EFFECT OF CHRONIC STRESS ON SERUM AND TISSUE LEVELS OF KLOTHO AND TNF-α

Year 2024, Volume: 7 Issue: 2, 117 - 123, 04.06.2024

Mehmet Deniz Yener , Tuncay Çolak , Esra Acar , Fatma Ceyla Eraldemir

https://doi.org/10.26650/JARHS2024-1375216

Abstract

Objective: Chronic stress is recognized as a factor that affects almost all organs and tissues and disrupts homeostasis. Stress also directly impacts the inflammatory process. In addition, klotho is a protein associated with lifespan. This study investigates the relationship that chronic stress has with klotho and tumor necrosis factor alpha (TNF-α), which is a key protein in the proinflammatory process.

Materials and Methods: The study involves 16 Wistar albino rats divided into control and stress groups. The study applies the protocol for the chronic unpredictable mild stress (CUMS) model and uses the enzymelinked immunosorbent assay (ELISA) method to measure the TNF-α and Klotho levels in the serum, heart, aorta, and liver tissues.

Results: The serum samples obtained from the stress group were found to have a significant decrease in klotho levels (p=0.013) and high TNF-α levels (p=0.042). However, no significant difference was observed in the TNF-α or klotho levels in the heart, aorta, and liver tissues between groups.

Conclusion: Under chronic stress, a significant increase is observed in TNFα, one of the main cytokines of inflammation, as well as a decrease in klotho hormone. This research endeavors to contribute to the existing body of knowledge concerning the interplay among stress, inflammation, and longevity.

Keywords

Chronic stress, inflammation, klotho, TNF-α

Project Number

0742018

KRONİK STRESİN KLOTHO VE TNF-α’NIN SERUM VE DOKU SEVİYELERİ ÜZERİNE ETKİSİ

Abstract

Amaç: Kronik stres hemen hemen tüm organları/dokuları etkileyen ve homeostazı bozan bir faktör olarak kabul edilmektedir. Stres, enflamatuar süreç üzerinde doğrudan bir etkiye sahiptir. Bununla birlikte, Klotho yaşam süresi ile ilişkili bir proteindir. Çalışmamızda kronik stres ile proinflamatuar süreçte anahtar bir protein olan Tümör nekroz faktör alfa (TNF-α) ve Klotho arasındaki ilişkiyi araştırdık.

Gereç ve Yöntemler: Çalışmamız kontrol ve stres gruplarına ayrılan 16 Wistar albino’dan oluşmuştur. Kronik öngörülemeyen hafif stres model protokolü uygulandı. Serum, kalp, aort ve karaciğer dokusundaki TNF-α ve Klotho düzeyleri ELISA yöntemi kullanılarak ölçüldü.

Bulgular: Stres grubundan alınan serum örneklerinin Klotho düzeylerinde anlamlı azalma (p=0,013), TNF-α düzeylerinde ise anlamlı yükseliş (p=0,042) olduğu belirlendi. Ancak; gruplar arasında kalp, aort ve karaciğer dokularındaki TNF-α ve Klotho düzeylerinde anlamlı bir fark gözlenmedi.

Sonuç: Kronik stres altında inflamasyonun ana sitokinlerinden biri olan TNF-α’ da belirgin bir artış, Klotho hormonunda ise azalma gözlendi. Araştırmamız; stres, iltihaplanma ve uzun ömür arasındaki etkileşime ilişkin mevcut bilgi birikimine katkıda bulunmaya çalışmaktadır

Keywords

Kronik stres, inflamasyon, Klotho, TNF-α

Project Number

0742018

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