Effect of parameters on removal of RB19 dye by laccase enzyme and kinetic evaluation

Deniz İzlen Çifçi; Rıza Atav; Yalçın Güneş; Elçin Güneş

Effect of parameters on removal of RB19 dye by laccase enzyme and kinetic evaluation

Abstract

Dye decolorization of RB19 using commercial laccase (Cerrena unicolor) enzyme was evaluated in this study. The effect of pH, laccase concentration, temperature, metal salt, carbon and nitrogen sources and solvents on the removal of RB19 dye were examined in detail. Highest RB19 removal with laccase (Cerrena unicolor) was obtained at pH 6, 40°C, 0.3 g/L enzyme concentration and 200 mg/L initial dye concentration. According to the Michaelis–Menten kinetics, the highest Vmax was 6.61 mg/L.min at these conditions. 0.25 mM of Cu2+ or Mn2+ enhanced the decolorization of RB19 but further increase in metal concentration decreased the removal of RB19. In the presence of carbon source such as glucose, starch and lactose, removal of RB19 by laccase was not significantly affected. However, in the presence of nitrogen sources such as urea and NH4Cl, the removal of RB19 by laccase was significantly reduced.

Keywords

References

[1] Epolito W.J., Yang H., Bottomley L.A., Pavlostathis S.G., (2008) Kinetics of zero-valent iron reductive transformation of the anthraquinone dye Reactive Blue 4, Journal of Hazardous Materials 160, 594-600.
[2] Lee Y.H., Pavlostathis S.G., (2004) Decolorization and toxicity of reactive anthraquinone textile dyes under methanogenic conditions, Water Research 38, 1838-1852
[3] Fanchiang J.-M., Tseng D.-H., (2009) Degradation of anthraquinone dye C.I. Reactive Blue 19 in aqueous solution by ozonation, Chemosphere 77, 214-221.
[4] Mechichi T., Mhiri N., Sayadi S., (2006) Remazol Brilliant Blue R decolourization by the laccase from Trametes trogii, Chemosphere 64, 998-1005.
[5] Soares G.M.B., Pessoa de Amorim M.T., Costa-Ferreira M., (2001) Use of laccase together with redox mediators to decolourize Remazol Brilliant Blue R, Journal of Biotechnology 89, 123-129.
[6] Rezaei S., Tahmasbi H., Mogharabi M., Ameri A., Forootanfar H., Khoshayand M.R., Faramarzi M.A., (2015) Laccase-catalyzed decolorization and detoxification of Acid Blue 92: statistical optimization, microtoxicity, kinetics, and energetics, Journal of Environmental Health Science & Engineering 13(31), 1-9.
[7] Cristóvão R.O., (2010) Degradation of dye-containing textile effluents by enzymatic catalysis, PhD Thesis, University of Porto, Portugal.
[8] Khlifi R., Belbahri L., Woodward S., Ellouz M., Dhouib A., Sayadi S., Mechichi T., (2010) Decolourization and detoxification of textile industry wastewater by the laccase-mediator system, Journal of Hazardous Materials 175, 802-808.

