IMMUNOHISTOCHEMICAL EVALUATION, MOLECULAR INVESTIGATION AND DIFFERENTIAL DIAGNOSIS OF A NOVEL ADIPOCYTOKINE CHEMERİN IN OVARIAN AND UTERINE CANCERS

Hayriye Canik Yaşar; Fatma Zeynep Kırlangıç; Aysun Tekeli Taşkömür; Sultan Çalışkan

doi:10.55895/sshs.1688216

IMMUNOHISTOCHEMICAL EVALUATION, MOLECULAR INVESTIGATION AND DIFFERENTIAL DIAGNOSIS OF A NOVEL ADIPOCYTOKINE CHEMERİN IN OVARIAN AND UTERINE CANCERS

Abstract

Patients diagnosed with the following histological types of carcinoma were included in the study: ovarian serous carcinoma (n=16), ovarian endometrioid carcinoma (n=12), ovarian mucinous carcinoma (n=11), uterine serous carcinoma (n=12) and uterine endometrioid carcinoma (n=15). The study was conducted between 2014 and 2023. The study is based on the comparison of two groups of patients with differing levels of organ involvement. Following the extraction of serum from blood samples, the samples were stored in a temperature of -20°C. The measurement of serum chemerin levels was conducted by means of the ELISA method. In the immunohistochemical study, the sections obtained from the tissues in paraffin blocks were stained for chimerine with the Leica Bond max closed system automatic staining device and Bond polymer refine detection (DS9800, Buffalo Grove, United States) DAB compatible kit. In the immunohistochemical study, the presence or absence of chimerin expression in tumour cells was examined. In the group where such expression was observed, the percentage of staining in the cells was calculated. Furthermore, clinical, laboratory and radiological findings were obtained from the hospital system.

Keywords

References

Ahn, K. Y., Lee, M. K., Kim, D. I., Park, J., Min, J., In Yang, H., ... & Jeon, J. Y. (2016). Cardiopulmonary fitness, adiponectin, chemerin associated fasting insulin level in colorectal cancer patients. Supportive Care in Cancer, 24, 2927-2935.
Barbe, A., Bongrani, A., Mellouk, N., Estienne, A., Kurowska, P., Grandhaye, J., ... & Dupont, J. (2019). Mechanisms of adiponectin action in fertility: an overview from gametogenesis to gestation in humans and animal models in normal and pathological conditions. International journal of molecular sciences, 20(7), 1526.
Barnea, G., Strapps, W., Herrada, G., Berman, Y., Ong, J., Kloss, B., ... & Lee, K. J. (2008). The genetic design of signaling cascades to record receptor activation. Proceedings of the National Academy of Sciences, 105(1), 64-69.
Bozaoglu, K., Bolton, K., McMillan, J., Zimmet, P., Jowett, J., Collier, G., ... & Segal, D. (2007). Chemerin is a novel adipokine associated with obesity and metabolic syndrome. Endocrinology, 148(10), 4687-4694.
Carlino, C., Trotta, E., Stabile, H., Morrone, S., Bulla, R., Soriani, A., ... & Gismondi, A. (2012). Chemerin regulates NK cell accumulation and endothelial cell morphogenesis in the decidua during early pregnancy. The Journal of Clinical Endocrinology & Metabolism, 97(10), 3603-3612.
Fa, T. (2003). Pathology and genetics. Tumours of the breast and female genital organs. Peritoneal tumours, 197-202.
Goralski, K. B., McCarthy, T. C., Hanniman, E. A., Zabel, B. A., Butcher, E. C., Parlee, S. D. & Sinal, C. J. (2007). Chemerin, a novel adipokine that regulates adipogenesis and adipocyte metabolism. Journal of Biological Chemistry, 282(38), 28175-28188.
Juge-Aubry, C.E., E. Henrichot, and C.A. (2005). Meier, Adipose tissue: a regulator of inflammation. Best Pract Res Clin Endocrinol Metab. 19(4): p. 547-66.

