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Year 2011, Volume 3, Issue 1, 133 - 138, 14.08.2013

Abstract

References

  • Aitken RJ, Clakson JS. 1988. The generation of reactive oxygen species by human spermatozoa. In free radical: Chemistry, Pathology, Medicine, C.Rice- Evans and T. I. Dormandy, (ed.). London Richelieu 187 - 210.
  • Alan L, Miller ND. 1996. Antioxidant flavonoid, structure, function and Clinical Usage. Alternative Med 1: 103-111.
  • Ayuveda JP, Verpoorte R. 2005. Some phytochemical aspects of medicinal plants research. J Ethnopharmacol 25: 43-59.
  • Bors W, Saran M. 1991. Radical scavenging activity by flavonoid antioxidants. Hort science 26: 66-68.
  • Burkill HM. 1985. The useful plants of West Tropical Africa. 2nd (ed.), Volume 1. Royal Botanic Garden, Kew, Great Britain.
  • De Pasquale A. 1984. Pharmacognosy: The oldest modern science. J Ethnopharmacol 11: 1-16.
  • Frankel EN, Huang SW, Aeschbach R, Prior E. 1996. Antioxidant activity of a Rosemary extract and its constituents, carnosic acid, carnosol and rosemarinic acid in Bulk oil and oil - in - water emulsion. J Agric and Food Chem. 44: 131 - 135.
  • Gow-chin Y, Pin-Der D. 1994. Scavenging Effect of methanolic Extracts of Peanut Hulls on Free-Radical and Active-oxygen Species. J Agric Food Chem 42: 629 - 632.
  • Gulcin IM, Oktay OK, Aslan A. 2002. Determination of antioxidant activity of Linchen Cetraria islandica (L). Ach. J. Ethnopharmacol. 79: 325-329.
  • Halliwell B. 1989. Current Status Review. Free Radicals, reactive oxygen species and human diseases-a critical evaluation with special reference to atheroscelerosis. British J Exp Pathol 70: 737 - 757.
  • Halliwell, 2006. Oxygen toxicity, oxygen radicals and transition metals and disease. J Neurochem 97:1634-1658.
  • Hamburger M, Hostettmann K. 1991. Bioactivity in Plants: The link between phytochemistry and medicine. Phytochem 30 (12): 3864-3874.
  • Hatano T, Kagawa H, Yasuhora T, Okuta T. 1988. Two new flavonoids and other constituents in licorice root: Their relative astringency and radical scavenging effects. Chemical and Pharmaceutical Bullettin 36, 2090-209.
  • Kawazu K. 1972. Active constituents of piscicidal plants. Yuukigoscikagaku 30: 615-628.
  • Koleva II, Van - Beck TA. 2002. Evstaliva A. Screening of plant for antioxidant Activity. A comparative study on three testing methods. Phytochem Anal. 13: 8-17.
  • Lugasi A, Honvahorich P (deceased), Dworschark A. 1999. Additional information to the in-vitro, Antioxidant Activity of Ginkgo Biloba L. Phytother Res 13: 160-162.
  • Mensor LL, Menezes FS, Leitão GG, Reis AS, Tereca C, Coube CS, Leirao SG. 2001. Screening of Brazilian Plant Extracts for Antioxidant activity by the use of DPPH Free Radical method. Phytother Res 15: 127 - 130.
  • Mutee AF, Salhimi SM, Yam MF, Lim CP, Abdullah GZ, Ameer OZ, Abdulkareem MF, Asmawi MZ. 2010. In vivo anti-inflammatory and in vitro antioxidant activities of Peperomia pellucida. Int J Pharmacol 6: 686-690.
  • Nakayama T, Osawa T, Mendoza EMT, Lauena AC, Kawakishi S. 1994. Comparative study of Antioxidative Assays of Plant Materials, In Postharvest Biochemistry of Plant Food Materials in the Tropics, Uritani Ed. Jon. Science Society Press, Tokyo 83 - 94.
  • Namiki M. 1990. Antioxidant / antimutagens in Foods. Crit Rev Food Sci Nut 29 (4): 273 - 300.
  • Oloyede GK, Farombi OE, 2010. Antioxidant Properties Crinum ornatum Bulb Extract. World J Chem (5)1: 32-36.
  • Oloyede GK, Oke MJ, Raji Y, Olugbade AT. 2010. Antioxidant and anticonvulsant Alkaloids in Crinum ornatum Bulb Extract. World J Chem (5)1: 26-31.
  • Potterat O. 1997. Antioxidants and free radical scavengers of natural origin. Curr Org Chem 1: 415-425.
  • Soares JR, Dinis TC, Cohn AP, Almeida LM. 1997. Antioxidant activity of some extracts of Thymus zygis. Free Rad Res 26: 469-478.
  • Simson BB, Ogorzaly MC. 1995. Economic Botany Plants in Our World. McGrawHill, Inc. USA, 742-760.
  • Sofowora A. 2008. Medicinal plants of Traditional Medicine in Africa. Third (ed.) Spectrum Books, Ibadan, Nigeria, 181 – 199.
  • Vulto AG, Smet PAGM. 1988. Meyler’s side effects of Drugs In: Dukes, M.M.G., 11th (ed.). Elsevier, Amsterdam, 999-1005.
  • Williamson E, Okpako DT, Evans FJ. 1996. Selection, preparation and pharmacological evaluation of plant material, Wiley, Chichester, 85-130.
  • Yue-Zhong S. 1998. Recent natural products based drug development: A pharmaceutical Industry perspective. J Nat Prod 61: 1053-1071.

