Detection of SARS-COV-2 Virus Causing COVID-19 Disease by Real-Time Polymerase Chain Reaction

Abstract

In December 2019, an undetermined cause of pneumonia was reported in the city of Wuhan in China, and then this disease was named COVID-19. In March 2020, COVID-19 disease was declared as a pandemic by the World Health Organization (WHO). It has been stated by WHO that the polymerase chain reaction technique may be applied for diagnosis in asymptomatic or mild symptomic cases. In our country, RT-PCR applications are carried out in accordance with the WHO guideline and approved by the Ministry of Health in order to make a exact diagnosis of SARS-CoV2. This review summarizes the process of detecting SARS-COV-2 virus by RT-PCR, which causes COVID-19 disease.

Keywords

• COVID-19,polymerase chain reaction,virus detection

COVID-19 Hastalığına Neden Olan SARS-COV-2 Virüsünün Gerçek Zamanlı Polimeraz Zincir Reaksiyonu ile Belirlenmesi

Abstract

2019 yılının Aralık ayında nedeni belirlenememiş bir pnömoni vakası Çin’de Wuhan şehrinde bildirilmiştir ve ardından bu hastalık COVID-19 olarak isimlendirilmiştir. 2020 yılının Mart ayında Dünya Sağlık Örgütü (DSÖ) tarafından COVID-19 hastalığı pandemi olarak ilan edilmiştir. DSÖ tarafından asemptomatik ya da hafif semptomlu vakalarda polimeraz zincir reaksiyonu (PCR) tekniğinin tanı koyma amacıyla uygulanabileceği belirtilmiştir. Ülkemizde, SARS-CoV2 kesin tanısının konması amacıyla gerçek zamanlı PCR (RT-PCR) uygulamaları DSÖ’nün kılavuzunda belirttiği ve Sağlık Bakanlığı’nın onayladığı şekilde gerçekleştirilmektedir. Bu derlemede, COVID-19 hastalığına neden olan SARS-COV-2 virüsünün RT-PCR ile tespitine ilişkin süreç özetlenmektedir.

Keywords

• COVID-19,polimeraz zincir reaksiyonu,virüs tespiti

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