Research Article

SOME MOLECULAR TECHNIQUES APPLIED in DETERMINATION of ENVIRONMENTAL MICROBIAL DIVERSITY

Volume: 33 Number: 1 March 1, 2015
  • Nihal Doğruöz Güngör

SOME MOLECULAR TECHNIQUES APPLIED in DETERMINATION of ENVIRONMENTAL MICROBIAL DIVERSITY

Abstract

The most important questions to be answered in the studies regarding the diversity of microorganisms in natural ecosystems are the functions of bacterial communities and how the compositions of these communities are affected by environmental changes. In order to answer these questions, there needs to be conducted advanced studies concerning the community structure. The total bacteria community studies require a huge amount of genetic data and high range of genetic diversity. Molecular techniques are quite valuable in researching the structure and diversity of bacterial communities. To combine various complementary molecular techniques is a nice strategy to keep track of microbial community changes in natural ecosystems. Combining some commonly-used techniques, i.e. polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), molecular cloning and fluorescence in situ hybridization (FISH), this review evaluates the advantages of using these techniques together in determining microbial diversity in environmental samples.

Keywords

References

  1. [1] V.L. Torsvik, Øvreås L. DNA Reassociation Yields Broad-Scale Information on Metagenome Complexity and Microbial Diversity. In: Handbook of Molecular Microbial Ecology I. John Wiley & Sons, Inc., 3-16, 2011.
  2. [2] J.M. Gonzalez, A. Ortiz-Martinez, M.A. Gonzalez-del Valle, L. Laiz, C. Saiz- Jimenez. An efficient strategy for screening large cloned libraries of amplified 16S rRNA sequences from complex environmental communities. J. Microbiol. Methods. 55: 459–46, 2003.
  3. [3] C. Schabereiter-Gurtner, G. Pinar, W. Lubitz, S. Rölleke. An advanced strategy to identify bacterial communities on art objects. J. Microbiol. Methods. 45: 77–87, 2001.
  4. [4] S.J. Giovannoni, T.B. Britschgi, C.L. Moyer, K.G. Field. Genetic diversity in Saragasso Sea Bacterioplankton. Nature. 345: 60-62, 1990.
  5. [5] D.L. Kirchman. Introduction and overview. In: Microbial Ecology of the Oceans. Wiley-Blackwell: New Jersey, 1-26, 2008.
  6. [6] R. Daniel. Soil-Based Metagenomics. In: Handbook of Molecular Microbial Ecology II: Metagenomics in Diff erent Habitats. Wiley-Blackwell, 83-92, 2011.
  7. [7] J.M. Gonzalez, C. Saiz-Jimenez. Unknown microbial communities on rock art painting. Consequences for conservation and future perspectives. Coalitio., 10: 4–7, 2005.
  8. [8] J. Wang, S. Krause, G. Muyzer, M. Meima-Franke, H. J. Laanbroek, P. L. Bodelier. Spatial patterns of iron-and methane-oxidizing bacterial communities in an irregularly flooded, riparian wetland. Frontiers in microbiology. 3: 1-13, 2012.

Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Nihal Doğruöz Güngör This is me
Türkiye

Publication Date

March 1, 2015

Submission Date

November 12, 2014

Acceptance Date

December 23, 2014

Published in Issue

Year 2015 Volume: 33 Number: 1

APA
Doğruöz Güngör, N. (2015). SOME MOLECULAR TECHNIQUES APPLIED in DETERMINATION of ENVIRONMENTAL MICROBIAL DIVERSITY. Sigma Journal of Engineering and Natural Sciences, 33(1), 54-64. https://izlik.org/JA22RD28KB
AMA
1.Doğruöz Güngör N. SOME MOLECULAR TECHNIQUES APPLIED in DETERMINATION of ENVIRONMENTAL MICROBIAL DIVERSITY. SIGMA. 2015;33(1):54-64. https://izlik.org/JA22RD28KB
Chicago
Doğruöz Güngör, Nihal. 2015. “SOME MOLECULAR TECHNIQUES APPLIED in DETERMINATION of ENVIRONMENTAL MICROBIAL DIVERSITY”. Sigma Journal of Engineering and Natural Sciences 33 (1): 54-64. https://izlik.org/JA22RD28KB.
EndNote
Doğruöz Güngör N (March 1, 2015) SOME MOLECULAR TECHNIQUES APPLIED in DETERMINATION of ENVIRONMENTAL MICROBIAL DIVERSITY. Sigma Journal of Engineering and Natural Sciences 33 1 54–64.
IEEE
[1]N. Doğruöz Güngör, “SOME MOLECULAR TECHNIQUES APPLIED in DETERMINATION of ENVIRONMENTAL MICROBIAL DIVERSITY”, SIGMA, vol. 33, no. 1, pp. 54–64, Mar. 2015, [Online]. Available: https://izlik.org/JA22RD28KB
ISNAD
Doğruöz Güngör, Nihal. “SOME MOLECULAR TECHNIQUES APPLIED in DETERMINATION of ENVIRONMENTAL MICROBIAL DIVERSITY”. Sigma Journal of Engineering and Natural Sciences 33/1 (March 1, 2015): 54-64. https://izlik.org/JA22RD28KB.
JAMA
1.Doğruöz Güngör N. SOME MOLECULAR TECHNIQUES APPLIED in DETERMINATION of ENVIRONMENTAL MICROBIAL DIVERSITY. SIGMA. 2015;33:54–64.
MLA
Doğruöz Güngör, Nihal. “SOME MOLECULAR TECHNIQUES APPLIED in DETERMINATION of ENVIRONMENTAL MICROBIAL DIVERSITY”. Sigma Journal of Engineering and Natural Sciences, vol. 33, no. 1, Mar. 2015, pp. 54-64, https://izlik.org/JA22RD28KB.
Vancouver
1.Nihal Doğruöz Güngör. SOME MOLECULAR TECHNIQUES APPLIED in DETERMINATION of ENVIRONMENTAL MICROBIAL DIVERSITY. SIGMA [Internet]. 2015 Mar. 1;33(1):54-6. Available from: https://izlik.org/JA22RD28KB

IMPORTANT NOTE: JOURNAL SUBMISSION LINK https://eds.yildiz.edu.tr/sigma/