DIFFERENTIAL EXPRESSION OF BDNF, MIR-206 AND MIR-9 UNDER THE CHRONIC ETHANOL EXPOSURE AND ITS WITHDRAWAL

Year 2016, Volume: 34 Issue: 2, 191 - 196, 01.06.2016

Zeynep Aydın Sinirlioğlu Fahri Akbaş

Abstract

Chronic and acute alcohol abuse results in alters in the expression of some miRNAs and their mRNA targets in specific area of the brain. These expression changes might cause the cellular adjusments to long term alcohol abuse. We searched BDNF, miR-9 and miR-206 expression changed in SH SY5Y cell following exposure to ethanol and withdrawal. These miRNAs and BDNF are estimated to target genes of alcoholism. Chronic ethanol exposure and its removal showed that specific changes in miRNA and BDNF expression in cell line advocating that different expressions could be evoked with different exposure conditions. Especially, there were three group in our experiment one of them 10 days Chronic ethanol exposure, other one 5 days chronic ethanol exposure then 5 days normal medium without ethanol for withdrawal period and other group was control. After all, mir-9 was up-regulated after ethanol treatment but was downregulated in withdrawal condition. In addition mir-206 was up-regulated all condition. Whereas BDNF gene expression was regulated opposite direction according to miRNA expression. Our results suggest that a possible role of differentially expressed miRNAs and BDNF perform together in mediating alcohol influence.

Keywords

BDNF, miRNA, ethanol exposure, SHSY5.

