Cimil Yaylası (Rize) Balının Melissopalinolojik ve Fiziko-Kimyasal Özellikleri

Year 2023, Volume: 4 Issue: 1, 51 - 64, 30.06.2023

Gülsüm Yetkin , Suzan Kundakçı , Esra Demir Kanbur , Kamil Coşkunçelebi , Serdar Makbul

https://doi.org/10.53501/rteufemud.1209594

Abstract

Bu çalışmada, Cimil Yaylası'nda üretilen balların melissopalinolojik ve fiziko-kimyasal özellikleri belirlenmiştir. Cimil Yaylası (Rize)’ndan farklı yükselti ve lokalitelerden 8 adet bal örneği alınmıştır. Tüm numuneler nem, şeker, prolin, diyastaz ve 5-Hidroksimetili furfural belirlemek için standart laboratuvar yöntemleriyle analiz edilmiştir. Ayrıca standart tekniklere göre melisopalinolojik analizler yapılmış ve bal örneklerinin polen kompozisyonları tespit edilmiştir. Melissopalinolojik çalışmalarda polenlerin baskın bir şekilde Asteraceae, Brassicaceae, Campanulaceae, Caprifoliaceae, Caryophyllaceae, Ericaceae, Fabaceae, Lamiaceae, Lauraceae, Onagraceae, Poaceae, Polygonaceae, Ranunculaceae ve Rosaceae familyalarına ait olduğu görülmüştür. Örneklerdeki nem miktarı 16.9-19.1, prolin miktarı ise 568-758 mg/kg olarak belirlenmiştir. Sonuçlarımız, incelenen bal örneklerinin polifloral ve monofloral olduğunu göstermiştir. Bu çalışma ile Cimil Yaylası balları ilk kez melissopalinolojik ve fiziko-kimyasal özellikleri bakımından detaylı olarak incelenmiştir. Balın melissopalinolojik ve fiziko-kimyasal analiz sonuçlarının Avrupa Birliği Standartları ve Türk Gıda Kodeksi Bal Tebliği'ne uygun olduğu görülmüştür.

Keywords

Cimil Yaylası, Bal, Melissopalinoloji, Rize

Project Number

1919B012003596

Melissopalynological and Physico-Chemical Properties of Cimil Plateau (Rize) Honey

Abstract

In this study, melissopalynological and physico-chemical properties of the honey produced in the Cimil Plateau were determined. Eight honey samples were taken from Cimil Plateau (Rize province) from different altitudes and localities. All samples were analysed by standard laboratory methods to determine moisture, sugar, proline, diastase and 5-Hydroxymethyl furfural (HMF) parameters. Also, melissopalynological analyses were made according to the standard techniques and determined pollen composition of the honey samples. Melissopalynological studies showed that the pollens dominantly belong to Asteraceae, Brassicaceae, Campanulaceae, Caprifoliaceae, Caryophyllaceae, Ericaceae, Fabaceae, Lamiaceae, Lauraceae, Onagraceae, Poaceae, Polygonaceae, Ranunculaceae and Rosaceae families. The moisture content of the samples was determined to be 16.9-19.1 %, the proline content was 568-758 mg/kg. Our results showed that the examined honey samples are polyfloral and monofloral. This is the first report performed on Cimil honey based on melissopalynological and physico-chemical characteristics. It has been observed that the melissopalynological and physico-chemical analysis results of honey are following European Union Standards and Turkish Food Codex Honey Communiqué.

