INVESTIGATION OF WASP GENE EXPRESSION IN PERIODONTITIS

Year 2022, Volume: 4 Issue: 2, 17 - 28, 31.08.2022

Kübra Şahin Ömer Ateş

https://doi.org/10.55895/sshs.1149632

Abstract

Periodontitis, mikroorganizmalar ve konak bağışıklık sistemindeki dengesizlik sonucu ortaya çıkan, periodonsiyumda yıkıma ve kemik kaybına neden olan ve aynı zamanda sistemik sağlığı tehdit eden kronik inflamatuar bir hastalıktır. İnflamatuar ve immün yolların düzensizliği kronik inflamasyona, doku yıkımına ve hastalıklara neden olur. Bu nedenle doğal ve adaptif immün defektler, periodontitis gibi immün aracılı inflamatuar hastalıklarda önemli bir rol oynamaktadır. İmmün hücrelerin doğru ve koordineli hareketi, göç, hücre aktivasyonu, antijen alımı ve tanınması gibi birçok süreçte rol oynayan aktin hücre iskeletinin düzenlenmesine bağlıdır. Çalışmalar, önemli bir aktin hücre iskeleti düzenleyicisi olan Wiskott-Aldrich sendromu proteini (WASP) ekspresyon seviyesindeki değişikliklerin, immün ve inflamatuar yanıt oluşumunda kusurlara neden olduğunu göstermiştir. Çalışmamızda periodontitis ve sağlıklı dişeti dokusunda WASP gen ekspresyonunun araştırılması ve periodontitis ile olası ilişkisinin ortaya konması amaçlanmıştır. Bu doğrultuda çalışmaya 10 gönüllü dahil edildi ve her hastadan sağlıklı dişeti ve periodontitis dokusu alındı. Gen ekspresyon seviyeleri SYBR Green temelli PCR ile belirlendi. Sonuçlarımıza göre periodontitis dokularında WASP mRNA düzeyleri sağlıklı dişeti dokusuna göre istatistiksel olarak anlamlı derecede yüksek bulundu (p<0.05).
WASP'ın periodontitis gelişiminde etkili olabileceğini düşündüren veriler elde edilmiştir. Bununla birlikte, WASP’ın periodontitisteki rolünü tam olarak anlamak için büyük bir örneklem grubuna sahip daha fazla araştırmaya ihtiyaç vardır.

Keywords

cytoskeleton, Inflammation, Immunity, Periodontitis, WASP

Supporting Institution

This study was encouraged by Tokat Gaziosmanpasa University Scientific Research Projects

Project Number

2021/56

Thanks

The authors are grateful to Esengül SEN for collecting the samples

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