Luo, L. Liu, M. (2016). Adipose tissue in control of metabolism. J. Endocrinol. 231, R77--R99.
Maringe, C., Walters, S., Butler, J., Coleman, M. P., Hacker, N., Hanna, L., ... & Rachet, B. (2012). Stage at diagnosis and ovarian cancer survival: evidence from the International Cancer Benchmarking Partnership. Gynecologic oncology, 127(1), 75-82.
Mattern, A. Zellmann, T. Beck-Sickinger, A.G. (2014). Processing, signaling, and physiological function of chemerin. IUBMB Life. 66, 19-26.
Menon, U., Gentry-Maharaj, A., Burnell, M., Singh, N., Ryan, A., Karpinskyj, C., ... & Parmar, M. (2021). Ovarian cancer population screening and mortality after long-term follow-up in the UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS): a randomised controlled trial. The Lancet, 397(10290), 2182-2193.
Moore, K.N., Fader, A.N. (2011). Uterine papillary serous carcinoma. Clin Obstet Gynecol. 54(2):278-291.
Nucci R, Oliva. E 2009. Working together to grow libraries in developing countries. Churchill livingstone elsevier. Published online
Ouchi, N., Parker, J. L., Lugus, J. J., & Walsh, K. (2011). Adipokines in inflammation and metabolic disease. Nature reviews immunology, 11(2), 85-97..
Pachynski, R. K., Zabel, B. A., Kohrt, H. E., Tejeda, N. M., Monnier, J., Swanson, C. D., ... & Butcher, E. C. (2012). The chemoattractant chemerin suppresses melanoma by recruiting natural killer cell antitumor defenses. Journal of Experimental Medicine, 209(8), 1427-1435.
Roh, S. G., Song, S. H., Choi, K. C., Katoh, K., Wittamer, V., Parmentier, M., & Sasaki, S. I. (2007). Chemerin—a new adipokine that modulates adipogenesis via its own receptor. Biochemical and biophysical research communications, 362(4), 1013-1018.
Rourke, J. L., Dranse, H. J., & Sinal, C. J. (2013). Towards an integrative approach to understanding the role of chemerin in human health and disease. Obesity Reviews, 14(3), 245-262. Scheja, L., & Heeren, J. (2019). The endocrine function of adipose tissues in health and cardiometabolic disease. Nature reviews endocrinology, 15(9), 507-524.
Schneider, J. E. (2004). Energy balance and reproduction. Physiology & behavior, 81(2), 289-317.
Skrzeczyńska-Moncznik, J., Stefańska, A., Zabel, B., Kapińska-Mrowiecka, M., Butcher, E., & Cichy, J. (2009). Chemerin and the recruitment of NK cells to diseased skin. Acta Biochimica Polonica, 56(2), 355-360.
Stewart, C., Ralyea, C., & Lockwood, S. (2019, April). Ovarian cancer: an integrated review. In Seminars in oncology nursing (Vol. 35, No. 2, pp. 151-156). WB Saunders.
Teng, F., Cui, G., Qian, L., & Zhao, L. (2023). Changes of T lymphocyte subsets in peripheral blood of patients with intermediate and advanced cervical cancer before and after nimotuzumab combined with chemoradiotherapy. International Archives of Allergy and Immunology, 184(1), 85-97.
Wang, L., Yang, T., Ding, Y., Zhong, Y., Yu, L., & Peng, M. (2015). Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia. Endocrine, 48, 299-308.
Zaitseva, M., Vollenhoven, B. J., & Rogers, P. A. (2008). Retinoids regulate genes involved in retinoic acid synthesis and transport in human myometrial and fibroid smooth muscle cells. Human reproduction, 23(5), 1076-1086.
Zhang, L., Kwan, S. Y., Wong, K. K., Soliman, P. T., Lu, K. H., & Mok, S. C. (2020). Pathogenesis and clinical management of uterine serous carcinoma. Cancers, 12(3), 686.

Details

Primary Language

English

Subjects

Pathology

Journal Section

Research Article

Authors

Hayriye Canik Yaşar ^*
0000-0003-3633-8735
Türkiye

Fatma Zeynep Kırlangıç
0000-0003-2468-8375
Türkiye

Aysun Tekeli Taşkömür
0000-0001-7839-6963
Türkiye

Sultan Çalışkan
0000-0003-3703-8427
Türkiye

Publication Date

April 30, 2025

Submission Date

March 12, 2025

Acceptance Date

March 16, 2025

Published in Issue

Year 2025 Volume: 7 Number: 1

DOI

https://doi.org/10.55895/sshs.1688216

IZ

https://izlik.org/JA76XK46MH

Cite

RIS / Bibtex

APA

Canik Yaşar, H., Kırlangıç, F. Z., Tekeli Taşkömür, A., & Çalışkan, S. (2025). IMMUNOHISTOCHEMICAL EVALUATION, MOLECULAR INVESTIGATION AND DIFFERENTIAL DIAGNOSIS OF A NOVEL ADIPOCYTOKINE CHEMERİN IN OVARIAN AND UTERINE CANCERS. Sabuncuoglu Serefeddin Health Sciences, 7(1), 22-28. https://doi.org/10.55895/sshs.1688216

