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Alıç Meyvesi Liyofilize Ekstraktının Sıçanlarda Karbon Tetraklorür Toksisitesine Karşı Antioksidan ve Koruyucu Rolünün Değerlendirilmesi

Year 2021, Volume: 10 Issue: 2, 42 - 47, 31.12.2021
https://doi.org/10.46810/tdfd.850150

Abstract

Bu makale, karbon tetraklorür (CCl4) toksisitesine karşı alıç (Crataegus orientalis L.) meyve liyofilize ekstrakt (LEH), böbrek ve karaciğer koruyucu potansiyelini ve antioksidan rolünü araştırmayı amaçlamaktadır. Deneysel dizayn; Normal kontrol (NK) CCl4 grubu (0,5 ml / kg CCl4, vücut ağırlığı (wb)), LEH1grubu (100 mg / kg LEH bw), LEH4 grubu (200 mg / kg LEH bw), CCl4+LEH1 grubu (0,5 ml / kg + 100 mg / kg LEH bw) ve CCl4+LEH4 grubu (0,5 ml / kg + 200 mg / kg LEH bw) şeklinde 3 haftalık ekstre muamelesi ile yapılmıştır. Deneysel muamelenin sonunda: LEH'nin böbrek ve karaciğer koruyucu potansiyeli ve antioksidan rolünü, karaciğer ve böbrek hasarı serum biyobelirteçleri, eritrosit, beyin, börek ve karaciğerdeki antioksidan savunma sistemleri bileşenleri (ASSB) ile malondialdehit (MDA) içeriği ölçülerek değerlendirildi. Sonuçlara göre; karaciğer hasarı serum enzim aktiviteleri ve dokuların MDA içeriği CCl4 grubunda NK göre anlamlı olarak daha yüksekti. Bu parametreler, ekstre takviyeli gruplarda CCl4 grubuna kıyasla önemli ölçüde daha düşüktü. Sonuçlara göre, LEH takviyeli diyetinin ASSB'lerdeki dalgalanmaları NK değerlerine doğru parametrelerin çoğunu geri çektiğini göstermektedir. Bu nedenle, CCl4 ile toksisite oluşturulan sıçanlarda alıç bitkisi meyve ekstresinin antioksidan kapasiteye ve karaciğer koruyucu etkilere sahip olabileceği düşünülmektedir.

