NOT PON1 L55M BUT ACE I/D VARIANT MIGHT BE A RISK FACTOR FOR OSCC IN THE TURKISH POPULATION

Year 2025, Volume: 8 Issue: 1, 15 - 20, 04.03.2025

Ayşe Feyda Nursal , Özge Gümüşay , Serbülent Yiğit , Nilüfer Kuruca , Mehmet Kemal Tümer

https://doi.org/10.26650/JARHS2025-1579437

Abstract

Objective: Oral squamous cell carcinoma (OSCC) covers more than 90% of the malignant neoplasms in the mouth. It has been shown that angiotensin-converting enzyme (ACE) and paraoxonase (PON1) gene variants were associated with several cancers. Therefore, we investigated the possible association between ACE insertion/deletion (I/D)-PON1 L55M variants and OSCC development risk in a Turkish population.

Material and Methods: A total of 155 people (104 healthy controls and 51 OSCC patients) made up the study population. These variants were genotyped using polymerase chain reaction (PCR) and/or restriction fragment length polymorphism (RFLP) assays.

Results: ACE I/D allele frequencies were significantly different between patients and controls. The ACE D allele was higher in the patient group compared to the control group, while the I allele was more prevalent in controls than patients (p˂0.0000001). When the patients and controls were examined based on the II+ID vs. DD genotype and II: ID vs. DD, a statistically significant correlation was found (p = 0.0000001 and p = 0.008691, respectively). The genotype and allele distribution of PON1 L55M did not significantly differ between the groups.

Conclusion: In conclusion, our study showed that the ACE I/D variant D allele is a risk factor for the development of OSCC in Turkey. This study contributes to more studies to confirm that ACE I/D plays a role as a genetic risk factor for OSCC.

Keywords

Oral squamous cell carcinoma, angiotensin-converting enzyme, paraoxonase, variant

TÜRK POPÜLASYONUNDA ACE I/D VARYANTI OSCC İÇİN BİR RİSK FAKTÖRÜ OLABİLİR FAKAT PON1 L55M RİSK FAKTÖRÜ DEĞİLDİR

Abstract

Amaç: Oral skuamöz hücreli karsinom (OSCC), ağızdaki malign neoplazm ların %90’ından fazlasını kapsar. Anjiyotensin dönüştürücü enzim (ACE) ve paraoksonaz (PON1) gen varyantlarının birçok kanserle ilişkili olduğu gös terilmiştir. Bu nedenle, Türk popülasyonunda ACE I/D-PON1 L55M var yantları ile OSCC gelişim riski arasındaki olası ilişkiyi araştırmayı amaçladık.

Gereç ve Yöntemler: Çalışma popülasyonu toplam 155 bireyden oluşmak taydı (51 OSCC hastası ve 104 sağlıklı kontrol). ACE I/D - PON1 L55M var yantları, PCR ve RFLP analizleri kullanılarak genotiplendi.

Bulgular: ACE I/D alel frekansları hastalar ve kontroller arasında anlamlı şekilde farklılık göstermiştir. ACE D aleli, hasta grubunda kontrol grubuna kıyasla daha yüksek iken, I aleli kontrollerde hastalara kıyasla daha fazlaydı (p˂0,0000001). Hastalar ile kontroller, II+ID vs. DD genotipine (p=0,0000001) ve II: ID vs. DD’ye (p=0,008691) göre karşılaştırıldığında istatistiksel olarak anlamlı bir ilişki gözlendi. PON1 L55M genotipi veya alel dağılımı açısından hastalar ve kontroller arasında anlamlı bir fark buluna madı (p>0,05).

Sonuç: Sonuç olarak çalışmamız ACE I/D varyant D alelinin Türkiye’de OSCC gelişimi için risk faktörü olduğunu gösterdi. Bu çalışma, ACE I/D’nin OSCC için genetik bir risk faktörü olarak rol oynadığını doğrulamak amacıyla yapılacak daha fazla araştırmaya katkı sağlamaktadır.

Keywords

Anjiyotensin dönüştürücü enzim, oral skuamöz hücre li karsinom, paraoksonaz, varyant

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