In Vitro Haploidy Techniques in Ornamental Plants

Year 2017, Volume: 10 Issue: 1, 1 - 6, 01.06.2017

Mehmet Tütüncü Tolga İzgü Başar Sevindik Yesim Yalcın Mendi

Abstract

The ornamental plant industry is very dynamic and diverse sector having nearly $90 billion production value around the world. Plant breeders develop a great number of new cultivars each year in order to increase production amount and supply market demands of ornamental plants. Various methods have been used in plant breeding, but conventional methods such as selection and crossing are greatly time-consuming processes. In recent years, haploidization techniques based on plant tissue culture are commonly used in ornamental plant breeding in order to overcome disadvantages of conventional methods. The haploid plants described as chromosome numbers in somatic cell of the plant are as many as its in gametic cells. These plants can be formed spontaneously in nature via developing egg cell or synergids into an embryo without fusion of gametic cells or can be obtained using different techniques. At present, in situ and in vitro haploid/double haploid techniques have been successfully utilized in many plants to accelerate breeding process. In vitro haploidy techniques that are gynogenesis (ovule, ovary, flower bud culture) and androgenesis (anther and microspore culture) presented in the present review and some of the successful studies on in vitro haploidy induction in ornamental plants were illustrated.

Keywords

Androgenesis, Breeding, Floriculture, Gynogenesis

References

• Rabobank, “World Floriculture Map 2016: Equator Countries Gathering Speed,” 2016. https://research.rabobank.com/far/en/sectors/regional-foodagri/World_floriculture_ map_2016.html
• S. Ellialtıoğlu, N. Sarı, K. Abak, “Haploid plant production, In: Plant biotechnology I – tissue culture and applications (In Turkish),” M. Babaoğlu, E. Gürel, S. Özcan (Eds.) , 2nd Edition, Selcuk University Foundation Publications, Konya, 137-189, 2001.
• J. Murovec, B. Bohanec, “Haploid and doubled haploids in plant breeding,” Y. I. Abdurakhmonov (Ed.) Plant breeding, Intech, 87-106, 2012.
• Ü. Emiroğlu, “Anther culture in Tobacco cultivars (In Turkish),” Aegean Agricultural Research Institute, 17 (41), 12-18, 1980.
• K. Abak, “Utilizing anther cultures in pepper breeding, Plant breeding symposium proceedings book (In Turkish),” TÜBİTAK TOAG, 59-66, 1993.
• G. Çağlar, K. Abak, “Obtaining haploid embryo and plant in different cucumber cultivars through irritated pollens (In Turkish),” II. National Horticulture Congress of Turkey, Vol. II, 159-162, 1999.
• J. M. Dunwell, “Haploids in flowering plants: origins and exploitation,” Plant Biotechnol. J., 8, 377-424, 2010.
• V. K. Mishra, R. Goswami, “Haploid productions in higher plant,” IJCBS review paper Vol. 1, 25-44, 2014.
• A. Taji, and R. Williams, “Use of in vitro breeding strategies in the development of Australian native plants,” Acta Hortic. 683, pp. 87-94, 2005. A. F. Blakeslee, J. Belling, M. E. Farnham, A. D. Bergner, “A haploid mutant in the Jimson weed, Datura stramonium,” Science, Vol. 55, pp. 646-647, 1922.
• B. P. Forster, E. Heberle-Bors, K. J. Kasha, A. Touraev, “The resurgence of haploids in higher plants,” Trends Plant Sci., Vol. 12, No. 8, pp. 368-375, 2007.
• J. M. Dunwell, “Haploids in flowering plants: origins and exploitation,” Plant Biotechnol. J., 8: pp. 377-424, 2010.
• Ö. E. Yılmaz, “Haploid plant obtaining in summer squash (Cucurbita pepo L.) through ovary culture (In Turkish),” Msc Thesis, Kahramanmaraş University, Institute of Natural and Applied Science, Kahramanmaraş. 2005.