Keywords

Cimil Plateau, Honey, Melissopalynology, Rize

Supporting Institution

TUBITAK

Project Number

1919B012003596

References

• Akman, Y. (1995). Turkey forest vegetation. Ankara University of Science Faculty Publishing, 450.
• Anşin, R. (1981). Main vegetation types of the inner and coastal part of the East Black Sea Region. Journal of the Foresty Faculty of the Karadeniz Technical University, 4(1), 14-25.
• Anonymous. (2009c). Requirements for the transit of honey bees (Apis mellifera) through the United States.
• Anonymous. (2020). Turkish Food Codex Honey Communiqué. Communiqué No: 2020/7.
• Batu, A., Küçük, E., Çimen, M. (2013). Determination of the physicochemical and biochemical values of flower honeys obtained from eastern Anatolia and Eastern Black Sea Regions. Electronic Journal of Food Technologies, 8(1), 52-62.
• Baykal, H., Atamov, V., Yüksek, T. (2018). Flora of Tunca Valley Natural Park and environs (Ardeşen-Rize/Turkey). Biological Diversity and Conservation, 11(3), 9-23.
• Baykal, H. (2019). Flora of Akyamaç Waterfall natural park and environs (Rize/Turkey). Biological Diversity and Conservation, 12(1), 128-137. https://doi.org/10.5505/biodicon.2019.98608.
• Bayram, N. E., Demir, E. (2018). Specifying some quality characteristics of monofloral and multifloral honey samples. Hacettepe Journal of Biology and Chemistry, 46(3), 417-423. https://doi.org/10.15671/HJBC.2018.249.
• Bogdanov, S., Martin, P., Lüllmann, C. (1997). Harmonised methods of the European honey commission. Apidologie, (France).
• Bogdanov, S., Ruoff, K., Oddo, L. P. (2004). Physico-Chemical methods for the characterisation of unifloral honeys: A Review. Apidologie, 35(1), 4-17. https://doi.org/10.1051/apido: 2004047.
• Çelemli, O. G., Özenirler, C., Bayram, N. E., Zare, G., Sorkun, K. (2018). Melissopalynological analysis for geographical marking of Kars Honey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 24(1), 53-59. https://doi.org/10.9775/kvfd.2017.18262
• Çelemli, O. G., Özkök, A., Özenirler, Ç., Mayda, N., Zare, G., Sorkun, K. (2021). Highlighting The Melissopalynological and Physicochemical Characteristics of Ayder-Rize (Turkey). Communications Faculty of Sciences University of Ankara Series C Biology, 30(2), 119-133. https://doi.org/ 10.53447/communc.906788.
• Çobanoğlu, M. (2012). Flora and vegetation of area between Güneysu-Çağrankaya. Master Thesis. Recep Tayyip Erdogan University, Graduate School of Natural and Applied Sciences, Rize, Türkiye.
• Demir, E. (2011). Pollen analysis of honey samples collected from Komati (Çamlıhemşin) Plateau. Mellifera, 12-24, 11-16.
• Demir, E. (2013). Flora of Ayder-Çeymakçur Plateaus and chemical and palynological properties of local honey. Master Thesis. Recep Tayyip Erdogan University, Graduate School of Natural and Applied Sciences, Rize, Türkiye.
• Demir, E., Atamov, V. (2019). The Flora of the area between Galerduzu-Çeymakçur Plateau (Camlıhemsin/Rize). Journal of Anatolian Environmental and Animal Sciences, 4(2), 201-210. https://doi.org/10.35229/jaes.568972.
• Derebaşı, E., Bulut, G., Col, M., Güney, F., Yaşar, N., Ertürk, Ö. (2014). Physicochemical and residue analysis of honey from Black Sea region of Turkey. Fresenius Environmental Bulletin, 23(1), 10-17.
• Devillers, J., Morlot, M., Pham-Delegue, M. H., Dore, J.C. (2004). Classification of monofloral honeys based on their quality control data. Food chemistry, 86(2), 305-312. https://doi.org/10.1016/j.foodchem.2003.09.029
• Erdoğan, N., Pehlivan, S., Doğan, C. (2009). Pollen analysis of honeys from Sapanca-Karapürçek-Geyve and Taraklı districts of Adapazarı province (Turkey). Mellifera, 9, 17-18.