In vitro antioxidant activity of extracts from the leaves of Hura crepitans (Euphorbiaceae) - a comparison of two assay methods

Year 2011, Volume 3, Issue 1, 133 - 138, 14.08.2013

Abstract

Hura crepitans (Sandbox tree) of the family of Euphorbiaceae has enjoyed many ethnomedicinal applications but little is known about its chemistry and pharmacology. This research reports the in vitro antioxidant activity of this plant using two different assay methods. Scavenging effect on 2, 2-diphenylpicrylhydrazyl (DPPH) radical at 517 nm and on hydroxyl radical generated by hydrogen peroxide at 285 nm in a UV-Visible spectrophotometric assay. Butylatedhydroxyanisole (BHA), vitamin C and α-tocopherol were used as reference standards. There is generally decrease in absorption of DPPH caused by the extracts. The percent inhibition of the crude extract increases with a decrease in concentration in the DPPH photometric assay. The percent inhibition of hexane, ethylacetate and butanol fractions was low except for the butanol fraction (50.7 % at 1.0 mg/ml) when compared with standards vitamin C (90.8 % at 1.0 mg/ml) and BHA (95.4 % at 1.0 mg/ml). In the hydrogen peroxide assay however, the hexane, ethylacetate and butanol fractions scavenged hydroxyl radical more effectively than the standards. The crude extract possessed maximum % inhibition in both the DPPH and hydrogen peroxide free radicals. Therefore, H. crepitans has very weak activity as a hydrogen donor but its activity as hydroxyl radical scavenger is high when compared to standards used. This study suggests that the crude and fractions obtained from H. crepitans possess antioxidant activities which can counteract or prevent oxidative damage in biological systems caused by the presence of hydroxyl radical.

