HISTOLOGICAL EVALUATION OF THE RAT CRANIAL REGION

Abstract

Purpose: This study was to ascertain the histological evaluation of some organs in the cranial region of newborn Wistar albino rats, such as the tongue, palate, and Steno's gland in the nasal cavity, compared to adult rats. Methods: 5 healthy female newborn Wistar albino rats were used in the study. Tissues obtained from rats were fixed in 10% formalin for 2 days. The histological features of the tongue, palate and nasal cavity were revealed after Hematoxylin&eosin and Mallory’s Azan stainings. Results: While there was fatty tissue in the submucosa layer in the ventral section of the soft palate, mucus glands were observed in the dorsal section. The main papillae were filiform and fungiform, and scattered foliate and circumvallate papillae were also present. The lateral nasal glands (Steno’s glands) were well developed. In general, there were four ethmoturbinates in coronal and sagittal sections in the posterosuperior part of the unilateral sinus in adults, whereas there were two in neonatal rats. Unusually, a septal window was visible immediately rostral to the nasopharynx in the mouse, however, no septal window was observed in newborn rats. Conslusion: This study reports basic research features on the anatomy of the oral and nasal cavity of the newborn Wistar albino rat. Our data may shed light on other studies aimed at fully investigating the structure of these organs, which may be useful in subsequent experimental and morphological studies on newborn Wistar albino rats.

Keywords

• Cranial region
• Steno’s gland
• Rat

SIÇAN KRANİAL BÖLGESİNİN HİSTOLOJİK OLARAK DEĞERLENDİRİLMESİ

Öz

Amaç: Bu çalışma, neonatal Wistar albino sıçanların kranial bölgesindeki dil, damak ve burun boşluğundaki Steno bezi gibi bazı organların yetişkin sıçanlarla karşılaştırıldığında histolojik olarak değerlendirilmesini belirlemek amacıyla yapıldı. Yöntem: Çalışmada 5 adet sağlıklı dişi yenidoğan Wistar albino sıçan kullanıldı. Sıçanlardan elde edilen numuneler %10'luk formalinde iki gün süreyle fikse edildi. Hematoksilen&eozin ve Mallary Azan boyama sonrasında dil, damak ve burun boşluğunun histolojik özellikleri ortaya çıkarıldı. Bulgular: Yumuşak damağın ön kısmındaki submukoza tabakasında yağ dokusu bulunurken, arka kısmında mukus bezleri gözlendi. Ana papillalar filiform ve fungiform olup, dağınık foliat ve sirkumvallat papillalar da mevcuttu. Yan burun bezleri (Steno bezleri) iyi gelişmişti. Genel olarak yetişkinlerde tek taraflı sinüsün posterosuperior kısmında koronal ve sagittal kesitlerde dört adet etmoturbinat bulunurken, neonatal sıçanlarda iki tane etmoturbinat vardı. İlginç bir şekilde, farede nazofarenksin hemen rostralinde bir septal pencere mevcuttu, ancak yeni doğan sıçanlarda septal pencere görülmedi. Sonuç: Bu çalışma, yeni doğmuş Wistar albino sıçanının ağız ve burun boşluğunun anatomisine ilişkin temel araştırma özelliklerini rapor etmektedir. Verilerimiz, bu organların yapısını tam olarak incelemeyi amaçlayan diğer çalışmalara ışık tutabilir ve yeni doğmuş Wistar albino sıçanlar üzerinde yapılacak daha sonraki deneysel ve morfolojik çalışmalarda faydalı olabilir.

Anahtar Kelimeler

• Kranial bölge
• Steno bezi
• Sıçan

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