STUDIES ON PRODUCTION OF LACCASE ENZYME BY WHITE ROT FUNGI

Abstract

In this study was investigated laccase activity from six white rot fungi collected from Yildiz Technical University Davutpasa Campus. Laccase enzyme is known to have produced as secondary metabolites by white rot fungi. The samples were taken from four different regions of fungi, the inner, outer, top hats and hats, including. The best storage conditions for fungi were detected. Fungi were cultivated in two different solid agar agar medium and are detected in which are fungi growth conditions the most active and without contamination. Then, pure cultures of fungi were inoculated into the two different containing trace elements and trace elements free laccase-production medium. Comparison by two different media the highest laccase activity were determined. In trace element-free medium with 5g/L-1 glucose, laccase activity was found to be 0.06 UmL-1. However, the highest laccase activity in medium containing trace elements and 10 gL-1 glucose were determined as 0.25 UmL-1.

Keywords

• White rot fungi
• laccase activity.
• LACCASE ENZYME

References

1. [1] Trametes Versicolor Beyaz Çürükçül Fungusundan Lakkaz Enziminin Saflaştırılması ve Kısmi Nitelendirilmesi, Yüksek Lisans Tezi, Fen Bilimleri Enstitüsü, GYTE, 2009.
2. [2] Brijwani K., Rigdon A., Vadlani P. V., Fungal Laccases: Production, Function, and Applications in Food Processing, Enzyme Research, (2010), 1-10, 2010.
3. [3] Desai S.S, Nityanand C., Microbial Laccases and Their Application: A Review, Asian Journal of Biochemistry, 3 (2), 98-124, 2011.
4. [4] Leonowicz A., Cho N.S., Luterek J., Wilkolazka A., Wasilewska M.W., Matuszewska A., Hofrichter M., Wesenberg D., Rogalski J., Fungal Laccases: Properties and activity on lignin Journal of Basic Microbiology, 41,( 3–4), 185–227,2001.
5. [5] Kunamneni A., Plou F. j., Ballesteros A., Alcalde M., Laccases and Their Applications, A Patent review, Recent Patents on Biotechnology, 2(1), 10-24, 2008.
6. [6] Pazarlıoğlu N.K., Sarıısık M., And Telefoncu A., Versicolor And Application To Denim Washing, Process Biochemistry, 40, 1673-1678,2005.
7. [7] Tien M., Kirk T., Lignin Degrading Enzyme from Phanerochaete crisosporium: Purification Characterization Catalicitc Properties of A Unique H2O2 Requiring Oxygenase, Proceedings of the National Academy of Science of the USA, 81, 2280-2284,1984.
8. [8] Bourbannis, R., Paice, M.G., Reid, I.D., Lanthier, P., Yaguchi, M., “Lignin Oxidation by Laccase Isozymes from Trametes versicolor and Role of the Mediatör ABTS in Kraft Lignin Depolymerization”, Applied and Environmental Microbiology 61,1876-1880, 1995.

9. [9] Bourbannis, R., Paice, M.G., Freiermuth, B., Bodie, E., Borneman, S., “Reactivities of Various Mediators and Laccases with Kraft Pulp and Lignin Model Compounds”, Applied and Environmental Microbiology, 63, 4627-4632,1997a.
10. [10] Wong Y., Yu,J., Laccase-Catalyzed Decolorization of Synthetic Dyes, Water Resource, 33: (16) 3512-3520, 1990.
11. [11] The Mycological Society of American Publishs Mycologia, Stanford University Libraries.
12. [12] Arda, M., Temel Mikrobiyoloji,Genişletilmiş ikinci baskı Medisan Yayın, Serisi No:46, 2000.
13. [13] Arısan Ataç I. , Peksel A., Biyoteknolojinin Temel İlkeleri ,YTÜ Basım-Yayın, Merkezi, İstanbul, 2006.
14. [14] Çotuk A., Genel Mikrobiyoloji Laboratuvar Yöntemleri, Nobel Tıp Kitabevi, İstanbul, 2003.
15. [15] Bilgehan H., Temel Mikrobiyoloji ve Bağışıklık Bilimi, 11. Baskı, Barış Yayınları, Fakülteler Kitabevi, 2005.