References

  • Aitken RJ, Clakson JS. 1988. The generation of reactive oxygen species by human spermatozoa. In free radical: Chemistry, Pathology, Medicine, C.Rice- Evans and T. I. Dormandy, (ed.). London Richelieu 187 - 210.
  • Alan L, Miller ND. 1996. Antioxidant flavonoid, structure, function and Clinical Usage. Alternative Med 1: 103-111.
  • Ayuveda JP, Verpoorte R. 2005. Some phytochemical aspects of medicinal plants research. J Ethnopharmacol 25: 43-59.
  • Bors W, Saran M. 1991. Radical scavenging activity by flavonoid antioxidants. Hort science 26: 66-68.
  • Burkill HM. 1985. The useful plants of West Tropical Africa. 2nd (ed.), Volume 1. Royal Botanic Garden, Kew, Great Britain.
  • De Pasquale A. 1984. Pharmacognosy: The oldest modern science. J Ethnopharmacol 11: 1-16.
  • Frankel EN, Huang SW, Aeschbach R, Prior E. 1996. Antioxidant activity of a Rosemary extract and its constituents, carnosic acid, carnosol and rosemarinic acid in Bulk oil and oil - in - water emulsion. J Agric and Food Chem. 44: 131 - 135.
  • Gow-chin Y, Pin-Der D. 1994. Scavenging Effect of methanolic Extracts of Peanut Hulls on Free-Radical and Active-oxygen Species. J Agric Food Chem 42: 629 - 632.
  • Gulcin IM, Oktay OK, Aslan A. 2002. Determination of antioxidant activity of Linchen Cetraria islandica (L). Ach. J. Ethnopharmacol. 79: 325-329.
  • Halliwell B. 1989. Current Status Review. Free Radicals, reactive oxygen species and human diseases-a critical evaluation with special reference to atheroscelerosis. British J Exp Pathol 70: 737 - 757.
  • Halliwell, 2006. Oxygen toxicity, oxygen radicals and transition metals and disease. J Neurochem 97:1634-1658.
  • Hamburger M, Hostettmann K. 1991. Bioactivity in Plants: The link between phytochemistry and medicine. Phytochem 30 (12): 3864-3874.
  • Hatano T, Kagawa H, Yasuhora T, Okuta T. 1988. Two new flavonoids and other constituents in licorice root: Their relative astringency and radical scavenging effects. Chemical and Pharmaceutical Bullettin 36, 2090-209.
  • Kawazu K. 1972. Active constituents of piscicidal plants. Yuukigoscikagaku 30: 615-628.
  • Koleva II, Van - Beck TA. 2002. Evstaliva A. Screening of plant for antioxidant Activity. A comparative study on three testing methods. Phytochem Anal. 13: 8-17.
  • Lugasi A, Honvahorich P (deceased), Dworschark A. 1999. Additional information to the in-vitro, Antioxidant Activity of Ginkgo Biloba L. Phytother Res 13: 160-162.
  • Mensor LL, Menezes FS, Leitão GG, Reis AS, Tereca C, Coube CS, Leirao SG. 2001. Screening of Brazilian Plant Extracts for Antioxidant activity by the use of DPPH Free Radical method. Phytother Res 15: 127 - 130.
  • Mutee AF, Salhimi SM, Yam MF, Lim CP, Abdullah GZ, Ameer OZ, Abdulkareem MF, Asmawi MZ. 2010. In vivo anti-inflammatory and in vitro antioxidant activities of Peperomia pellucida. Int J Pharmacol 6: 686-690.
  • Nakayama T, Osawa T, Mendoza EMT, Lauena AC, Kawakishi S. 1994. Comparative study of Antioxidative Assays of Plant Materials, In Postharvest Biochemistry of Plant Food Materials in the Tropics, Uritani Ed. Jon. Science Society Press, Tokyo 83 - 94.
  • Namiki M. 1990. Antioxidant / antimutagens in Foods. Crit Rev Food Sci Nut 29 (4): 273 - 300.
  • Oloyede GK, Farombi OE, 2010. Antioxidant Properties Crinum ornatum Bulb Extract. World J Chem (5)1: 32-36.
  • Oloyede GK, Oke MJ, Raji Y, Olugbade AT. 2010. Antioxidant and anticonvulsant Alkaloids in Crinum ornatum Bulb Extract. World J Chem (5)1: 26-31.
  • Potterat O. 1997. Antioxidants and free radical scavengers of natural origin. Curr Org Chem 1: 415-425.
  • Soares JR, Dinis TC, Cohn AP, Almeida LM. 1997. Antioxidant activity of some extracts of Thymus zygis. Free Rad Res 26: 469-478.
  • Simson BB, Ogorzaly MC. 1995. Economic Botany Plants in Our World. McGrawHill, Inc. USA, 742-760.
  • Sofowora A. 2008. Medicinal plants of Traditional Medicine in Africa. Third (ed.) Spectrum Books, Ibadan, Nigeria, 181 – 199.
  • Vulto AG, Smet PAGM. 1988. Meyler’s side effects of Drugs In: Dukes, M.M.G., 11th (ed.). Elsevier, Amsterdam, 999-1005.
  • Williamson E, Okpako DT, Evans FJ. 1996. Selection, preparation and pharmacological evaluation of plant material, Wiley, Chichester, 85-130.
  • Yue-Zhong S. 1998. Recent natural products based drug development: A pharmaceutical Industry perspective. J Nat Prod 61: 1053-1071.

