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B HÜCRELİ NON-HODGKİN LENFOMADA DICER1 VE BAFF GEN MUTASYONLARININ ARAŞTIRILMASI

Year 2024, Volume: 7 Issue: 1, 8 - 16, 15.02.2024
https://doi.org/10.26650/JARHS2023-1344811

Abstract

Amaç: DICER1 ve BAFF geni mutasyonlarının T hücreli lenfoma progresyonunda etkili olduğu bilinmektedir. Bu durum B hücreli lenfomaların progresyonunda da DICER1 ve BAFF genlerinin rolünün olabileceğini düşündürmüştür. DICER1 ve BAFF geninin ekspresyon ve mutasyonlarını araştıran birçok çalışma bulunmasına rağmen, B hücreli non-Hodgkin lenfoma progresyonunda tümör baskılayıcı etkisinin nasıl oluştuğu ile ilgili olarak yeterli bilgi bulunmamaktadır. Bu sebeple çalışmada, DICER1 ve BAFF genlerinin B-NHL gelişimindeki rolünün ne olduğunun belirlenmesi amaçlanmıştır.

Gereç ve Yöntem: Çalışmaya 1991-1997 yılları arasında İstanbul Üniversitesi, Onkoloji Enstitüsü, Klinik Onkoloji Anabilim Dalı’na başvurmuş ve B-NHL tanısı almış 60 hastaya ait DNA örnekleri dâhil edilmiştir. Kontrol grubu olarak hastalarla yaş, cinsiyet ve ırk olarak eşleştirilmiş 30 sağlıklı kişinin lenfositlerinden elde edilen DNA materyalleri kullanılmıştır. DICER1 geninde yer alan c.+3473A>G(rs3742330) polimorfizmi ile BAFF genindeki c.-871C>T(rs9514828) tek nükleotid polimorfizmi PCR-RFLP yöntemiyle incelenmiştir. Ayrıca DICER1 geninin 11. ve 25. ekzonları mutasyon varlığı açısından SANGER dizi analizi yöntemiyle değerlendirilmiştir. Kontrol ve hasta grubunun sonuçları polimorfizm ve mutasyon varlığı açısından Ki-kare ve Fisher testleriyle analiz edilmiştir.

Bulgular: DICER1 genindeki c.+3473A>G(rs3742330) ile BAFF genindeki c.-871C>T(rs 9514828) polimorfik bölgeleri hasta ve kontrol grubunda incelenmiş ancak istatistiksel olarak anlamlı bir ilişki bulunamamıştır. DICER1 geninin 11. ve 25. Ekzonları araştırıldığında ise yine hasta ve kontrol grubu arasında istatiksel olarak anlamlı bir ilişki bulunamamıştır (p>0,05).

Sonuç: Hasta ve kontrol grupları arasında bir farkın bulunmaması, B-NHL oluşumunda farklı genetik mekanizmaların etkisinin olabileceğini düşündürmektedir. Çalışmadaki popülasyon sayısının az olması, sonuçlar arasında anlamlı bir fark bulunamamasının nedenlerinden biridir. Daha geniş hasta ve kontrol grubunda ek çalışmalara ihtiyaç vardır.