AMA

1.Canik Yaşar H, Kırlangıç FZ, Tekeli Taşkömür A, Çalışkan S. IMMUNOHISTOCHEMICAL EVALUATION, MOLECULAR INVESTIGATION AND DIFFERENTIAL DIAGNOSIS OF A NOVEL ADIPOCYTOKINE CHEMERİN IN OVARIAN AND UTERINE CANCERS. SSHS. 2025;7(1):22-28. doi:10.55895/sshs.1688216

Chicago

Canik Yaşar, Hayriye, Fatma Zeynep Kırlangıç, Aysun Tekeli Taşkömür, and Sultan Çalışkan. 2025. “IMMUNOHISTOCHEMICAL EVALUATION, MOLECULAR INVESTIGATION AND DIFFERENTIAL DIAGNOSIS OF A NOVEL ADIPOCYTOKINE CHEMERİN IN OVARIAN AND UTERINE CANCERS”. Sabuncuoglu Serefeddin Health Sciences 7 (1): 22-28. https://doi.org/10.55895/sshs.1688216.

EndNote

Canik Yaşar H, Kırlangıç FZ, Tekeli Taşkömür A, Çalışkan S (April 1, 2025) IMMUNOHISTOCHEMICAL EVALUATION, MOLECULAR INVESTIGATION AND DIFFERENTIAL DIAGNOSIS OF A NOVEL ADIPOCYTOKINE CHEMERİN IN OVARIAN AND UTERINE CANCERS. Sabuncuoglu Serefeddin Health Sciences 7 1 22–28.

IEEE

[1]H. Canik Yaşar, F. Z. Kırlangıç, A. Tekeli Taşkömür, and S. Çalışkan, “IMMUNOHISTOCHEMICAL EVALUATION, MOLECULAR INVESTIGATION AND DIFFERENTIAL DIAGNOSIS OF A NOVEL ADIPOCYTOKINE CHEMERİN IN OVARIAN AND UTERINE CANCERS”, SSHS, vol. 7, no. 1, pp. 22–28, Apr. 2025, doi: 10.55895/sshs.1688216.

ISNAD

Canik Yaşar, Hayriye - Kırlangıç, Fatma Zeynep - Tekeli Taşkömür, Aysun - Çalışkan, Sultan. “IMMUNOHISTOCHEMICAL EVALUATION, MOLECULAR INVESTIGATION AND DIFFERENTIAL DIAGNOSIS OF A NOVEL ADIPOCYTOKINE CHEMERİN IN OVARIAN AND UTERINE CANCERS”. Sabuncuoglu Serefeddin Health Sciences 7/1 (April 1, 2025): 22-28. https://doi.org/10.55895/sshs.1688216.

JAMA

1.Canik Yaşar H, Kırlangıç FZ, Tekeli Taşkömür A, Çalışkan S. IMMUNOHISTOCHEMICAL EVALUATION, MOLECULAR INVESTIGATION AND DIFFERENTIAL DIAGNOSIS OF A NOVEL ADIPOCYTOKINE CHEMERİN IN OVARIAN AND UTERINE CANCERS. SSHS. 2025;7:22–28.

MLA

Canik Yaşar, Hayriye, et al. “IMMUNOHISTOCHEMICAL EVALUATION, MOLECULAR INVESTIGATION AND DIFFERENTIAL DIAGNOSIS OF A NOVEL ADIPOCYTOKINE CHEMERİN IN OVARIAN AND UTERINE CANCERS”. Sabuncuoglu Serefeddin Health Sciences, vol. 7, no. 1, Apr. 2025, pp. 22-28, doi:10.55895/sshs.1688216.

Vancouver

1.Hayriye Canik Yaşar, Fatma Zeynep Kırlangıç, Aysun Tekeli Taşkömür, Sultan Çalışkan. IMMUNOHISTOCHEMICAL EVALUATION, MOLECULAR INVESTIGATION AND DIFFERENTIAL DIAGNOSIS OF A NOVEL ADIPOCYTOKINE CHEMERİN IN OVARIAN AND UTERINE CANCERS. SSHS. 2025 Apr. 1;7(1):22-8. doi:10.55895/sshs.1688216