Isolation of kerosene-degrading bacteria from soil samples and determination of optimal growth conditions

Yerlan Bekeshev; Zhazira Zhumabekova; Laila Jumabayeva; Zhanna Suimenbayeva

Isolation of kerosene-degrading bacteria from soil samples and determination of optimal growth conditions

Abstract

Bacteria capable of decomposing hydrocarbon rocket power kerosene T-1 were isolated and studied. In the course of the studies, 30 isolates of microbial cultures were isolated from soils contaminated with hydrocarbon rocket fuel, of which 9 isolates were selected that actively as-similate kerosene T-1 as the only headwater of carbon. The strains that showed the best results in these screening analyses on four nutrient media with a concentration of T-1 kerosene 1% (10 g/kg) grow well 9 isolates of culture microorganisms: № 4, 8, 14, 23, 5, 18, 20, 25 and yeast № 12/5. Isolates on a medium with a concentration of T-1 kerosene 2% (20 g/kg) and 5% (50 g/kg) showed good growth in bacterial cultures № 5, 18, 20, 25, and yeast № 12/5. The selected microorganisms were identified by physiological and biochemical characteristics: № 5 – Arthrobacter sp., №18 – Acinetobacter calcoaceticum, №20 – Bacillus sp., №25- Micrococcus roseus, № 12/5- Candida sp. Cultivation conditions for isolated microorganisms were created. It has been established that the optimal development temperature for the culture of Arthrobacter sp. 5 is 25-30°C, Acinetobacter calcoaceticum. 18 is 30-35 °С , Micrococcus roseus. 25 is 25-37 °С. The opti-mal duration of cultivation of Candida sp. 12/5 is 1 day, for the rest of the studied cultures - 2 days.

Keywords

References

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Yerlan Bekeshev This is me
0000-0002-5248-5025
Kazakhstan

Zhazira Zhumabekova This is me
0000-0003-1854-798X
Kazakhstan

Laila Jumabayeva This is me
0000-0003-0105-2286
Kazakhstan

Zhanna Suimenbayeva ^* This is me
0000-0002-2347-356X
Kazakhstan

Publication Date

December 9, 2024

Submission Date

July 10, 2023

Acceptance Date

October 13, 2023

Published in Issue

Year 2024 Volume: 42 Number: 6

IZ

https://izlik.org/JA46KW44AJ

Cite

RIS / Bibtex

APA

Bekeshev, Y., Zhumabekova, Z., Jumabayeva, L., & Suimenbayeva, Z. (2024). Isolation of kerosene-degrading bacteria from soil samples and determination of optimal growth conditions. Sigma Journal of Engineering and Natural Sciences, 42(6), 1954-1964. https://izlik.org/JA46KW44AJ

AMA

1.Bekeshev Y, Zhumabekova Z, Jumabayeva L, Suimenbayeva Z. Isolation of kerosene-degrading bacteria from soil samples and determination of optimal growth conditions. SIGMA. 2024;42(6):1954-1964. https://izlik.org/JA46KW44AJ

Chicago

Bekeshev, Yerlan, Zhazira Zhumabekova, Laila Jumabayeva, and Zhanna Suimenbayeva. 2024. “Isolation of Kerosene-Degrading Bacteria from Soil Samples and Determination of Optimal Growth Conditions”. Sigma Journal of Engineering and Natural Sciences 42 (6): 1954-64. https://izlik.org/JA46KW44AJ.

EndNote

Bekeshev Y, Zhumabekova Z, Jumabayeva L, Suimenbayeva Z (December 1, 2024) Isolation of kerosene-degrading bacteria from soil samples and determination of optimal growth conditions. Sigma Journal of Engineering and Natural Sciences 42 6 1954–1964.

IEEE

[1]Y. Bekeshev, Z. Zhumabekova, L. Jumabayeva, and Z. Suimenbayeva, “Isolation of kerosene-degrading bacteria from soil samples and determination of optimal growth conditions”, SIGMA, vol. 42, no. 6, pp. 1954–1964, Dec. 2024, [Online]. Available: https://izlik.org/JA46KW44AJ

ISNAD

Bekeshev, Yerlan - Zhumabekova, Zhazira - Jumabayeva, Laila - Suimenbayeva, Zhanna. “Isolation of Kerosene-Degrading Bacteria from Soil Samples and Determination of Optimal Growth Conditions”. Sigma Journal of Engineering and Natural Sciences 42/6 (December 1, 2024): 1954-1964. https://izlik.org/JA46KW44AJ.

JAMA

1.Bekeshev Y, Zhumabekova Z, Jumabayeva L, Suimenbayeva Z. Isolation of kerosene-degrading bacteria from soil samples and determination of optimal growth conditions. SIGMA. 2024;42:1954–1964.

MLA

Bekeshev, Yerlan, et al. “Isolation of Kerosene-Degrading Bacteria from Soil Samples and Determination of Optimal Growth Conditions”. Sigma Journal of Engineering and Natural Sciences, vol. 42, no. 6, Dec. 2024, pp. 1954-6, https://izlik.org/JA46KW44AJ.

Vancouver

1.Yerlan Bekeshev, Zhazira Zhumabekova, Laila Jumabayeva, Zhanna Suimenbayeva. Isolation of kerosene-degrading bacteria from soil samples and determination of optimal growth conditions. SIGMA [Internet]. 2024 Dec. 1;42(6):1954-6. Available from: https://izlik.org/JA46KW44AJ