• T. Ishii, R. Karimi-Ashtiyani, A. Houben, “Haploidization via chromosome elimination: means and mechanisms,” Annu. Rev. Plant Biol., 67, pp. 421-438, 2016.
• S. M. Reed, “Haploid culture In: Plant development and biotechnology,” R. N. Trigiano, D. J. Gray (Eds.), CRC press LLC, NW, USA pp. 225-234. 2005.
• J. A. Teixeria da Silva, B. Winarto, J. Dobránszki, S. Zeng, “Anther culture of Anthurium: a review,” Acta Physiol. Plant., 37 (8), 173, 2015.
• Ş. M. Doğru, A. Çilingir, E. Kurtar, A. Balkaya, “Using of haploid and double haploid technique in breeding of cabbage group vegetables (In Turkish),”TURKTOB J., 5 (20), pp. 30-33, 2016.
• S. K. Datta, “Plant regeneration by pollen embryogenesis from cultured whole spikes of barley (Hordeum vulgare),” Theor. Appl. Genet., 74, pp. 121-124, 1987.
• S. K. Datta and G. Wenzel, “Isolated microspore derived plant formation via embryogenesis in Triticum aestivum. Plant Sci., 48, pp. 49-54, 1987.
• S. K. Datta and I. Potrykus, “Direct pollen embryogenesis in cereals,” Experentia, 44, 43. 1998, N. Sunderland and J. M. Dunwell, “Pathways in pollen embryogenesis, In: Tissue culture and Plant Science,” H. E. Street, Academic Press, New York, pp. 141-167, 1974.
• A. Jähne and H. Lörz, “Cereal microspore culture,” Plant Sci., 109, pp. 1-12, 1995.
• S. K. Datta, “Androgenic haploids: Factors controlling development and its application in crop improvement,” Curr. Sci., Vol. 89, No. 11 pp. 1870-1878, 2005.
• M. A. Germana, “Anther culture for haploid and doubled haploid production,” Plant Cell Tiss. Organ Cult., 104, pp. 283-300, 2011.
• M. Y. Zheng, “Microspore Culture in Wheat (Triticum Aestivum)-Doubled Haploid Production via induced Embryogenesis,” Plant Cell. Tiss. and Organ Cult., 73, pp. 213-230, 2003.
• A. M. R. Ferrie, K. L. Caswell, “Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production,” Plant Cell Tiss. Organ Cult., 104, pp. 301-309, 2011.
• S. M. S. Islam, I. Ara, N. Tuteja, S. Subramaniam, “Efficient microspore isolation methods for high yield embryoids and regeneration in rice (Oryza sativa L.),” World Aca Sci, Engi Tech (WASET), Inter. J. Biol. Sci. Engi., 7 (12), pp. 891-96, 2013.
• O. S. Ayed, J. De Buyser, E. Picard, Y. Trifa and H.S. Amara, “Effect of pre-treatment on isolated microspores culture ability in durum wheat (Triticum turgidum subsp. durum Desf.),” J. Plant Breed. Crop Sci., 2, pp. 30-38, 2010.
• E. Dubas, M. Wedzony, B. Petrovska, J. Salaj, I. Zur, “Cell structural reorganization during induction of androgenesis in isolated microspore cultures of Triticale ×Triticosecale Wittm.),” Acta Biol. Cracov., Ser. Bot., 52 (1), pp. 73-86, 2010.
• S. V. Bergen and M. Wang, “Microspore regeneration as a tool for plant breeding: Barley microspore regeneration as a model system,” Acta Hortic., 572, pp. 51-57, 2002.
• S. B. Babbar, P. K. Agarwal, S. Sahay, S. S. Bhojwani, “Isolated microspore culture of Brassica: an experimental tool for developmental studies and crop improvement” Indian J. Biotechnol., Vol. 3, pp. 185-202, 2004.
• F. L. Olsen, “Isolation and cultivation of embryogenic microspores from barley (Hordeum vulgave L.),” Hereditas 115, pp. 255-266, 1991.
