Adenosine deaminase and Guanase deaminase activities in serum of patients with rheumatoid Arthritis

Abstract

We investigated purin and primidin salvage pathways in rheumatoid arthritis (RA). For the aim, Adenosine deaminase (AD) and Guanosine deaminase (GD) activities in serum of patients with di!erent manifestations of the disease (n=32) were investigated and compared with the control group of healthy individuals (n=52). RA cases were classified with respect to their clinics and treatment. The ADA activities were significantly higher (p<0.01) in patients with RA than in control although GD activity levels were found to be lower (p<0.01). When compared their AD and GD levels amongst the classified RA groups, there was no di!erence in AD levels amongst cases. In conclusion, increased serum AD activities in patients with RA may be dependent on and reflect the increase in phagocytic activity of macrophages and maturation of T-lymphocytes. The results indicated that plasma AD and GA activity can be used for the diagnosis of RA disease to support clinical findings and as an index for disease.

Keywords

• -

References

1. Antonioli L, Colucci R, La Motta C, Tuccori M, Awwad O, Da Settimo F, Blandizzi C, Fornai M. 2012. Adenosine deaminase in the modulation of immune system and its potential as a novel target for treatment of inflammatory disorders. Curr Drug Targets. 13(6):842-162.
2. Arnett FC, Edworthy SM, Bloch DA. 1988. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum 31:315-324.
3. Bukulmez G, Akan T, Ciliu G. 2000. Serum adenosine deaminase levels in patients with psoriasis: a prospective case-control study. Eur J dermatol 10: 274-276.
4. Erel O, Kocyigit A, Gurel MS, Bulut V, Seyrek A, Ozdemir Y. 1998. Adenosine deaminase activities in sera, lymphocytes and granulocytes in patients with Cutaneous Leishmaniasis. Mem Inst Oswaldo Cruz 93: 491-494.
5. Erkiliç K, Evereklioglu C, Cekmen M, Ozkiris A, Duygulu F, Dogan H. 2003. Adenosine deaminase enzyme activity is increased and negatively correlates with catalase, superoxide dismutase and glutathione peroxidase in patients with Behçet's disease: original contributions/clinical and laboratory investigations. Mediators Inflamm 12:107-16.
6. Fox IH, Kelley WN 1978. The role of adenosine deaminase and 2'-deoxyadenosine in mammalian cells. Ann Rev Biochem 47: 655-686.
7. Gakis G, Calia GM, Naitana AG, Ortu AN, Contu A. 1991. Serum and pleural adenosine deaminase activity. Chest 99:1555-1556.
8. Giusti G, Galanti B. 1984. Adenosine deaminase: colorimetric method, p. 315- 323. In HV Bergmeyer Methods of Enzymatic Analsis Academic Press, New York.

9. Jones DD, Roberts EL, Davies AG. 1983. The estimation of serum guanosine deaminase activity in liver diseases. J Clin Chem Biochem 21: 835-840.
10. Kamanlı A, Nazıroğlu M, Aydilek N, Hacıevliyagil C. 2004. Plasma lipid peroxidation and antioxidant levels in patients with rheumatoid arthritis. Cell Biochem Funct. 22:53-57.
11. Katchamart W, Johnson S, Lin HJ, Phumethum V, Salliot C, Bombardier C. 2010. Predictors for remission in rheumatoid arthritis patients: A systematic review. Arthritis Care Res (Hoboken). 62(8):1128-1143.
12. Klarenbeek NB, Allaart CF, Kerstens PJ, Huizinga TW, Dijkmans BA. 2009. The best story: on strategy trials in rheumatoid arthritis. Curr Opin Rheumatol. 21:291-298.
13. Kobayashi F, Ikeda T, Marumo F, Sato C. 1993. Adenosine deaminase isoenzymes in liver disease, Am J Gastroenterol 88:266-271.
14. Le NT, Xue M, Castelnoble LA, Jackson CJ. 2007. The dual personalities of matrix metalloproteinases in inflammation. Front Biosci. 12:1475-1487.
15. Nardiello S, Russo M, Pizzella T, Galanti B. 1983. Di"erent levels of lymphocyte adenosine deaminase in active and inactive forms of chronic liver disease. J Clin Lab Immunol 11:177-180.
16. Onal S, Nazıroğlu M, Colak M, Bulut V, Flores-Arce MF. 2011. E"ects of di"erent medical treatments on serum copper, selenium and zinc levels in patients with rheumatoid arthritis. Biol Trace Elem Res. 142(3):447-55.
17. Sullivan J, Osborne WR, Wedgewood RJ. 1977. Adenosine deaminase activity in lymphocytes. Br J Haematol 37: 157-158.
18. Ungerer JP, Burger HM, Bissbort SH, Vermaak WJ. 1996. Adenosine deaminase isoenzymes in typhoid fever. Eur J Clin Microbiol Infect Dis 15: 510-512.
19. Valls V, Ena J, Roca V, Perez-Oteyza C, Angeles Figueredo M, Enriquez-deSalamanca R. 1990. Significance of adenosine deaminase measurement in sera of patients with HIV-1 infection. AIDS 4: 365-366.
20. Van Linden A, Eltzschig HK. 2007. Role of pulmonary adenosine during hypoxia: extracellular generation, signaling and metabolism by surface adenosine deaminase/CD26. Expert Opin Biol Ther. 7(9):1437-47.
21. Yazar M, Sarban S, Kocyigit A, Isikan UE. 2005. Synovial fluid and plasma selenium, copper, zinc, and iron concentrations in patients with rheumatoid arthritis and osteoarthritis. Biol Trace Elem Res. 106:123-132.
22. Zakeri Z, Izadi S, Niazi A, Bari Z, Zendeboodi S, Shakiba M, Mashhadi M, Narouie B, Ghasemi-Rad M. 2012. Comparison of adenosine deaminase levels in serum and synovial fluid between patients with rheumatoid arthritis and osteoarthritis. Int J Clin Exp Med. 2012;5(2):195-200.
23. Zamani B, Jamali R, Jamali A. 2012. Serum adenosine deaminase may predict disease activity in rheumatoid arthritis. Rheumatol Int. 32(7):1967-1975.