[9] Yeşilada Ö., Birhanlı E., Ercan S., Özmen N., (2014) Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii, Turkish Journal of Biology 38, 103-110.
[10] Wong Y., Yu J., (1999) Laccase-catalyzed decolorization of synthetic dyes, Water Research 33(16) 3512-3520.
[11] Çifçi D.İ., Atav R., Güneş Y., Güneş E., (2019) Determination of the color removal efficiency of laccase enzyme depending on dye class and chromophore, Water Science and Technology 80(1), 134-143.
[12] Murugesan K., Kim Y.-M., Jeon J.-R., Chang Y.-S., (2009) Effect of metal ions on reactive dye decolorization by laccase from Ganoderma lucidum, Journal of Hazardous Materials 168, 523-529.
[13] Sathishkumar P., Balan K., Palvannan T., Kamala-Kannan S., Oh B.-T., Rodríguez-Couto S., (2013) Efficiency of Pleurotus florida laccase on decolorization and detoxification of the reactive dye Remazol Brilliant Blue R (RBBR) under optimized conditions, Clean – Soil, Air, Water 41(7), 665-672.
[14] Russo M.E., Giardina P., Marzocchella A., Salatino P., Sannia G., (2008) Assessment of anthraquinone-dye conversion by free and immobilized crude laccase mixtures, Enzyme and Microbial Technology 42, 521-530.
[15] Palmieri G., Cennamo G., Sannia G., (2005) Remazol Brilliant Blue R decolourisation by the fungus Pleurotus ostreatus and its oxidative enzymatic system, Enzyme and Microbial Technology 36, 17-24.
[16] Erkurt E.A., Ünyayar A., Kumbur H., (2007) Decolorization of synthetic dyes by white rot fungi, involving laccase enzyme in the process, Process Biochemistry 42, 1429-1435.
[17] Moilanen U., Osma J.F., Winquist E., Leisola M., Rodríguez Couto S., (2010) Decolorization of simulated textile dye baths by crude laccases from Trametes hirsuta and Cerrena unicolor, Engineering in Life Science 10(3), 242-247.
[18] Yang J., Lin Q., Ng T.B., Ye X., Lin J., (2014) Purification and characterization of a novel laccase from Cerrena sp. HYB07 with dye decolorizing ability, PLoS ONE 9(10), e110834.
[19] Xu X., Huang X., Liu D., Lin J., Ye X., Yang J., (2018) Inhibition of metal ions on Cerrena sp. laccase: Kinetic, decolorization and fluorescence studies, Journal of the Taiwan Institute of Chemical Engineers 84, 1-10.
[20] Correia V.M., Stephenson T., Judd S.J., (1994) Characterisation of textile wastewaters ‐ a review, Environmental Technology 15(10), 917-929.
[21] Salazar-López M., de J. Rostro-Alanis M., Castillo-Zacarías C., Parra-Guardado A.L., Hernández-Luna C., Iqbal H.M.N., Parra-Saldivar R., (2017) Induced degradation of anthraquinone-based dye by laccase produced from Pycnoporus sanguineus (CS43), Water, Air, & Soil Pollution 228(469), 1-10.
[22] Patel A.M., Patel V.M., Pandya J., Trivedi U.B., Patel K.C., (2017) Evaluation of catalytic efficiency of Coriolopsis caperata DN laccase to decolorize and detoxify RBBR dye, Water Conservation Science and Engineering 2, 85-98.
[23] Tavares A.P.M., Cristóvão R.O, Loureiro J.M., Boaventura R.A.R., Macedo E.A., (2008) Optimisation of reactive textile dyes degradation by laccase–mediator system, Journal of Chemical Technology and Biotechnology 83, 1609-1615.
[24] Levin L., Melignani E., Ramos A.M., (2010) Effect of nitrogen sources and vitamins on ligninolytic enzyme production by some white-rot fungi. Dye decolorization by selected culture filtrates, Bioresource Technology 101, 4554-4563.
[25] Zeng X., Cai Y., Liao X., Zeng X., Li W., Zhang D., (2011) Decolorization of synthetic dyes by crude laccase from a newly isolated Trametes trogii strain cultivated on solid agro-industrial residue, Journal of Hazardous Materials 187, 517-525.
[26] Das A., Bhattacharya S., Panchanan G., Navya B.S., Nambiar P., (2016) Production, characterization and Congo red dye decolourizing efficiency of a laccase from Pleurotus ostreatus MTCC 142 cultivated on co-substrates of paddy straw and corn husk, Journal of Genetic Engineering and Biotechnology 14, 281-288.
[27] Deveci T., Unyayar A., Mazmanci M.A., (2004) Production of remazol brilliant blue R decolourising oxygenase from the culture filtrate of Funalia trogii ATCC 200800, Journal of Molecular Catalysis B: Enzymatic 30, 25-32.
[28] Hadibarata T., Yusoff A.R.M., Aris A., Salmiati, Hidayat T., Kristanti R.A., (2012) Decolorization of azo, triphenylmethane and anthraquinone dyes by laccase of a newly isolated Armillaria sp. F022, Water, Air, & Soil Pollution 223, 1045-1054.
[29] Wang S.-S., Ning Y.-J., Wang S.-N., Zhang J., Zhang G.-Q., Chen Q.-J. (2017), Purification, characterization, and cloning of an extracellular laccase with potent dye decolorizing ability from white rot fungus Cerrena unicolor GSM-01, International Journal of Biological Macromolecules 95, 920-927.
[30] Afreen S., Shamsi T.N., Baig M.A., Ahmad N., Fatima S., Qureshi M.I., Hassan M.I., Fatma T., (2017). A novel multicopper oxidase (laccase) from cyanobacteria: Purification, characterization with potential in the decolorization of anthraquinonic dye, PLoS ONE 12(4), e0175144.
[31] Nagai M., Sato T., Watanabe H., Saito K., Kawata M., Enei H., (2002). Purification and characterization of an extracellular laccase from the edible mushroom Lentinula edodes, and decolorization of chemically different dyes, Applied Microbiology and Biotechnology 60, 327-335.
[32] Shankar S., Nill S., (2015) Effect of metal ions and redox mediators on decolorization of synthetic dyes by crude laccase from a novel white rot fungus Peniophora sp. (NFCCI-2131), Applied Biochemistry and Biotechnology 175, 635-647.
[33] Dhakar K., Jain R., Tamta S., Pandey A., (2014) Prolonged laccase production by a cold and pH tolerant strain of Penicillium pinophilum (MCC 1049) isolated from a low temperature environment, Enzyme Research 120708, 1-6.
[34] Mikiashvili N., Wasser S.P., Nevo E., Elisashvil V., (2006) Effects of carbon and nitrogen sources on Pleurotus ostreatus ligninolytic enzyme activity, World Journal of Microbiology & Biotechnology 22, 999-1002.
[35] Michniewicz A., Ledakowicz S. , Ullrich R., Hofrichter M., (2008) Kinetics of the enzymatic decolorization of textile dyes by laccase from Cerrena unicolor, Dyes and Pigments 77, 295-302.
[36] Ashrafi S.D., Rezaei S., Forootanfar H., Mahvi A.H., Faramarzi M.A., (2013) The enzymatic decolorization and detoxification of synthetic dyes by the laccase from a soil-isolated ascomycete, Paraconiothyrium variabile, International Biodeterioration & Biodegradation 85, 173-181.
[37] Osma J.F., Toca-Herrera J.L., Rodriguez-Couto S., (2010) Transformation pathway of Remazol Brilliant Blue R by immobilised laccase, Bioresource Technology 101, 8509-8514.
[38] Zhuo R., Zhang J., Yu H., Ma F., Zhang X., (2019) The roles of Pleurotus ostreatus HAUCC 162 laccase isoenzymes in decolorization of synthetic dyes and the transformation pathways, Chemosphere 234, 733-745.
[39] Legerská B., Chmelovái D., Ondrejovič M., (2016) Degradation of synthetic dyes by laccases – a mini-review, Nova Biotechnologica et Chimica 15(1), 90-106.