Project Number

43912

References

  • Stratton MR. Journeys into the genome of cancer cells. EMBO Mol Med 2013;5(2):169-72. google scholar
  • Thandra KC, Barsouk A, Saginala K, Padala SA, Barsouk A, Rawla P. Epidemiology of non-hodgkin’s lymphoma. Med Sci 2021;9(1):5- 14. google scholar
  • Erratum. Global cancer statistics 2018: Globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 2020;70(4):313. google scholar
  • Yildirim M, Karakilinc H, Yildiz M, Kurtoglu E, Dilli UD, Goktas S, et al. Non-hodgkin lymphoma and pesticide exposure in Turkey. Asian Pac J Cancer Prev 2013;14(6):3461-3. google scholar
  • Armitage JO, Weisenburger DD. New approach to classifying nonhodgkin’s lymphomas: clinical features of the major histologic subtypes. Non-hodgkin’s lymphoma classification project. J Clin Oncol 1998;16(8):2780-95. google scholar
  • Armitage JO, Gascoyne RD, Lunning MA, Cavalli F. Non-hodgkin lymphoma. Lancet 2017;390(10091):298-310. google scholar
  • Foulkes WD, Priest JR, Duchaine TF. DICER1: mutations, microRNAs and mechanisms. Nat Rev Cancer 2014;14(10):662-72. google scholar
  • Li X, Tian X, Zhang B, Zhang Y, Chen J. Variation in dicer gene is associated with increased survival in T-cell lymphoma. PLoS One 2012;7(12):e51640. google scholar
  • Di Lisio L, Martinez N, Montes-Moreno S, Piris-Villaespesa M, Sanchez-Beato M, Piris MA. The role of miRNAs in the pathogenesis and diagnosis of B-cell lymphomas. Blood 2012;120(9):1782-90. google scholar
  • Li X, Tian X, Zhang B, Chen J. Polymorphisms in microRNArelated genes are associated with survival of patients with T-cell lymphoma. Oncologist 2014;19(3):243-9. google scholar
  • Murray MJ, Bailey S, Raby KL, Saini HK, de Kock L, Burke GA, et al. Serum levels of mature microRNAs in DICER1-mutated pleuropulmonary blastoma. Oncogenesis 2014;3(2):e87. google scholar
  • Koizumi M, Hiasa Y, Kumagi T, Yamanishi H, Azemoto N, Kobata T, et al. Increased B cell-activating factor promotes tumor invasion and metastasis in human pancreatic cancer. PLoS One 2013;8(8):e71367. google scholar
  • Naradikian MS, Perate AR, Cancro MP. BAFF receptors and ligands create independent homeostatic niches for B cell subsets. Curr Opin Immunol 2015;34:126-9. google scholar
  • Yang S, Li JY, Xu W. Role of BAFF/BAFF-R axis in B-cell non-hodgkin lymphoma. Crit Rev Oncol Hematol 2014;91(2):113-22. google scholar
  • Li YJ, Jiang WQ, Rao HL, Huang JJ, Xia Y, Huang HQ, et al. Expression of BAFF and BAFF-R in follicular lymphoma: correlation with clinicopathologic characteristics and survival outcomes. PLoS One 2012;7(12):e50936. google scholar
  • Novak AJ, Grote DM, Stenson M, Ziesmer SC, Witzig TE, Habermann TM, et al. Expression of BLyS and its receptors in B-cell non-hodgkin lymphoma: correlation with disease activity and patient outcome. Blood 2004;104(8):2247-53. google scholar
  • Zhai K, Tian X, Wu C, Lu N, Chang J, Huang L, et al. Cytokine BAFF gene variation is associated with survival of patients with T-cell lymphomas. Clin Cancer Res 2012;18(8):2250-6. google scholar

INVESTIGATION OF DICER1 AND BAFF GENE MUTATIONS IN B-CELL NON-HODGKIN LYMPHOMA

Year 2024, Volume: 7 Issue: 1, 8 - 16, 15.02.2024
https://doi.org/10.26650/JARHS2023-1344811

Abstract

Objective: DICER1 and BAFF gene mutations are effective in T-cell lymphoma progression. Therefore, DICER1 and BAFF genes may have a role in the progression of B-cell lymphomas. For this reason, it was aimed to determine the role of DICER1 and BAFF genes in the development of B-NHL.

Materials and Methods: The study included DNA samples from 60 patients diagnosed with B-NHL who had applied to the Istanbul University,Institute of Oncology, Department of Clinical Oncology between 1991 and 1997. DNA materials obtained from lymphocytes of 30 healthy individuals matched with the patients in terms of age, gender, and race were used as a control group. The c.+3473A>G (rs3742330) polymorphism in the DICER1 gene and the c.-871C>T (rs9514828) single nucleotide polymorphism in the BAFF gene were examined using the PCR-RFLP method. In addition, the presence of mutations in the 11th and 25th exons of the DICER1 gene was evaluated by SANGER sequencing. The results of the control and patient groups were analyzed for polymorphism and mutation presence using Chi-square and Fisher tests.

Results: The polymorphic regions of c.+3473A>G(rs3742330) in the DICER1 gene and c.-871C>T (rs 9514828) in the BAFF gene were examined in the patient and control groups, but no statistically significant relationship was found. When the exon 11 and exon 25 of the DICER1 gene were investigated, no statistically significant relationship was found between the patient and control groups(p>0.05).

Conclusion: The absence of a difference between the patient and control groups suggests that different genetic mechanisms may be involved in the formation of B-NHL. The small population in the study is one of the reasons why no significant difference was found between the results. Additional studies are needed in larger patient and control groups.

