Kayın Mantarı (Pleurotus ostreatus) Genomunda ABC Proteinlerinden MDR Alt Ailesinin Biyoinformatik Analizi ve İfade Profili

Year 2021, Volume: 2 Issue: 2, 72 - 82, 31.12.2021

Hatice Kübra Düzel Birsen Çakır Aydemir

https://doi.org/10.53501/rteufemud.1017979

Cited By: 1

Abstract

Hücre zarında birçok farklı metabolik yolu kontrol eden yapılardan biri olan ve en büyük protein ailelerinden birini oluşturan ATP bağlayan kaset taşıyıcı proteinleri (ABC) ATP enerjisini kullanarak madde taşınımında görev alırlar. Çözünür ABC proteinleri transmembran taşınmasına dahil değildir, ancak hücresel süreçlerde, örneğin ribozom biyojenezi ve mRNA translasyonunda önemli bir rol oynamaktadır. ABC proteinlerinin bir alt ailesi olan çoklu ilaç direnci (MDR) alt ailesi, antimikrobiyal peptitler, lipit taşınması, feromon taşınması, mitokondriyal porfirin alımı, ökaryotik peptit taşınması, antijenlerin işlenmesi, oksidatif stresden korunma, ağır metal dayanıklılığı, v.b. olaylarda rol oynamaktadır. Bu çalışmada kayın mantarı (Pleurotus osteradus) genomunun biyoinformatik analizi ile ABC proteinlerinin bir alt üyesi olan ABCB gen ailesine ait 8 tane MDR protein kodlayan gen (PoMDRs) belirlenmiştir. PoMDRs genlerinin kodladığı proteinler ile filogenetik ağaç oluşturulmuştur. Ayrıca bu çalışma kapsamında ilk defa sap ve şapkada PoMDR genlerinin ifade seviyeleri incelenmiş ve bu genlerin ifade düzeylerinin benzer profillere sahip oldukları ancak bu genlerin ifade seviyelerinin şapkada daha fazla olduğu tespit edilmiştir.

Keywords

ABC Kaset Taşıyıcıları, MDR Alt Ailesi, mRNA İfadesi, Pleurotus Ostreatus, Kayın mantarı

Supporting Institution

Ege Üniversitesi Bilimsel araştırmalar proje koordinasyonluğu

Project Number

FYL-2018-20023

Thanks

Bu çalışma Kübra Düzel’in Yüksek Lisans tez çalışmasından elde edilmiştir. Kayın mantarı temini sağlayarak her türlü teknik desteği esirgemeyen Dr. Öğr. Üyesi Erkan EREN’e teşekkür ederiz. Bu çalışma Ege Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir. (Proje Numarası:FY-2018-20023)

Bioinformatic Analysis and Expression Profile of MDR Subfamily of ABC Proteins from Oyster Mushroom (Pleurotus ostreatus) Genome

Abstract

ATP-binding cassette proteins (ABC), one of the largest protein families involved in the transport of substances by consuming ATP in many different metabolic reactions in the cell membrane. Soluble ABC proteins, on the other hand, are not involved in transmembrane transport, but play an important role in cellular processes, such as ribosome biogenesis and mRNA translation. Multidrug resistance (MDR) subfamily, which is a subfamily of ABC proteins, plays a role in the transport of antimicrobial peptides, lipid transport, pheromone transport, mitochondrial porphyrin uptake, multidrug resistance, eukaryotic peptide transport, antigen processing, protection from oxidative stress, heavy metal resistance, etc. In this study, 8 MDR protein-coding genes (PoMDRs) belonging to the ABCB gene family, which is a sub-class of ABC proteins, were determined by bioinformatic analysis of the beech mushroom (Pleurotus osteradus) genome. And a phylogenetic tree was formed with the proteins encoded by these genes. In addition, within the scope of this study, the expression levels of PoMDR genes in the stem and cap were examined for the first time and it was determined that the expression levels of these genes had similar profiles, but the expression levels of these genes were higher in the cap.

Keywords

ABC cassette transporters, MDR subfamily, mRNA expression, Pleurotus ostreatus, Oyster mushroom

Project Number

FYL-2018-20023

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