• Güner, A., Vural, M., Sorkun, K. (1987). Rize Flora, Vegetation and Pollen Analysis of Local Honey, TÜBİTAK, TBAG-650, Ankara.
• Hotaman, H. E. (2015). Investigation of some bioactivite properties of Anzer honey and pollen in vitro. Master Thesis. Recep Tayyip Erdogan University, Graduate School of Natural and Applied Sciences, Rize, Türkiye.
• IHC (2009). International Honey Comission. 2009.
• Islam, A., Khalil, I., Islam, N., Moniruzzaman, M., Mottalib, A., Sulaiman, S. A., Gan, S.H. (2012). Physicochemical and antioxidant properties of Bangladeshi honeys stored for more than one year. BMC Complementary and Alternative Medicine, 12(1), 177. https://doi.org/10.1186/1472-6882-12-177
• Kanbur, E. D., Yüksek, T., Atamov, V., Özcelik, A. E. (2021). A comparison of the physicochemical properties of chestnut and highland honey: The case of Senoz Valley in the Rize province of Turkey. Food Chemistry, 345, 128864. https://doi.org/10.1016/j.foodchem.2020.128864.
• Kanbur, E. D., Atamov, V. (2022). Chemical and palynological properties of Ayder (Camlihemsin/Rize) Honeys. Journal of Apitherapy and Nature, 5(2), 119-132. https://doi.org/10.35206/jan.1120082.
• Kaya, Z., Binzet, R., Orcan, N. (2005). Pollen analyses of honeys from some regions in Turkey. Apiacta, 40, 10-15.
• Kurtagic, H., Memic, M., Barudanovic, S. (2016). Determination of type of honey produced in the different climatic regions of Bosnia and Herzegovina. International journal of environmental science and technology. 13, 2721-2730. https://doi.org/10.1007/s13762-016-1101-5.
• Louveaux, J., Maurizio, A., Vorwohl, G. (1970). U.S. Methods of melissopalynology. Bee World, 51: 125-138; (1978 reoubl. ed.) 59: 139-157.
• Malkoç, M., Çakır, H., Yakup, K, Zehra, C., Kolaylı, S. (2019). Phenolic composition and antioxidant properties of Anzer honey from black sea region of Turkey. Uludağ Arıcılık Dergisi, 19(2), 143-151. https://doi.org/10.31467/uluaricilik.602906.
• Meda, A., Lamien, C. E., Millogo, J., Romito, M., Nacoulma, O. G. (2005). Physiochemical analyses of Burkina Fasan honey. Acta Veterinaria Brno, 74 (1), 147-152. https://doi.org/10.1016/j.foodchem.2004.10.006.
• Moniruzzaman, M., Rodríguez, I., Ramil, M., Cela, R., Sulaiman, S., Gan, S. (2014). Assessment of gas chromatography time-of-flight accurate mass spectrometry for identification of volatile and semi-volatile compounds in honey. Talant, 129, 505- 515. https://doi.org/10.1016/j.talanta.2014.06.019.
• Ough., C. S. (1960). Rapid determination of proline in grapes and wines. Journal Food Science, 34, 228-230. https://doi.org/10.1111/j.1365-2621.1969.tb10327.x.
• Özbalcı, B., Boyacı, I. H., Topcu, A., Kadılar, C., Tamer, U. (2013). Rapid analysis of sugar in honey by processing Raman spectrum using chemometric methods and artificial neural Networks. Food Chemistry, 136, 1444-1452. https://doi.org/10.1016/j.foodchem.2012.09.064.
• Özgüven, M., Demircan, E., Özçelik, B. (2020). Determination of physicochemical properties of flower honeys produced in various locations and their compliance with the Turkish food codex. European Journal of Science and Technology, 20, 321-326. https://doi.org/10.31590/ejosat.758399.
• Özler, H. (2018). Pollen analysis of the honey from South Anatolia. Uludağ Arıcılık Dergisi, 18(2), 73-86. https://doi.org//10.31467/uluaricilik.485004.
• Özmen, N., Alkın, E. (2006). The antimicrobial features of honey and the effects on human health. Uludağ Bee Journal, 6(4), 155-160.