References

• 1] Hofer M, Pagliusi SR, Hohn A, Leibrock J, Barde Y (1990) Regional distribution of brain-derived neurotrophic factor mRNA in the adult mouse brain. The EMBO journal 9 (8):2459.
• [2] Logrip ML, Janak PH, Ron D (2009) Escalating ethanol intake is associated with altered corticostriatal BDNF expression. Journal of neurochemistry 109 (5):1459-1468.
• [3] Lu B (2003) BDNF and activity-dependent synaptic modulation. Learning & memory 10 (2):86-98.
• [4] Bramham CR, Messaoudi E (2005) BDNF function in adult synaptic plasticity: the synaptic consolidation hypothesis. Progress in neurobiology 76 (2):99-125.
• [5] Tyler WJ, Alonso M, Bramham CR, Pozzo-Miller LD (2002) From acquisition to consolidation: on the role of brain-derived neurotrophic factor signaling in hippocampal-dependent learning. Learning & memory 9 (5):224-237.
• [6] Yamada K, Mizuno M, Nabeshima T (2002) Role for brain-derived neurotrophic factor in learning and memory. Life sciences 70 (7):735-744.
• [7] Yamada MK, Nakanishi K, Ohba S, Nakamura T, Ikegaya Y, Nishiyama N, Matsuki N (2002) Brain-derived neurotrophic factor promotes the maturation of GABAergic mechanisms in cultured hippocampal neurons. The Journal of Neuroscience 22 (17):7580-7585.
• [8] Russo SJ, Mazei-Robison MS, Ables JL, Nestler EJ (2009) Neurotrophic factors and structural plasticity in addiction. Neuropharmacology 56:73-82.
• [9] Pandey SC, Zhang H, Roy A, Misra K (2006) Central and medial amygdaloid brain-derived neurotrophic factor signaling plays a critical role in alcohol-drinking and anxiety-like behaviors. The Journal of Neuroscience 26 (32):8320-8331.
• [10] Prakash A, Zhang H, Pandey SC (2008) Innate Differences in the Expression of Brain‐Derived Neurotrophic Factor in the Regions Within the Extended Amygdala Between Alcohol Preferring and Nonpreferring Rats. Alcoholism: Clinical and Experimental Research 32 (6):909-920.
• [11] Tapocik JD, Solomon M, Flanigan M, Meinhardt M, Barbier E, Schank J, Schwandt M, Sommer WH, Heilig M (2013) Coordinated dysregulation of mRNAs and microRNAs in the rat medial prefrontal cortex following a history of alcohol dependence. The pharmacogenomics journal 13 (3):286-296.
• [12] Yan Q-S, Feng M-J, Yan S-E (2005) Different expression of brain-derived neurotrophic factor in the nucleus accumbens of alcohol-preferring (P) and-nonpreferring (NP) rats. Brain research 1035 (2):215-218.
• [13] Meinhardt MW, Hansson AC, Perreau-Lenz S, Bauder-Wenz C, Stählin O, Heilig M, Harper C, Drescher KU, Spanagel R, Sommer WH (2013) Rescue of infralimbic mGluR2 deficit restores control over drug-seeking behavior in alcohol dependence. The Journal of Neuroscience 33 (7):2794-2806.
• [14] Melendez RI, McGinty JF, Kalivas PW, Becker HC (2012) Brain region‐specific gene expression changes after chronic intermittent ethanol exposure and early withdrawal in C57BL/6J mice. Addiction biology 17 (2):351-364.
• [15] Tapocik JD, Barbier E, Flanigan M, Solomon M, Pincus A, Pilling A, Sun H, Schank JR, King C, Heilig M (2014) microRNA-206 in rat medial prefrontal cortex regulates BDNF expression and alcohol drinking. The Journal of Neuroscience 34 (13):4581-4588.
• [16] Lewohl JM, Nunez YO, Dodd PR, Tiwari GR, Harris RA, Mayfield RD (2011) Up‐Regulation of MicroRNAs in Brain of Human Alcoholics. Alcoholism: Clinical and Experimental Research 35 (11):1928-1937.
• [17] Lee ST, Chu K, Jung KH, Kim JH, Huh JY, Yoon H, Park DK, Lim JY, Kim JM, Jeon D (2012) miR‐206 regulates brain‐derived neurotrophic factor in Alzheimer disease model. Annals of neurology 72 (2):269-277.
• [18] Miura P, Amirouche A, Clow C, Bélanger G, Jasmin BJ (2012) Brain‐derived neurotrophic factor expression is repressed during myogenic differentiation by miR‐206. Journal of neurochemistry 120 (2):230-238.
• [19] Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB (1987) Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer research 47 (4):936-942.
• [20] Yücel S, Aydın Sinirlioğlu Z, Karakuzu B, Temel TM, Elalmış Y, Özarslan AC Production, Characterization and Bioactivity of 46S6 Bioactive Glass from Rice Hull Ash Silica. In: Advanced Materials Research, 2015. Trans Tech Publ, pp 857-861.
• [21] Thibault C, Lai C, Wilke N, Duong B, Olive MF, Rahman S, Dong H, Hodge CW, Lockhart DJ, Miles MF (2000) Expression profiling of neural cells reveals specific patterns of ethanol-responsive gene expression. Molecular Pharmacology 58 (6):1593-1600.
• [22] Williams AH, Valdez G, Moresi V, Qi X, McAnally J, Elliott JL, Bassel-Duby R, Sanes JR, Olson EN (2009) MicroRNA-206 delays ALS progression and promotes regeneration of neuromuscular synapses in mice. Science 326 (5959):1549-1554.
• [23] Sadri-Vakili G, Kumaresan V, Schmidt HD, Famous KR, Chawla P, Vassoler FM, Overland RP, Xia E, Bass CE, Terwilliger EF (2010) Cocaine-induced chromatin remodeling increases brain-derived neurotrophic factor transcription in the rat medial prefrontal cortex, which alters the reinforcing efficacy of cocaine. The Journal of Neuroscience 30 (35):11735-11744.
• [24] Nagappan G, Lu B (2005) Activity-dependent modulation of the BDNF receptor TrkB: mechanisms and implications. Trends in neurosciences 28 (9):464-471.
• [25] Pietrzykowski AZ, Friesen RM, Martin GE, Puig SI, Nowak CL, Wynne PM, Siegelmann HT, Treistman SN (2008) Posttranscriptional regulation of BK channel splice variant stability by miR-9 underlies neuroadaptation to alcohol. Neuron 59 (2):274-287.