• B. Obert, Z. Žáčková, J. Šamaj, A. Preťová, “Doubled haploid production in Flax (Linum usitatissimum L.), Biotechnol. Adv., 27, pp. 371-375, 2009. [34] R. H. Smith, M. Kamp, R. S. Davies, “Reduced plant size of haploid African plant violets. In Vitro, 17 (5), pp. 385-387, 1981.
• A. Razdan, M. K. Razdan, M. V. Rajam, S. N. Raina, “Efficient protocol for in vitro production of androgenic haploids of Phlox drummondii,” Plant Cell Tiss. Organ Cult., 95, pp. 245-250, 2008.
• D. S. Han, Y. Niimi, M. Nakano, “Production of doubled haploid plants through colchicine treatment of anther-derived haploid calli in the Asiatic Hybrid Lily ‘Connecticut King’,” J. Japan. Soc. Hort. Sci., 68 (5), pp. 979-983, 1999.
• X. Zhang, Q. Wu, X. Li, S. Zheng, S. Wang, L. Guo, L. Zhang, J. B. M. Custers, “Haploid plant production in Zantedeschia aethiopica ‘Hong Gan’ using anther culture,” Sci. Hortic., 129, pp. 335-342, 2011.
• S. Wang, X. Li, L. Yang, H. Wu, S. Zheng, X. Zhang, L. Zhang, “Microspore culture of Zantedeschia aethiopica: The role of monosaccharides in sporophytic development,” Afr. J. Biotechnol., 10 (50), pp. 10287-10292, 2011.
• R. K. Khandakar, Y. U. Jie, M. I. N. Sun-Kyung, W. O. N. Mi-Kyoung, H. G. Choi, P. A. R. K. Ha-Seung, K. I. M Tae-Sung, “Regeneration of haploid plantlet through anther culture of Chrysanthemum,” Not. Bot. Horti Agrobot. Cluj-Napoca, 42 (2), 482, 2014.
• A. M. Arzate-Fernandez, T. Nakazaki, H. Yamagatab, T. Tanisaka, “Production of doubled-haploid plants from Lilium longiflorum Thunb. anther culture, Plant Sci., 123, pp. 179-187, 1997.
• H. Doi, R. Takahashi, T. Hikage, “Embryogenesis and doubled haploid production from anther culture in gentian (Gentiana triflora),” Plant Cell Tiss. Organ Cult., 102, 27-33, 2010.
• M. M. A. Arzate-Fernandez and A. C. Hildebrandt, “Origin of androgenetic callus and haploid geranium plants, Can. J. But., 51, pp. 2107-2109, 1973.
• E. Ari, H. Bedir, S. Yildirim, T. Yildirim, “Androgenic responses of 64 ornamental pepper (Capsicum annuum L.) genotypes to shed-microspore culture in the autumn season,” Turk J. Biol., 40, pp. 706-717, 2016.
• E. Ari, T. Yildirim, N. Mutlu, S. Büyükalaca, Ü. Gökmen, E. Akman, “Comparison of different androgenesis protocols for doubled haploid plant production in ornamental pepper (Capsicum annuum L.),” Turk J. Biol., 40, pp. 944-954, 2016.
• R. S. Sangwan and B. Norreel, “Induction of plants from pollen grains of Petunia cultured in vitro,” Nature, 175, pp. 222-224, 1975.
• M. Laura, G. Safaverdi, A. Allavena, “Androgenetic plants of Anemone coronaria derived through anther culture,” Plant Breed., 125, pp. 629-634, 2006.
• R. W. Van den Bulk, H. P. J. De Vries-Van Hulten, J. B. M. Custers, J. J. M. Dons, “Induction of embryogenesis in isolated microspores of tulip,” Plant Sci., 104 (1), pp. 101-111, 1994.
• G. L. Sita, “Gynogenic haploids in vitro, In: In vitro haploid production in higher plants,” S. M. Jain, S. K. Sopory, R. E. Veilleux (Eds.), Springer Science+Business Media B.V., Netherlands, pp. 175-193, 1997.
