Isolation of kerosene-degrading bacteria from soil samples and determination of optimal growth conditions

Year 2024, Volume: 42 Issue: 6, 1954 - 1964, 09.12.2024

Yerlan Bekeshev Zhazira Zhumabekova Laila Jumabayeva Zhanna Suimenbayeva

Abstract

Bacteria capable of decomposing hydrocarbon rocket power kerosene T-1 were isolated and studied. In the course of the studies, 30 isolates of microbial cultures were isolated from soils contaminated with hydrocarbon rocket fuel, of which 9 isolates were selected that actively as-similate kerosene T-1 as the only headwater of carbon. The strains that showed the best results in these screening analyses on four nutrient media with a concentration of T-1 kerosene 1% (10 g/kg) grow well 9 isolates of culture microorganisms: № 4, 8, 14, 23, 5, 18, 20, 25 and yeast № 12/5. Isolates on a medium with a concentration of T-1 kerosene 2% (20 g/kg) and 5% (50 g/kg) showed good growth in bacterial cultures № 5, 18, 20, 25, and yeast № 12/5.
The selected microorganisms were identified by physiological and biochemical characteristics: № 5 – Arthrobacter sp., №18 – Acinetobacter calcoaceticum, №20 – Bacillus sp., №25- Micrococcus roseus, № 12/5- Candida sp. Cultivation conditions for isolated microorganisms were created. It has been established that the optimal development temperature for the culture of Arthrobacter sp. 5 is 25-30°C, Acinetobacter calcoaceticum. 18 is 30-35 °С , Micrococcus roseus. 25 is 25-37 °С. The opti-mal duration of cultivation of Candida sp. 12/5 is 1 day, for the rest of the studied cultures - 2 days.

Keywords

Cultivation, Microorganism, Soil Pollution, Strain, T-1 Kerosene

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