Details

Primary Language

English

Subjects

Engineering

Journal Section

Research Article

Authors

Deniz İzlen Çifçi This is me
0000-0001-7527-6130
Türkiye

Rıza Atav This is me
0000-0002-5807-4542
Türkiye

Yalçın Güneş This is me
0000-0001-8697-3345
Türkiye

Elçin Güneş This is me
0000-0002-1457-1504
Türkiye

Publication Date

March 27, 2020

Submission Date

May 13, 2019

Acceptance Date

December 31, 2019

Published in Issue

Year 2020 Volume: 38 Number: 1

IZ

https://izlik.org/JA59NC46MC

Cite

RIS / Bibtex

APA

Çifçi, D. İ., Atav, R., Güneş, Y., & Güneş, E. (2020). Effect of parameters on removal of RB19 dye by laccase enzyme and kinetic evaluation. Sigma Journal of Engineering and Natural Sciences, 38(1), 371-382. https://izlik.org/JA59NC46MC

AMA

1.Çifçi Dİ, Atav R, Güneş Y, Güneş E. Effect of parameters on removal of RB19 dye by laccase enzyme and kinetic evaluation. SIGMA. 2020;38(1):371-382. https://izlik.org/JA59NC46MC

Chicago

Çifçi, Deniz İzlen, Rıza Atav, Yalçın Güneş, and Elçin Güneş. 2020. “Effect of Parameters on Removal of RB19 Dye by Laccase Enzyme and Kinetic Evaluation”. Sigma Journal of Engineering and Natural Sciences 38 (1): 371-82. https://izlik.org/JA59NC46MC.

EndNote

Çifçi Dİ, Atav R, Güneş Y, Güneş E (March 1, 2020) Effect of parameters on removal of RB19 dye by laccase enzyme and kinetic evaluation. Sigma Journal of Engineering and Natural Sciences 38 1 371–382.

IEEE

[1]D. İ. Çifçi, R. Atav, Y. Güneş, and E. Güneş, “Effect of parameters on removal of RB19 dye by laccase enzyme and kinetic evaluation”, SIGMA, vol. 38, no. 1, pp. 371–382, Mar. 2020, [Online]. Available: https://izlik.org/JA59NC46MC

ISNAD

Çifçi, Deniz İzlen - Atav, Rıza - Güneş, Yalçın - Güneş, Elçin. “Effect of Parameters on Removal of RB19 Dye by Laccase Enzyme and Kinetic Evaluation”. Sigma Journal of Engineering and Natural Sciences 38/1 (March 1, 2020): 371-382. https://izlik.org/JA59NC46MC.

JAMA

1.Çifçi Dİ, Atav R, Güneş Y, Güneş E. Effect of parameters on removal of RB19 dye by laccase enzyme and kinetic evaluation. SIGMA. 2020;38:371–382.

MLA

Çifçi, Deniz İzlen, et al. “Effect of Parameters on Removal of RB19 Dye by Laccase Enzyme and Kinetic Evaluation”. Sigma Journal of Engineering and Natural Sciences, vol. 38, no. 1, Mar. 2020, pp. 371-82, https://izlik.org/JA59NC46MC.

Vancouver

1.Deniz İzlen Çifçi, Rıza Atav, Yalçın Güneş, Elçin Güneş. Effect of parameters on removal of RB19 dye by laccase enzyme and kinetic evaluation. SIGMA [Internet]. 2020 Mar. 1;38(1):371-82. Available from: https://izlik.org/JA59NC46MC