• A. M. R. Ferrie, “Doubled haploidy as a tool in ornamental breeding,” Acta Hortic., 953, pp. 167-171, 2012.
• K. R. Shivanna, “Pollen biology and biotechnology,” Science Publishers, pp. 316, 2003.
• H. Doi, S. Yokoi, T. Hikage, M. Nishihara, K. I. Tsutsumi, Y. Takahata, “Gynogenesis in gentians (Gentiana triflora, G. scabra): production of haploids and doubled haploids,” Plant Cell Rep., 30 (6), pp. 1099-1106, 2011.
• S. K. Mukhambetzhanov, “Culture of nonfertilized female gametophytes in vitro,” Plant Cell Tiss. Organ Cult.,48, pp.111–119, 1997.
• B. Bohanec, “Doubled haploid via gynogenesis. In: Advances in haploid production in higher plants,” A. Touraev, B. P. Forster, S. M. Jain (Eds). Springer, Berlin, pp. 35-46, 2009.
• M. Sitbon, “Production of haploid Gerbera jamesonii plants by in vitro culture of unfertilized ovules,” Agronomie, EDP Sciences, 1 (9), pp. 807-812, 1981.
• S. K. Sopory and M. Munshi, “Anther culture. In: In vitro haploid production in higher plants,” J. S. Mohan, S. K. Sopory, R. E. Veilleux (Eds.), Kluwer Academic Publishers, Dordrecht, Vol 1, pp. 145-176, 1996.
• J. F. Chen, L. Cui, A. A. Malik, K. G. Mbira, “In vitro haploid and dihaploid production via unfertilized ovule culture,” Plant Cell Tiss. Organ Cult., 104, pp. 311-319, 2011.
• R. Pathirana, T. Frew, D. Hedderley, G. Timmerman-Vaughan, E. R. Morgan, “Haploid and doubled haploid plants from developing male and female gametes of Gentiana triflora,” Plant Cell Rep., 30, pp. 1055-1065, 2011.
• H. Doi, N. Hoshi, E. Yamada, S. Yokoi, M. Nishihara, T. Hikage, Y. Takahata, “Efficient haploid and doubled haploid production from unfertilized ovule culture of gentians (Gentiana spp.),” Breed. Sci., 63 (4), pp. 400-406, 2013.
• M. Ahmim, J. Vieth, “Production de plantes haploides de Gerbera jamesonii par culture in vitro d’ovules,” Can. J .Bot., 64, pp. 2355-2357, 1986.
• K. Miyoshi, and N. Asakura, “Callus induction, regeneration of haploid plants and chromosome doubling in ovule cultures of pot gerbera (Gerbera jamesonii),” Plant Cell Rep., 16 (1), pp. 1-5, 1996.
• J. W. DeVerna and G. B. Collins, “Maternal haploids of Petunia axillaris (Lam.) BSP via culture of placenta attached ovules,” Theor. Appl. Genet., 69 (2), pp. 187-192, 1984. T. Eeckhaut, S. Werbrouck, J. Dendauw, E. Van Bockstaele, P. Debergh, “Induction of homozygous Spathiphyllum wallisii genotypes through gynogenesis,” Plant Cell, Tiss. and Organ Cult., 67 (2), pp. 181-189, 2001.
• A. K. Sharma and A. Sharma, “Chromosome techniques—A manual,” Harwood Academic Publisher, Switzerland, p. 368, 1994.
• B. Winarto, N. A. Mattjik, J. A. Teixeira da Silva, A. Purwito, B. Marwoto, “Ploidy screening of anthurium (Anthurium andreanum Linden ex André) regenerants derived from anther culture,” Sci. Hortic., 127 (1), pp. 86-90, 2010.
• N. Sari, K. Abak, M. Pitrat, “Comparison of ploidy level screening methods in watermelon: Citrullus lanatus (Thunb.) Matsum. and Nakai,” Sci. Hort., 82 (3-4), pp. 265-277, 1999.
