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Year 2010, Volume: 2 Issue: 2, 92 - 97, 14.08.2013

Abstract

Ascorbic Acid Mediated Iron Toxicity in Caco-2 Cells:Effects of Different Iron Species

Year 2010, Volume: 2 Issue: 2, 92 - 97, 14.08.2013

Abstract

Aim of the presented study was to investigate whether scorbic acid enhances iron uptake and/or toxicity in Caco-2 cells. Cells were incubated with 1.5 mM of different iron species combined with increasing doses of ascorbic acid (1-3 mM). Thereafter viability, membrane damage, lipid peroxidation, and intracellular iron concentrations were measured. No effects on iron-induced damage to cells were found regarding viability.
Lipid peroxidation significantly increased when ascorbic acid was added to cells incubated with FeSO. In 4 contrast lactate dehydrogenase (LDH)-release decreased by addition of ascorbic acid in Caco-2 cells treated with FeCl : nitrilotriacetic acid (NTA) and FeCl : citric acid. Iron uptake was enhanced by ascorbic acid, when 3 3
FeCl : citric acid (1:2) or FeSO were used.

We suggested that, especially dependent on the iron species used, 3 4 a combination of ascorbic acid and iron favours lipid peroxidation in Caco-2 cells. But on the other hand we also found beneficial effects of this type of co-treatment, like an enhanced iron uptake and decreased plasma membrane damage.

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Details

Primary Language English
Journal Section Original Articles
Authors

Cem Ekmekcioglu

Bettina Zodl

Susanne Humpeler

Michaela Zeiner

Claudia Gundacker This is me

İlse Steffan This is me

Publication Date August 14, 2013
Published in Issue Year 2010 Volume: 2 Issue: 2

Cite

APA Ekmekcioglu, C., Zodl, B., Humpeler, S., Zeiner, M., et al. (2013). Ascorbic Acid Mediated Iron Toxicity in Caco-2 Cells:Effects of Different Iron Species. Cell Membranes and Free Radical Research, 2(2), 92-97.
AMA Ekmekcioglu C, Zodl B, Humpeler S, Zeiner M, Gundacker C, Steffan İ. Ascorbic Acid Mediated Iron Toxicity in Caco-2 Cells:Effects of Different Iron Species. Cell Membranes and Free Radical Research. August 2013;2(2):92-97.
Chicago Ekmekcioglu, Cem, Bettina Zodl, Susanne Humpeler, Michaela Zeiner, Claudia Gundacker, and İlse Steffan. “Ascorbic Acid Mediated Iron Toxicity in Caco-2 Cells:Effects of Different Iron Species”. Cell Membranes and Free Radical Research 2, no. 2 (August 2013): 92-97.
EndNote Ekmekcioglu C, Zodl B, Humpeler S, Zeiner M, Gundacker C, Steffan İ (August 1, 2013) Ascorbic Acid Mediated Iron Toxicity in Caco-2 Cells:Effects of Different Iron Species. Cell Membranes and Free Radical Research 2 2 92–97.
IEEE C. Ekmekcioglu, B. Zodl, S. Humpeler, M. Zeiner, C. Gundacker, and İ. Steffan, “Ascorbic Acid Mediated Iron Toxicity in Caco-2 Cells:Effects of Different Iron Species”, Cell Membranes and Free Radical Research, vol. 2, no. 2, pp. 92–97, 2013.
ISNAD Ekmekcioglu, Cem et al. “Ascorbic Acid Mediated Iron Toxicity in Caco-2 Cells:Effects of Different Iron Species”. Cell Membranes and Free Radical Research 2/2 (August 2013), 92-97.
JAMA Ekmekcioglu C, Zodl B, Humpeler S, Zeiner M, Gundacker C, Steffan İ. Ascorbic Acid Mediated Iron Toxicity in Caco-2 Cells:Effects of Different Iron Species. Cell Membranes and Free Radical Research. 2013;2:92–97.
MLA Ekmekcioglu, Cem et al. “Ascorbic Acid Mediated Iron Toxicity in Caco-2 Cells:Effects of Different Iron Species”. Cell Membranes and Free Radical Research, vol. 2, no. 2, 2013, pp. 92-97.
Vancouver Ekmekcioglu C, Zodl B, Humpeler S, Zeiner M, Gundacker C, Steffan İ. Ascorbic Acid Mediated Iron Toxicity in Caco-2 Cells:Effects of Different Iron Species. Cell Membranes and Free Radical Research. 2013;2(2):92-7.