Supporting Institution

Van Yüzüncü Yıl Üniversitesi

Project Number

FYL-2017-5755

Thanks

VYYÜ Bilimsel Araştırma Projeleri Başkanlığı

References

  • [1] Yu LL, Zhou KK, Parry J. Antioxidant properties of cold pressed black caraway, carrot, cranberry, and hemp seed oils. Food Chem 2005; 91: 723-729.
  • [2] Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M. et al. Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chemi 2005; 9: 621-632.
  • [3] Liu F, Ng TB. Antioxidative and free radical scavenging activities of selected medicinal herbs. Life Science 2000; 66: 725-735.
  • [4] Halliwell B. Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans. Free Radic Res 1996; 25: 57-74.
  • [5] Vendemiale G, Grattagliano I, Altomare E. An update on the role of free radicals and antioxidant defense in human disease. Int J Clin Lab Res 1999; 29: 49-55.
  • [6] Christensen KI. Revision of Crataegus sect. Crataegus and Nothosect. Cratae guineae (Rosaceae-Maloideae) in the Old World. Syst. Bot. Monograms 1992; 35: 1-199.
  • [7] Ljubuncic P, Portnaya I, Cogan U, Azaizeh H, Bomzon A Antioxidant activity of Crataegus aronia aqueous extract used in traditional Arab medicine in Israel. J Ethnopharmacol 2005; 101: 153-161. [8] Baytop T. Treatment with plants in Turkey. Istanbul University Publication No. 3255, Istanbul; 1984.
  • [9] Bahorun T, Trotin F. Antioxidant activities of Crataegus monogyna extracts. Planta Med 1994; 60: 323-326.
  • [10] Bahorun T, Greiser B. Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneimittel-Forsch 1996; 46: 1086-1089.
  • [11] Rakotoarison DA, Greissier B. Antioxidant activities of phenolic extracts from flowers, in vitro callus and cell suspension cultures of Crataegus monogyna. Pharmazie 1997; 52: 60-4. [12] Horoz M, Gok E, Genctoy G, Ozcan T, Olmaz R, Akca M, Kiykim A, Gurses I. Crataegus orientalis Associated Multiorgan Hypersensitivity Reaction and Acute Renal Failure. Internal Medicine 2008; 47: 2039-2042.
  • [13] Arslan R, Bor Z, Bektas N, Meriçli AH, Oztur Y. Antithrombotic effects of ethanol extract of Crataegus orientalis in the carrageenan-induced mice tail thrombosis model. Thromb Res 2011; 127: 210-213.
  • [14] Dalar A, Konczak I. Phenolic contents, antioxidant capacities and inhibitory activities against key metabolic syndrome relevant enzymes of herbal teas from Eastern Anatolia. Ind Crop Prod 2013; 44: 383-390.
  • [15] Kim SH, Cheon HJ, Yun N, Oh ST, Shin E, Shim KS. et al. Protective effect of a mixture of Aloe vera and Silybum marianum against carbon tetrachloride-induced acute hepatotoxicity and liver fibrosis, J Pharmacol Sci 2009; 109(1): 119-27.
  • [16] Celik I, Temur A, Isık I. Hepatoprotective role and antioxidant capacity of pomegranate (Punica granatum L.) flowers infusion against trichloroacetic acid-exposed in rats. Food Chem Toxicol 2009; 47: 145-149.
  • [17] Dogan A, Celik I. Hepatoprotective and antioxidant activities of grape seeds against ethanol-induced oxidative stress in rats. Br J Nutr 2011; 107: 45-51.
  • [18] Yurt B, Celik I. Hepatoprotective effect and antioxidant role of sun, sulphited-dried apricot (Prunus armeniaca L.) and its kernel against ethanol-induced oxidative stress in rats. Food Chem Toxicol 2011; 49(2): 508-513.
  • [19] Jain SK, McVie R, Duett J, Herbst JJ. Erythrocyte membrane lipid peroxidation and glycolylated hemoglobin in diabetes. Diabetes 1989; 38: 1539-1543.
  • [20] Beutler E, Dubon O, Kelly M. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 61: 882-888.
  • [21] Mannervik B, Guthenberg C. Glutathione S-transferase (Human Plasenta). Method Enzymol 1981; 77: 231–235.
  • [22] Paglia DE, Valentine WN. Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70: 158.
  • [23] McCord JM, Fridovich I Superoxide dismutase, Anenzymatic function for erythrocuprein (hemocuprein). J Biol Chem 1969; 244: 6049-6053.
  • [24] Aebi H Catalase, In Methods of Enzymatic Analysis (Bergemeyer, H U.,ed) New York, NY, US: Academic Press: 1974. p. 673-684.
  • [25] Haagen L, Brock AA new automated method for phenotyping arylesterase (E.C. 3.1.1.2) based upon inhibition of enzymatic hydrolysis of 4-nitrophenyl acetate by phenyl acetate. Eur J Clin Chem Clin Biochem 1992; 30:391-395.
  • [26] Sallie R, Tredger JM, William R. Drugs and the liver. Part I. Testing liver function. Biopharm Drug Disp 1991; 112:251-259.
  • [27] Recknagel RO. A new direction in the study of carbon tetrachloride hepatotoxicity. Life Science 198; 33(5): 401-8.
  • [28] Turkdogan MK, Ozbek H, Yener Z, Tuncer I, Uygan I, Ceylan E. The role of Urtica dioica and Nigella sativa in the prevention of carbon tetrachloride induced hepatotoxicity in rats. Phytother Res 2003; 17: 942-946.
  • [29] Suzek H, Celik I, Dogan A, Yildirim S. (2016) Protective effect and antioxidant role of sweetgum (Liquidambar orientalis) oil against carbon tetrachloride-induced hepatotoxicity and oxidative stress in rats. Pharm Biol 54 (3): 451-457.
  • [30] Suzek H, Celik I, Dogan A. Nephroprotective Hepatoprotective Potential and Antioxidant Role of Carob Pods (Cerotonia siliqua L.) against Carbon Tetrachloride-induced Toxicity in Rats. Ind J Pharm Edu Res 2017; 51(2). 312-320.
  • [31] Chidambara Murthy KN, Rajesha J, Vanitha A, Swamy MM, Ravishankar GA. Protective effect of Dunaliella salina-a marine micro alga, against carbon tetrachloride-induced hepatotoxicity in rats. Hepatol Rese 2005; 33(4):313-9.
  • [32] Freeman BA, Crapo JD. Hyperoxia increases oxygen radical production in rat lung and lung mitochondria. J Biol Chem 1981; 256: 10986-10992.
  • [33] Smith GJ, Litwack G. Roles of ligandin and the glutathione S-transferases in binding steroid metabolites, carcinogens and other compounds. Rev Biochem Toxicol 1980; 2: 1-47.