• S. S. Yudanova, E. I. Maletskaya, S. I. Maletskii, “Variability of chloroplast number in populations of stomata guard cells in sugar beet Beta vulgaris L.,” Russ. J. Genet., 38 (1), pp. 58-63, 2002.
• I. Ho, Y. Wan, J. M. Widholm, A. L. Rayburn, “The use of stomatal chloroplast number for rapid determination of ploidy level in maize,” Plant Breed., 105 (3), pp. 203-210, 2006.
• S. L. Beck, R.W. Dunlop, A. Fossey, “Stomatal length and frequency as measure of ploidy level in black wattle Acacia mearnsii (de Wild),” Bot. J. Linn. Soc., 141, pp. 177-181, 2003.
• K. Van Laere, S. C. França, H. Vansteenkiste, J. Van Huylenbroeck, K. Steppe, M. C. Van Labeke, “Influence of ploidy level on morphology, growth and drought susceptibility in Spathiphyllum wallisii,” Acta Physiol. Plant., 33 (4), pp. 1149-1156, 2011.
• A. Naiki, H. Nagamasu, “Correlation between distyly and ploidy level in Damnacanthus (Rubiaceae),” Am. J. Bot. 91, pp. 664-671, 2004. C. Singsit, P. Ozias-Akins, “Rapid estimation of ploidy levels in in vitro regenerated interspecific Arachis hybrids and fertile triploids,” Euphytica 64, pp. 183-188, 1992. J. Dolezel, J. Bartos, “Plant DNA flow cytometry and estimation of nuclear genome size,” Ann. Bot., 95, pp. 99-110, 2005.
• J. Dolezel, J. Greilhuber, J. Suda, “Estimation of nuclear DNA content in plants using flow cytometry,” Nat. Protoc. 2, pp. 2233-2244, 2007.
• M. Á. Agulló-Antón, E. Olmos, J. M. Pérez-Pérez, M. Acosta, “Evaluation of ploidy level and endoreduplication in carnation (Dianthus spp.),” Plant Sci., 201, pp. 1-11, 2013.
• H. Ishizaka, T. Oku, K. I. Mishiba, M. Mii, “Identification of Ploidy Level of Cyclamen rohlfsianum plants through flow cytometric and cytological analysis of C. rohlfsianum, C. purpurascens and their hybrid. Cytologia,” Caryologia, 74 (4), pp. 457-465, 2009.
• D. J. Roberts and D. J. Werner, “Genome size and ploidy levels of Cercis (Redbud) species, cultivars, and botanical varieties,” Hort. Sci., 51 (4), pp. 330-33, 2016.
• P. J. Zale and P. Jourdan, “Genome size and ploidy of Phlox paniculata and related germplasm in subsections Paniculatae and Phlox,” J. Am. Soc. Hortic. Sci., 140 (5), pp. 436-448, 2015.
• S. Plaschil, H. Budahn, O. Schrader, K. Olbricht, M. Wiedemann, C. Hofmann, “Tetraploid male fertile Pelargonium crispum hybrids and their use in interspecific hybridization,” In XXV International EUCARPIA Symposium Section Ornamentals: Crossing Borders, 1087 pp. 345-350, 2015.
• G. Besnard, C. Garcia-Verdugo, R. R. De Casas, U. A. Treier, N. Galland, P. Vargas, “Polyploidy in the olive complex (Olea europaea): evidence from flow cytometry and nuclear microsatellite analyses,” Ann. Bot., 101 (1), pp. 25-30, 2008.
• J. M. Segui-Simarro and F. Nuez, “Mini review how microspores transform into haploid embryos: changes associated with embryogenesis induction and microspore derived embryogenesis,” Physiol. Plant. 134, pp. 1-12, 2008.
• T. D. Silva, “Microspore embryogenesis, embryogenesis,” K. Sato (Ed.), InTech, DOI: 10.5772/37039, 2012.
• B. Tuncer, R. Yanmaz, “One of the ways obtaining haploid plants: microspore culture (In Turkish),” Alatarım 6 (2), pp. 1-8, 2007.