlerin bitki büyümesi ve gelişim dönemlerinde ve çevresel streslere karşı tepkilerde de etkili oldukları gösterilmiştir. ABCG'nin tam molekül üyelerinden PDR alt grubu sadece mantar ve bitkilerde tanımlanmıştır. Plant pleiotropic drug resistance (PDR) alt ailesine ait proteinlerin çeşitli lipidlerin ve hormonların taşınmasında görev aldıkları ve aynı zamanda bu proteinlerin abiyotik ve biyotik streslere karşı tepkiler sırasında da görevlerinin olduğu yapılan farklı çalışmalarla bildirilmiştir. Yapılan bu çalışmada ise Vitis vinifera sp. genomunda tanımlanan ve PDR alt ailesine ait VvABCG35, VvABCG36, VvABCG37 genlerinin asmada meyve gelişim dönemleri boyunca gösterdiği ifadeler incelenmiş ve bu genlerin fonksiyonları hakkında fikir sahibi olmak için STRING veritabanı kullanılarak etkileşime girdiği diğer proteinler belirlenmiştir.

Keywords

Vitis vinifera L., ABC Proteinleri, ABCG, PDR, Real Time PCR

Supporting Institution

Ege Üniversitesi Bilimsel Araştırma Projeleri koordinatörlüğü tarafından desteklenmiştir.

Project Number

FYL-2018-20042

Thanks

Bu çalışma Yüksek Lisans projesi olarak Ege Üniversitesi Bilimsel Araştırma Projeleri koordinatörlüğü tarafından desteklenmiştir (FYL-2018-20042).

Expression Analysis of VvPDR Genes in Grape Berries

Abstract

The ATP-binding cassette (ABC) carrier membrane proteins are among the largest families of membrane proteins that are highly conserved in all organisms. The structure of eukaryotic ABC transporters consists of two domains called a transmembrane domain (TMD) and a nucleotide binding domain (NBD). In eukaryotes, it is classified into seven main families, ordered from ABCA to ABCG, and the main families are divided into subfamilies within themselves. They are localized in the membranes of a plant cell, such as the plasma membrane, tonoplast, chloroplast, mitochondria, and peroxisomes, and perform numerous functions. Initially identified as carriers involved in detoxification processes, they were later shown to be essential for organ growth, plant nutrition, plant growth, response to abiotic stress, pathogen resistance, and plant interaction with its environment. The plant pleiotropic drug resistance (PDR) subgroup consists of full structure members of ABCG subfamily that have only been identified in fungi and plants. It has been reported in various studies that the PDR subfamily is involved in the transport of various lipids and hormones, as well as playing a role in abiotic and biotic stresses. In this study, expressions of VvABCG35, VvABCG36, VvABCG37 genes belonging to the PDR subfamily, which are defined in Vitis vinifera sp. genome, during berry development periods were determined and other proteins with which they interact were examined by using the STRING database to have an idea about the functions of these genes.

Keywords

Vitis vinifera L., ABC Proteins, ABCG, PDR, Real-time PCR

Project Number

FYL-2018-20042

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