Evaluation of Antioxidant and Protective Role of Hawthorn Fruit Lyophilized Extract Against Carbon Tetrachloride Toxicity in Rat

Year 2021, Volume: 10 Issue: 2, 42 - 47, 31.12.2021
https://doi.org/10.46810/tdfd.850150

Abstract

This manuscript has aimed to investigate the nephro-hepato protective potential and antioxidant role of lyophilized extract of hawthorn (Crataegus orientalis L) fruit (LEH) against carbon tetrachloride (CCl4) toxicity. The experimental were conducted as; 0.5 ml/kg CCl4, bw), 100 mg/kg LEH bw, 200 mg/kg LEH bw, CCl4 0.5 ml/kg + 100 mg/kg LEH bw and CCl4 0.5 ml/kg + 200 mg/kg LEH bw treated with the extract for 3 weeks. At the end of the experimental treatment, the nephro-hepato protective potential and antioxidant capacity of the LEH was evaluated measuring by liver and kidney damage serum biomarkers, antioxidant defense systems constituents (ADSCs) and malondialdehyde (MDA) content in the erythrocyte, brain, kidney and liver tissues of rats. According to results; liver damage serum enzymes activities and MDA content of the tissues were significantly higher in CCl4 group compared to normal control (NC) whereas; these parameters were significantly lower in extract supplemented groups compared to CCl4 group. According to the resuls, the study results suggest that the LEH supplementations diet restored most of the parameters towards the NC values with fluctuations in the ADSCs. Therefore, it is thought that the extract of hawthorn has antioxidant capacity and hepatoprotective effects against in CCl4-intoxicated rats.

