Investigation of using cave bacteria in the production of self-healing mortars

Elif Burcu Deliktaş; Mucteba Uysal; Nihal Doğruöz Güngör

Investigation of using cave bacteria in the production of self-healing mortars

Abstract

Self-healing concrete is a type of concrete formed by delayed or secondary hydration of cement and its particles with water in the matrix leaking through cracks, and calcium hydroxide and carbon dioxide on the crack surface react with calcium carbonate to provide protection against leakage through cracks. In recent studies, it has been observed that bacteria can self-repair with the production of calcium carbonate-based minerals and this repair provides protection against leakage, pore permeability and protection with increased strength and durability by reducing the infiltration of harmful substances into the internal structure. In this study, 32 different batches of samples were produced by using Bacillus thuringiensis, Pseudomonas putida and Sphingomonas mucosissima bacteria in mortar mixtures prepared by traditional methods with 2 different water/cement ratios and 3 different mineral additives. The bacteria used are cave isolates that provide calcium carbonate formation, and these isolates were multiplied in the appropriate growth culture and then added to the prepared mortar samples together with the mixture water. The specimens were cured with tap water for 28 days and then subjected to suction, ultrasonic, flexural and compressive strength tests. The results of the tests revealed a reduction in the porous structure of the specimens containing bacteria, resulting in lower water absorption rates compared to the reference specimens. It was observed that most CaCO3 was produced by Pseudomonas putida bacteria. However, there was no significant difference in compressive and flexural strengths compared to the reference specimens.

Keywords

References

REFERENCES
[1] Plan, Program, Raporlar ve Yayınlar | T.C. Sanayi ve Teknoloji Bakanlığı. 2015 https://www.sanayi.gov.tr/plan-program-raporlar-ve-yayinlar/sektor-raporlari/mu1203011413 Accesed on Nov 04, 2024.
[2] Karol RH. Chemical Grouting and Soil Stabilization. 3rd ed. New York: CRC Press; 2003. [CrossRef]
[3] Shaheen SM, Hooda PS, Tsadilas CD. Opportunities and challenges in the use of coal fly ash for soil improvements - A review. J Environ Manage 2014;145:249267. [CrossRef]
[4] Shackelford CD, Jefferis SA. Geoenvironmental engineering for in situ remediation. ISRM International Symposium 2000, IS 2000, Melbourne, Australia: 2018.
[5] Vekariya MS, Pitroda J. Bacterial concrete: New era for construction ındustry. Int J Eng Trends Technol 2013;4:4128–4137.
[6] Van Oss HG. Background Facts and Issues Concerning Cement and Cement Data Open-File Report 2005-1152, 2005. [CrossRef]
[7] Schlangen E, Joseph C. Self-Healing Processes in Concrete. In Ghosh SK, (Ed.). Self-Healing Materials. New Jersey, USA: Wiley‐VCH Verlag GmbH & Co. KGaA; 2008. [CrossRef]