Details

Primary Language English
Journal Section Original Articles
Authors

Ganiyat OLOYEDE

Publication Date August 14, 2013
Published in Issue Year 2011, Volume 3, Issue 1

Cite

Bibtex @ { sducmfrr221715, journal = {Cell Membranes and Free Radical Research}, issn = {1308-416X}, address = {}, publisher = {Hücresel Sinir Bilimleri ve Oksidatif Stres Derneği}, year = {2013}, volume = {3}, pages = {133 - 138}, doi = {}, title = {In vitro antioxidant activity of extracts from the leaves of Hura crepitans (Euphorbiaceae) - a comparison of two assay methods}, key = {cite}, author = {Oloyede, Ganiyat} }
APA Oloyede, G. (2013). In vitro antioxidant activity of extracts from the leaves of Hura crepitans (Euphorbiaceae) - a comparison of two assay methods . Cell Membranes and Free Radical Research , 3 (1) , 133-138 . Retrieved from https://dergipark.org.tr/en/pub/sducmfrr/issue/20745/221715
MLA Oloyede, G. "In vitro antioxidant activity of extracts from the leaves of Hura crepitans (Euphorbiaceae) - a comparison of two assay methods" . Cell Membranes and Free Radical Research 3 (2013 ): 133-138 <https://dergipark.org.tr/en/pub/sducmfrr/issue/20745/221715>
Chicago Oloyede, G. "In vitro antioxidant activity of extracts from the leaves of Hura crepitans (Euphorbiaceae) - a comparison of two assay methods". Cell Membranes and Free Radical Research 3 (2013 ): 133-138
RIS TY - JOUR T1 - In vitro antioxidant activity of extracts from the leaves of Hura crepitans (Euphorbiaceae) - a comparison of two assay methods AU - Ganiyat Oloyede Y1 - 2013 PY - 2013 N1 - DO - T2 - Cell Membranes and Free Radical Research JF - Journal JO - JOR SP - 133 EP - 138 VL - 3 IS - 1 SN - 1308-416X- M3 - UR - Y2 - 2021 ER -
EndNote %0 Cell Membranes and Free Radical Research In vitro antioxidant activity of extracts from the leaves of Hura crepitans (Euphorbiaceae) - a comparison of two assay methods %A Ganiyat Oloyede %T In vitro antioxidant activity of extracts from the leaves of Hura crepitans (Euphorbiaceae) - a comparison of two assay methods %D 2013 %J Cell Membranes and Free Radical Research %P 1308-416X- %V 3 %N 1 %R %U
ISNAD Oloyede, Ganiyat . "In vitro antioxidant activity of extracts from the leaves of Hura crepitans (Euphorbiaceae) - a comparison of two assay methods". Cell Membranes and Free Radical Research 3 / 1 (August 2013): 133-138 .
AMA Oloyede G. In vitro antioxidant activity of extracts from the leaves of Hura crepitans (Euphorbiaceae) - a comparison of two assay methods. Cell Membranes and Free Radical Research. 2013; 3(1): 133-138.
Vancouver Oloyede G. In vitro antioxidant activity of extracts from the leaves of Hura crepitans (Euphorbiaceae) - a comparison of two assay methods. Cell Membranes and Free Radical Research. 2013; 3(1): 133-138.
IEEE G. Oloyede , "In vitro antioxidant activity of extracts from the leaves of Hura crepitans (Euphorbiaceae) - a comparison of two assay methods", Cell Membranes and Free Radical Research, vol. 3, no. 1, pp. 133-138, Aug. 2013