• Polat, R., Esim, N., Urusan, Z., Caf, A., Ahıskalı, M. (2020). An evaluation on Solhan (Bingöl/Turkey) flora with regard to beekeeping. Turkish Journal of Nature and Science, 9, 1-10. https://doi.org/10.46810/tdfd.772388.
• Saygılı, R. (2020). Geography map. com. Rize province map. https://tr.pinterest.com/pin/629941066623413385.
• Saral, Ö. (2023). An investigation into chestnut honeys from Artvin province in Turkiye: their physicochemical properties, phenolic profiles and antioxidant activities. Chemistry & Biodiversity, 1-7. https://doi.org/10.1002/cbdv.202201162.
• Spilioti, E., Jaakkola, M., Tolonen, T., Lipponen, M., Virtanen, V., Chinou, I., Moutsatsou, P. (2014). Phenolic acid composition, antiatherogenic and anticancer potential of honeys derived from various regions in Greece. Plos One, 9(4). https://doi.org/10.1371/journal.pone.0094860.
• Sorkun, K., İnceoğlu, Ö. (1984). Dominant pollens in honey of the Central Anatolian region. Natural Science Journal, 8(3), 377-381.
• Sorkun, K., Güner, A., Vural, M. (1989). Pollen analysis in Rize honey. Nature Turkish Journal Botanical, 13(3), 547-554.
• Sorkun, K., Doğan, C. (1995). Pollen analysis of Rize-Anzer (Turkish) honey. Apiacta/An International Technical Magazine of Apicultural and Economic Information, 3, 75-81.
• Taşkın, D., İnce, A. (2009). Pollen Analysis of Burdur Region Honeys. Journal of Natural & Applied Sciences 13 (1), 10-19.
• Temizer, İ. K., Güder, A., Çelemli, Ö. G. (2016). Botanic origin, various physicochemical and antioxidant properties of honey samples from Giresun, Turkey. Hacettepe Journal of Biology and Chemistry, 44(3), 209-215. https://doi.org/10.15671/HJBC.20164420563.
• Terzioğlu, S. (1998). Flora and vegetation of Uzungöl (Trabzon-Çaykara) and its environment. Doctora Thesis. Karadeniz Technical University, Institute of Science, Trabzon, Türkiye.
• Tosi, E., Ciappini, M., Re, E., Lucero, H. (2002). Honey thermal treatment effects on hydroxmethylfurfural content. Food Chemistry, 77, 71-74. https://doi.org/10.1016/S0308-8146 (01)00325-9.
• Tosunoğlu, H., Tosunoğlu, A., Ergün, N., Bicakci, A. (2022). Botanical characterisation of natural honey samples from a high altitudinal region, Gümüşhane, east-Türkiye. Grana, 62(1), 1-11. https://doi.org/10.1080/00173134.2022.2138532
• TÜIK (Turkish Statistical Institute), 2017. URL: https://arastirma.tarimorman.gov.tr/aricilik/Belgeler/istatistik/T%C3%BCrkiye%20Ar%C4%B1c%C4%B1l%C4%B1k%20%C4%B0statistikleri%20(TU%C4%B0K,%202017).pdf Accepted 09.03.2020.
• Tunçel, H. (1992). Beekeeping in Turkey (1966-1986). Journal of Turkish Geography Application and Research Center, 118, 20-25. https://doi.org//10.1080/0005772X.2005.11417326.
• Ulusoy, E. (2010). Determination of phenolic components of Anzer honey and pollen by high performance liquid chromatography and their antioxidant properties. Doctora Thesis. Karadeniz Technical University, Graduate School of Health Sciences, Trabzon, Türkiye.
• Ünal, A., Sağlam, C. (2009). Flora in the region between Ayrancı Dam, Karakükürtlü Mountain, Alahan, and Karaman regions. Dumlupınar Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 17, 27-47.
• Yurtsever, N., Sorkun, K. (2005). Determination of Botanical Origin of the honey produced in the Kemaliye-Erzincan Region in Eastern Turkey by Microscopical and Organoleptical Analyses. Mellifera, 5(9), 12-13.
• Weston, R. J., Brocklebank, L. K. (1999). The oligosaccharide composition of some New Zealand honeys. Food Chemistry, 64, 33-37. https://doi.org/10.1016/S0308-8146(98)00099-5.
• White, J. W. (1994). The role of HMF and diastase assays in honey quality evaluation. Bee world, 75 (3), 104-117. https://doi.org/10.1080/0005772X.1994.11099213