Abstract
Objective: Laryngeal malignancy known as the most frequently diagnosed cancer between head and neck tumor, covering 2.4% of all tumors and is the
11th most occurrence and deadliest tumor in the world. Research has shown that microRNAs (miRNAs) play a considerable role in the development of
laryngeal malignancy. Moreover, studies also focus on the relationship between clinical relevance and miRNA deregulation in cancer illness. According to
recent evidence, miR-205, miR-126, miR-302, and miR-132 have possible roles in the carcinogenesis process of the LSCC. Therefore, in this research, we
aimed to investigate whether there are any considerable changes in the microRNAs expression level and also if they have any remarkable potential as a
prognostic or diagnostic biomarker for laryngeal squamous cell carcinoma (LSCC).
Methods: Thirty LSCC patients were included in this research, and tumor tissues and healthy normal tissues were collected during laryngectomy. Total RNA
extraction from the normal and cancer tissues was accomplished and after the quality control, cDNA was synthesized, and finally, target miRNAs expression
was determined by qPCR. The expression level of genes and their relationship with the patient’s clinicopathological characteristics were analyzed using
appropriate statistical tests.
Results: According to the study results, miR-302 (fold change: 1.o5, P-value = 0.0001) and miR-132 (fold change: 1.28, P-value = 0.0001), is considerably
overexpressed in cancer tissues compare to normal peripheral tissues. We also found that miR-126 (fold change: 0.251, P-value = 0.0001) and miR-205
(fold change: 0.241, P-value<0.0001) is down-regulated in LSCC tumor tissue. Among these microRNAs, only mir-205 expression had no association with
clinical-pathological features in tumor tissue.
Conclusion: The results of this research revealed the potential of the miR-302, miR-132, miR-126, and miR-205 as prognostic or diagnostic biomarkers in
LSCC.
Keywords
References
- Grozescu T, Popa F. Prostate cancer between prognosis and adequate/proper therapy. J Med Life. 2017;10(1):5-12
Abstract
Objective: Laryngeal malignancy known as the most frequently diagnosed cancer between head and neck tumor, covering 2.4% of all tumors and is the 11th most occurrence and deadliest tumor in the world. Research has shown that microRNAs (miRNAs) play a considerable role in the development of laryngeal malignancy. Moreover, studies also focus on the relationship between clinical relevance and miRNA deregulation in cancer illness. According to recent evidence, miR-205, miR-126, miR-302, and miR-132 have possible roles in the carcinogenesis process of the LSCC. Therefore, in this research, we aimed to investigate whether there are any considerable changes in the microRNAs expression level and also if they have any remarkable potential as a prognostic or diagnostic biomarker for laryngeal squamous cell carcinoma (LSCC).
Methods: Thirty LSCC patients were included in this research, and tumor tissues and healthy normal tissues were collected during laryngectomy. Total RNA extraction from the normal and cancer tissues was accomplished and after the quality control, cDNA was synthesized, and finally, target miRNAs expression was determined by qPCR. The expression level of genes and their relationship with the patient’s clinicopathological characteristics were analyzed using appropriate statistical tests.
Results: According to the study results, miR-302 (fold change: 1.o5, P-value = 0.0001) and miR-132 (fold change: 1.28, P-value = 0.0001), is considerably overexpressed in cancer tissues compare to normal peripheral tissues. We also found that miR-126 (fold change: 0.251, P-value = 0.0001) and miR-205 (fold change: 0.241, P-value<0.0001) is down-regulated in LSCC tumor tissue. Among these microRNAs, only mir-205 expression had no association with clinical-pathological features in tumor tissue.
Conclusion: The results of this research revealed the potential of the miR-302, miR-132, miR-126, and miR-205 as prognostic or diagnostic biomarkers in LSCC.
Keywords
References
- Grozescu T, Popa F. Prostate cancer between prognosis and adequate/proper therapy. J Med Life. 2017;10(1):5-12
Details
| Primary Language | English |
|---|---|
| Subjects | Clinical Sciences |
| Journal Section | Meeting Abstract |
| Authors | |
| Publication Date | August 9, 2022 |
| Submission Date | July 18, 2022 |
| Published in Issue | Year 2022 |