HİPERTİROİDİ VE DİYABETİN UTERUSTA VEGF LOKALİZASYONU ÜZERİNE ETKİSİ

Abstract

Hipertiroidi ve diyabet birbirleri ile ilişkili olabilen endokrin sistem hastalıklardır. Çalışmamızda, deneysel diyabet ve deneysel hipertiroidi oluşturulan ratlardan alınan uterus dokusu üzerinde VEGF ekspresyonun karşılıklı değerlendirilmesi amaçlanmıştır. Çalışmada ağırlıkları yaklaşık 200-220 gram ağırlığında 8-10 haftalık Wistar Albino cinsi erişkin dişi rat kullanıldı. Kontrol, diyabetik ve hipertiroidi olmak üzere üç gruba ayrılan hayvanlar östrus siklusuna göre; östrüs dönemlerinde alındılar. Sıçanlara, deneysel diyabet oluşturmak için 60 mg/kg derişimlerde hazırlanan streptozotosin, intraperitonal yoldan enjekte edildikten 30 gün sonrasında uterus dokuları çıkarıldı. Hayvanlarda hipertiroidi oluşturmak için 21 gün süre ile 250 mcg/kg/gün olmak üzere subkutan enjeksiyon yolu ile tiroksin (L-tiroksin) hormonu enjekte edildi. Doku örnekleri %10 tamponlanmış nötral formalin çözeltisinde tespit edildikten sonra parafin bloklardan 3 µm kalınlığında kesitler alındı. Alınan doku uterus örneklerine VEGF lokalizasyonun belirlenebilmesi için immünohistokimyasal yöntemler uygulandı. Elde edilen dokular karşılaştırılarak değerlendirildi ve uygun alanlardan fotoğraflar çekildi. Kontrol ve deney grupları karşılıklı değerlendirildiğinde diyabetik grupta yer alan uterus dokusunda özellikle, epitel ve stroma alanlarında VEGF lokalizasyonun azalmış olduğu dikkat çekti. Hipertiroidi olan grupta da benzer şekilde kontrol grubundaki VEGF lokalizasyonun deney grubunda azalmış olduğu belirlendi. Dolayısıyla, endokrin hastalıklar olan diyabet ve hipertiroidinin VEGF ekspresyonu üzerinde etkili olduğu ve ekspresyon düzeyinin azalması yönünde katkı sağladığı söylenebilir.

Keywords

• Hipertiroidi
• Uterus
• VEGF
• Diyabet

EFFECT OF HYPERTHYROIDISM AND DIABETES ON VEGF LOCALIZATION IN UTERUS

Abstract

Hyperthyroidism and diabetes are endocrine system diseases that can be associated with each other. In our study, we aimed to evaluate VEGF expression on uterine tissue taken from experimentally diabetic and experimentally hyperthyroid rats. In the study, 8-10 week old Wistar Albino adult female rats weighing approximately 200-220 grams were used. The animals were divided into three groups as control, diabetic and hyperthyroid, and were taken during the estrous period according to the estrous cycle. In order to create experimental diabetes, streptozotocin prepared at a concentration of 60 mg/kg was injected intraperitoneally into the rats and uterine tissues were removed 30 days later. Thyroxine (L-thyroxine) hormone was injected subcutaneously for 21 days at a dose of 250 mcg/kg/day to create hyperthyroidism in the animals. After the tissue samples were fixed in 10% buffered neutral formalin solution, 3 µm thick sections were taken from paraffin blocks. Immunohistochemical methods were applied to the tissue samples taken to determine VEGF localization. The tissues obtained were compared and evaluated, and photographs were taken from appropriate areas. When the control and experimental groups were evaluated, it was noted that VEGF localization was reduced in the uterine tissue in the diabetic group, especially in the epithelial and stromal areas. Similarly, in the hyperthyroid group, VEGF localization was reduced in the control group and in the experimental group. Therefore, it can be said that diabetes and hyperthyroidism, which are endocrine diseases, have an effect on VEGF expression and contribute to the decrease in expression levels.

Keywords

• Hyperthyroidism
• Uterus
• VEGF
• Diabetes

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