INVESTIGATION OF THE EFFECT PISTACIA VERA FROM DIFFERENT REGIONS ON OXIDATIVE STRESS AND PROLIFERATION IN BREAST CANCER CELL LINE
Abstract
Objective: To investigate the effectiveness of extracts obtained from the inner shell of P. vera plants collected from different regions
Materials and Methods: Flavanol and methanol extracts were prepared from the inner shell of P. vera. The cytotoxic effects of the prepared extracts on
invasive breast cancer cell lines were determined by our modified MTT cytotoxicity test. Total antioxidant and total oxidant tests were applied, and
oxidative stress indices were examined. Migration test was used to investigate the effects of the agents used on the motility of the cells, and colony
formation assay was performed to investigate the effect on the colony forming feature.
Results: P. vera methanol extracts were found to be more cytotoxic than flavanol extracts. Siirt P. vera Methanol extract was found to be the most cytotoxic
extract. P. vera Urfa dissolved in methanol extract due to has high oxidative stress was the most effective in reducing migration in MDA-MB-231 cell line
among the groups.
Conclusions: Urfa P. vera Methanol extract is most effective in invasive breast cancer cells due to high oxidative stress level that occurs.
Keywords
References
- Arayıcı ME YF, Ellidokuz H. Importance of Cancer Registration and its Place in Cancer Control. Sted. 2021;30(5):366-75.
INVESTIGATION OF THE EFFECT PISTACIA VERA FROM DIFFERENT REGIONS ON OXIDATIVE STRESS AND PROLIFERATION IN BREAST CANCER CELL LINE
Abstract
Objective: To investigate the effectiveness of extracts obtained from the inner shell of P. vera plants collected from different regions
Materials and Methods: Flavanol and methanol extracts were prepared from the inner shell of P. vera. The cytotoxic effects of the prepared extracts on
invasive breast cancer cell lines were determined by our modified MTT cytotoxicity test. Total antioxidant and total oxidant tests were applied, and
oxidative stress indices were examined. Migration test was used to investigate the effects of the agents used on the motility of the cells, and colony
formation assay was performed to investigate the effect on the colony forming feature.
Results: P. vera methanol extracts were found to be more cytotoxic than flavanol extracts. Siirt P. vera Methanol extract was found to be the most cytotoxic
extract. P. vera Urfa dissolved in methanol extract due to has high oxidative stress was the most effective in reducing migration in MDA-MB-231 cell line
among the groups.
Conclusions: Urfa P. vera Methanol extract is most effective in invasive breast cancer cells due to high oxidative stress level that occurs.
Keywords
References
- Arayıcı ME YF, Ellidokuz H. Importance of Cancer Registration and its Place in Cancer Control. Sted. 2021;30(5):366-75.
Details
| Primary Language | English |
|---|---|
| Subjects | Clinical Sciences |
| Journal Section | Meeting Abstract |
| Authors | |
| Publication Date | August 9, 2022 |
| Submission Date | July 19, 2022 |
| Published in Issue | Year 2022 |