[8] Yang Y, Lepech MD, Yang EH, Li VC. Autogenous healing of engineered cementitious composites under wet-dry cycles. Cem Concr Res 2009;39:382–390. [CrossRef]
[9] Kim JS, Schlangen E. Self-healing in ECC stimulated by SAP under flexural cyclic load. Proceedings of 3rd International Conference on Self Healing Materials, Bath, UK 2011.
[10] Snoeck D, Van Tittelboom K, De Belie N, Steuperaert S, Dubruel P. The use of superabsorbent polymers as a crack sealing and crack healing mechanism in cementitious materials. Concrete Repair, Rehabilitation and Retrofitting III - Proceedings of the 3rd International Conference on Concrete Repair, Rehabilitation and Retrofitting, ICCRRR 2012, 2012.
[11] Wang JY, Soens H, Verstraete W, De Belie N. Self-healing concrete by use of microencapsulated bacterial spores. Cem Concr Res 2014;56:139152. [CrossRef]
[12] Sierra-Beltran MG, Jonkers HM, Mera-Ortiz W. Field Application of Self-healing Concrete with Natural Fibres as Linings for Irrigation Canals in Ecuador. 2015.
[13] Sharma P. A study on self healing mechanism of microcracks in concrete structures using bacillus bacteria. Int J Sci Res Sci Eng Technol 2016;2:0613.
[14] Jonkers HM. Bacteria-based self-healing concrete. Heron J 2011;56:112.
[15] Wang J, Van Tittelboom K, De Belie N, Verstraete W. Use of silica gel or polyurethane immobilized bacteria for self-healing concrete. Constr Build Mater 2012;26:532–540. [CrossRef]
[16] Pei R, Liu J, Wang S, Yang M. Use of bacterial cell walls to improve the mechanical performance of concrete. Cem Concr Compos 2013;39:122130. [CrossRef]
[17] Jonkers HM, Thijssen A, Muyzer G, Copuroglu O, Schlangen E. Application of bacteria as self-healing agent for the development of sustainable concrete. Ecol Eng 2010;36.230235. [CrossRef]
[18] Wang JY, De Belie N, Verstraete W. Diatomaceous earth as a protective vehicle for bacteria applied for self-healing concrete. J Ind Microbiol Biotechnol 2012;39:567–577. [CrossRef]
[19] Wang JY, Snoeck D, Van Vlierberghe S, Verstraete W, De Belie N. Application of hydrogel encapsulated carbonate precipitating bacteria for approaching a realistic self-healing in concrete. Constr Build Mater 2014;68:110119. [CrossRef]
[20] Jonkers HM. Self Healing Concrete: A Biological Approach BT - Self Healing Materials: An Alternative Approach to 20 Centuries of Materials Science. Self Healing Materials 2007;30:195–204. [CrossRef]
[21] De Muynck W, Debrouwer D, De Belie N, Verstraete W. Bacterial carbonate precipitation improves the durability of cementitious materials. Cem Concr Res 2008;38:10051014. [CrossRef]
[22] Ramachandran SK, Ramakrishnan V, Bang SS. Remediation of concrete using micro-organisms. ACI Mater J 2001;98. [CrossRef]
[23] Achal V, Mukherjee A, Reddy MS. Microbial concrete: A way to enhance durability of building structures. 2nd International Conference on Sustainable Construction Materials and Technologies, 2010, p. 730–734. [CrossRef]
[24] Ghosh P, Mandal S, Chattopadhyay BD, Pal S. Use of microorganism to improve the strength of cement mortar. Cem Concr Res 2005;35:19801983. [CrossRef]
[25] Schwantes-Cezario N, Ferreira Nogueira Camargo GS, Franco do Couto Á, Porto MF, Cremasco LV, Andrello AC, et al. Mortars with the addition of bacterial spores: Evaluation of porosity using different test methods. J Build Eng 2020;30:101235. [CrossRef]
[26] Bhaskar S, Anwar Hossain KM, Lachemi M, Wolfaardt G, Otini Kroukamp M. Effect of self-healing on strength and durability of zeolite-immobilized bacterial cementitious mortar composites. Cem Concr Compos 2017;82:2333. [CrossRef]
[27] Xu J, Wang X. Self-healing of concrete cracks by use of bacteria-containing low alkali cementitious material. Constr Build Mater 2018;167:114. [CrossRef]
[28] Vashisht R, Shukla A. Potential application of bacteria to improve the self-healing and strength of concrete. Journal of Building Pathology and Rehabilitation 2020;5:10. [CrossRef]
[29] Yilmazer Polat B, Uysal M, Korkmaz V. A research for bacterial self-healing in metakaolin based geopolymer mortars. Sigma J Eng Nat Sci 2020;38:1401–1414.
[30] Yılmazer Polat B, Uysal M. Bacterial crack healing in metakaolin based geopolymer mortars. J Build Eng 2021;39:1–16. [CrossRef]
[31] Liu C, Lv Z, Xiao J, Xu X, Nong X, Liu H. On the mechanism of Cl− diffusion transport in self-healing concrete based on recycled coarse aggregates as microbial carriers. Cem Concr Compos 2021;124:104232. [CrossRef]
[32] Rais MS, Khan RA. Experimental investigation on the strength and durability properties of bacterial self-healing recycled aggregate concrete with mineral admixtures. Constr Build Mater 2021;306:124901. [CrossRef]
[33] Akhtar MK, Kanwal M, Khushnood RA, Khan MBE. Assessment of mechanical attributes and microstructural densification of self-healing recycled coarse aggregate concrete using various bacterial immobilizers. J Build Eng 2023;69:106229. [CrossRef]
[34] Jonkers HM, Schlangen E. Development of a bacteria-based self healing concrete. Proceedings of the International FIB Symposium 2008 - Tailor Made Concrete Structures: New Solutions for our Society, 2008. [CrossRef]
[35] Stanaszek-Tomal E. Bacterial concrete as a sustainable building material? Sustainability (Switzerland) 2020;12. [CrossRef]
[36] Zanjad N, Pawar S, Nayak C. Use of fly ash cenosphere in the construction Industry: A review. Mater Today Proc 2022;62:2185–2190. [CrossRef]
[37] Afolabi LO, Ariff ZM, Hashim SFS, Alomayri T, Mahzan S, Kamarudin KA, et al. Syntactic foams formulations, production techniques, and industry applications: a review. J Mater Res Technol 2020;9:10698–10718. [CrossRef]
[38] Patil L, Nayak CB, Jagadale UmeshT, Patil L, Nayak CB, Jagadale Umesh T. Effect of copper slag and fly ash and Nano Material to strengthen the properties of concrete. JETIR 2022;9:a831– a838.
[39] Yüksel I, Özkan Ö, Bilir T. Use of granulated blast-furnace slag in concrete as fine aggregate. ACI Mater J 2006;103:203208. [CrossRef]
[40] Aiswarya S, Prince Arulraj G, Dilip C. A review on use of metakaolin in concrete. Eng Sci Technol Int J 2013;3:1469714701.
[41] Başaran Bundur Z, Ferron R, Jo Kiristis M. Investigation of the Effect of Biomineralization on the Performance of Cement Based Materials, 9th National Concrete Congress, 2015. [Turkish]
[42] Harshali J Mitali S, Neha A, Pragati B. Bio concrete and bacteria based self healing concrete. Int J Res Eng Technol 2016;5:9599. [CrossRef]
[43] Hosseini Balam N, Mostofinejad D, Eftekhar M. Effects of bacterial remediation on compressive strength, water absorption, and chloride permeability of lightweight aggregate concrete. Constr Build Mater 2017;145:107116. [CrossRef]
[44] Hosseini Balam N, Mostofinejad D, Eftekhar M. Use of carbonate precipitating bacteria to reduce water absorption of aggregates. Constr Build Mater 2017;141:565577. [CrossRef]
[45] Jafarnia MS, Khodadad Saryazdi M, Moshtaghioun SM. Use of bacteria for repairing cracks and improving properties of concrete containing limestone powder and natural zeolite. Constr Build Mater 2020;242:118059. [CrossRef]
[46] Singh H, Gupta R. Cellulose fiber as bacteria-carrier in mortar: Self-healing quantification using UPV. J Build Eng 2020;28:e00324. [CrossRef]
[47] Kaur NP, Majhi S, Dhami NK, Mukherjee A. Healing fine cracks in concrete with bacterial cement for an advanced non-destructive monitoring. Constr Build Mater 2020;242:118151.
[48] Jonkers HM, Schlangen E. Self-healing of cracked concrete: A bacterial approach. Proceedings of the 6th International Conference on Fracture Mechanics of Concrete and Concrete Structures, vol. 3, Catania, Italy: 2007, p. 1821–1826. [CrossRef]
[49] Ghosh S, Biswas M, Chattopadhyay BD, Mandal S. Microbial activity on the microstructure of bacteria modified mortar. Cem Concr Compos 2009;31:4855.
[50] Hussain AZ, Tom A, Sasi CK, Joseph J, Joseph S. Microbial concrete and ınfluence of microbes on properties of concrete. Int J Sci Res 2017;3:744751.

Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Elif Burcu Deliktaş ^* This is me
0000-0002-5013-0693
Türkiye

Mucteba Uysal This is me
0000-0002-6827-9904
Türkiye

Nihal Doğruöz Güngör
0000-0002-8098-039X
Türkiye

Publication Date

December 9, 2024

Submission Date

July 9, 2023

Acceptance Date

October 11, 2023

Published in Issue

Year 2024 Volume: 42 Number: 6

IZ

https://izlik.org/JA55TG28PF

Cite

RIS / Bibtex

APA

Deliktaş, E. B., Uysal, M., & Doğruöz Güngör, N. (2024). Investigation of using cave bacteria in the production of self-healing mortars. Sigma Journal of Engineering and Natural Sciences, 42(6), 1712-1728. https://izlik.org/JA55TG28PF

AMA

1.Deliktaş EB, Uysal M, Doğruöz Güngör N. Investigation of using cave bacteria in the production of self-healing mortars. SIGMA. 2024;42(6):1712-1728. https://izlik.org/JA55TG28PF

Chicago

Deliktaş, Elif Burcu, Mucteba Uysal, and Nihal Doğruöz Güngör. 2024. “Investigation of Using Cave Bacteria in the Production of Self-Healing Mortars”. Sigma Journal of Engineering and Natural Sciences 42 (6): 1712-28. https://izlik.org/JA55TG28PF.

EndNote

Deliktaş EB, Uysal M, Doğruöz Güngör N (December 1, 2024) Investigation of using cave bacteria in the production of self-healing mortars. Sigma Journal of Engineering and Natural Sciences 42 6 1712–1728.

IEEE

[1]E. B. Deliktaş, M. Uysal, and N. Doğruöz Güngör, “Investigation of using cave bacteria in the production of self-healing mortars”, SIGMA, vol. 42, no. 6, pp. 1712–1728, Dec. 2024, [Online]. Available: https://izlik.org/JA55TG28PF

ISNAD

Deliktaş, Elif Burcu - Uysal, Mucteba - Doğruöz Güngör, Nihal. “Investigation of Using Cave Bacteria in the Production of Self-Healing Mortars”. Sigma Journal of Engineering and Natural Sciences 42/6 (December 1, 2024): 1712-1728. https://izlik.org/JA55TG28PF.

JAMA

1.Deliktaş EB, Uysal M, Doğruöz Güngör N. Investigation of using cave bacteria in the production of self-healing mortars. SIGMA. 2024;42:1712–1728.

MLA

Deliktaş, Elif Burcu, et al. “Investigation of Using Cave Bacteria in the Production of Self-Healing Mortars”. Sigma Journal of Engineering and Natural Sciences, vol. 42, no. 6, Dec. 2024, pp. 1712-28, https://izlik.org/JA55TG28PF.

Vancouver

1.Elif Burcu Deliktaş, Mucteba Uysal, Nihal Doğruöz Güngör. Investigation of using cave bacteria in the production of self-healing mortars. SIGMA [Internet]. 2024 Dec. 1;42(6):1712-28. Available from: https://izlik.org/JA55TG28PF