Supporting Institution

This study was supported by Istanbul University Scientific Research Projects Unit (BAP)

Project Number

43912

Thanks

This study was supported by Istanbul University Scientific Research Projects Unit (BAP)

References

  • Stratton MR. Journeys into the genome of cancer cells. EMBO Mol Med 2013;5(2):169-72. google scholar
  • Thandra KC, Barsouk A, Saginala K, Padala SA, Barsouk A, Rawla P. Epidemiology of non-hodgkin’s lymphoma. Med Sci 2021;9(1):5- 14. google scholar
  • Erratum. Global cancer statistics 2018: Globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 2020;70(4):313. google scholar
  • Yildirim M, Karakilinc H, Yildiz M, Kurtoglu E, Dilli UD, Goktas S, et al. Non-hodgkin lymphoma and pesticide exposure in Turkey. Asian Pac J Cancer Prev 2013;14(6):3461-3. google scholar
  • Armitage JO, Weisenburger DD. New approach to classifying nonhodgkin’s lymphomas: clinical features of the major histologic subtypes. Non-hodgkin’s lymphoma classification project. J Clin Oncol 1998;16(8):2780-95. google scholar
  • Armitage JO, Gascoyne RD, Lunning MA, Cavalli F. Non-hodgkin lymphoma. Lancet 2017;390(10091):298-310. google scholar
  • Foulkes WD, Priest JR, Duchaine TF. DICER1: mutations, microRNAs and mechanisms. Nat Rev Cancer 2014;14(10):662-72. google scholar
  • Li X, Tian X, Zhang B, Zhang Y, Chen J. Variation in dicer gene is associated with increased survival in T-cell lymphoma. PLoS One 2012;7(12):e51640. google scholar
  • Di Lisio L, Martinez N, Montes-Moreno S, Piris-Villaespesa M, Sanchez-Beato M, Piris MA. The role of miRNAs in the pathogenesis and diagnosis of B-cell lymphomas. Blood 2012;120(9):1782-90. google scholar
  • Li X, Tian X, Zhang B, Chen J. Polymorphisms in microRNArelated genes are associated with survival of patients with T-cell lymphoma. Oncologist 2014;19(3):243-9. google scholar
  • Murray MJ, Bailey S, Raby KL, Saini HK, de Kock L, Burke GA, et al. Serum levels of mature microRNAs in DICER1-mutated pleuropulmonary blastoma. Oncogenesis 2014;3(2):e87. google scholar
  • Koizumi M, Hiasa Y, Kumagi T, Yamanishi H, Azemoto N, Kobata T, et al. Increased B cell-activating factor promotes tumor invasion and metastasis in human pancreatic cancer. PLoS One 2013;8(8):e71367. google scholar
  • Naradikian MS, Perate AR, Cancro MP. BAFF receptors and ligands create independent homeostatic niches for B cell subsets. Curr Opin Immunol 2015;34:126-9. google scholar
  • Yang S, Li JY, Xu W. Role of BAFF/BAFF-R axis in B-cell non-hodgkin lymphoma. Crit Rev Oncol Hematol 2014;91(2):113-22. google scholar
  • Li YJ, Jiang WQ, Rao HL, Huang JJ, Xia Y, Huang HQ, et al. Expression of BAFF and BAFF-R in follicular lymphoma: correlation with clinicopathologic characteristics and survival outcomes. PLoS One 2012;7(12):e50936. google scholar
  • Novak AJ, Grote DM, Stenson M, Ziesmer SC, Witzig TE, Habermann TM, et al. Expression of BLyS and its receptors in B-cell non-hodgkin lymphoma: correlation with disease activity and patient outcome. Blood 2004;104(8):2247-53. google scholar
  • Zhai K, Tian X, Wu C, Lu N, Chang J, Huang L, et al. Cytokine BAFF gene variation is associated with survival of patients with T-cell lymphomas. Clin Cancer Res 2012;18(8):2250-6. google scholar
There are 17 citations in total.

Details

Primary Language English
Subjects Medical Genetics (Excl. Cancer Genetics)
Journal Section Research Articles
Authors

Nurcan Cirak 0000-0003-0964-4817

Demet Akdeniz 0000-0002-2271-8481

Hülya Yazıcı

Project Number 43912
Publication Date February 15, 2024
Submission Date August 17, 2023
Published in Issue Year 2024 Volume: 7 Issue: 1

Cite

MLA Cirak, Nurcan et al. “INVESTIGATION OF DICER1 AND BAFF GENE MUTATIONS IN B-CELL NON-HODGKIN LYMPHOMA”. Sağlık Bilimlerinde İleri Araştırmalar Dergisi, vol. 7, no. 1, 2024, pp. 8-16, doi:10.26650/JARHS2023-1344811.