Project Number

FYL-2017-5755

References

  • [1] Yu LL, Zhou KK, Parry J. Antioxidant properties of cold pressed black caraway, carrot, cranberry, and hemp seed oils. Food Chem 2005; 91: 723-729.
  • [2] Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M. et al. Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chemi 2005; 9: 621-632.
  • [3] Liu F, Ng TB. Antioxidative and free radical scavenging activities of selected medicinal herbs. Life Science 2000; 66: 725-735.
  • [4] Halliwell B. Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans. Free Radic Res 1996; 25: 57-74.
  • [5] Vendemiale G, Grattagliano I, Altomare E. An update on the role of free radicals and antioxidant defense in human disease. Int J Clin Lab Res 1999; 29: 49-55.
  • [6] Christensen KI. Revision of Crataegus sect. Crataegus and Nothosect. Cratae guineae (Rosaceae-Maloideae) in the Old World. Syst. Bot. Monograms 1992; 35: 1-199.
  • [7] Ljubuncic P, Portnaya I, Cogan U, Azaizeh H, Bomzon A Antioxidant activity of Crataegus aronia aqueous extract used in traditional Arab medicine in Israel. J Ethnopharmacol 2005; 101: 153-161. [8] Baytop T. Treatment with plants in Turkey. Istanbul University Publication No. 3255, Istanbul; 1984.
  • [9] Bahorun T, Trotin F. Antioxidant activities of Crataegus monogyna extracts. Planta Med 1994; 60: 323-326.
  • [10] Bahorun T, Greiser B. Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneimittel-Forsch 1996; 46: 1086-1089.
  • [11] Rakotoarison DA, Greissier B. Antioxidant activities of phenolic extracts from flowers, in vitro callus and cell suspension cultures of Crataegus monogyna. Pharmazie 1997; 52: 60-4. [12] Horoz M, Gok E, Genctoy G, Ozcan T, Olmaz R, Akca M, Kiykim A, Gurses I. Crataegus orientalis Associated Multiorgan Hypersensitivity Reaction and Acute Renal Failure. Internal Medicine 2008; 47: 2039-2042.
  • [13] Arslan R, Bor Z, Bektas N, Meriçli AH, Oztur Y. Antithrombotic effects of ethanol extract of Crataegus orientalis in the carrageenan-induced mice tail thrombosis model. Thromb Res 2011; 127: 210-213.
  • [14] Dalar A, Konczak I. Phenolic contents, antioxidant capacities and inhibitory activities against key metabolic syndrome relevant enzymes of herbal teas from Eastern Anatolia. Ind Crop Prod 2013; 44: 383-390.
  • [15] Kim SH, Cheon HJ, Yun N, Oh ST, Shin E, Shim KS. et al. Protective effect of a mixture of Aloe vera and Silybum marianum against carbon tetrachloride-induced acute hepatotoxicity and liver fibrosis, J Pharmacol Sci 2009; 109(1): 119-27.
  • [16] Celik I, Temur A, Isık I. Hepatoprotective role and antioxidant capacity of pomegranate (Punica granatum L.) flowers infusion against trichloroacetic acid-exposed in rats. Food Chem Toxicol 2009; 47: 145-149.
  • [17] Dogan A, Celik I. Hepatoprotective and antioxidant activities of grape seeds against ethanol-induced oxidative stress in rats. Br J Nutr 2011; 107: 45-51.
  • [18] Yurt B, Celik I. Hepatoprotective effect and antioxidant role of sun, sulphited-dried apricot (Prunus armeniaca L.) and its kernel against ethanol-induced oxidative stress in rats. Food Chem Toxicol 2011; 49(2): 508-513.
  • [19] Jain SK, McVie R, Duett J, Herbst JJ. Erythrocyte membrane lipid peroxidation and glycolylated hemoglobin in diabetes. Diabetes 1989; 38: 1539-1543.
  • [20] Beutler E, Dubon O, Kelly M. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 61: 882-888.
  • [21] Mannervik B, Guthenberg C. Glutathione S-transferase (Human Plasenta). Method Enzymol 1981; 77: 231–235.
  • [22] Paglia DE, Valentine WN. Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70: 158.
  • [23] McCord JM, Fridovich I Superoxide dismutase, Anenzymatic function for erythrocuprein (hemocuprein). J Biol Chem 1969; 244: 6049-6053.
  • [24] Aebi H Catalase, In Methods of Enzymatic Analysis (Bergemeyer, H U.,ed) New York, NY, US: Academic Press: 1974. p. 673-684.
  • [25] Haagen L, Brock AA new automated method for phenotyping arylesterase (E.C. 3.1.1.2) based upon inhibition of enzymatic hydrolysis of 4-nitrophenyl acetate by phenyl acetate. Eur J Clin Chem Clin Biochem 1992; 30:391-395.
  • [26] Sallie R, Tredger JM, William R. Drugs and the liver. Part I. Testing liver function. Biopharm Drug Disp 1991; 112:251-259.
  • [27] Recknagel RO. A new direction in the study of carbon tetrachloride hepatotoxicity. Life Science 198; 33(5): 401-8.
  • [28] Turkdogan MK, Ozbek H, Yener Z, Tuncer I, Uygan I, Ceylan E. The role of Urtica dioica and Nigella sativa in the prevention of carbon tetrachloride induced hepatotoxicity in rats. Phytother Res 2003; 17: 942-946.
  • [29] Suzek H, Celik I, Dogan A, Yildirim S. (2016) Protective effect and antioxidant role of sweetgum (Liquidambar orientalis) oil against carbon tetrachloride-induced hepatotoxicity and oxidative stress in rats. Pharm Biol 54 (3): 451-457.
  • [30] Suzek H, Celik I, Dogan A. Nephroprotective Hepatoprotective Potential and Antioxidant Role of Carob Pods (Cerotonia siliqua L.) against Carbon Tetrachloride-induced Toxicity in Rats. Ind J Pharm Edu Res 2017; 51(2). 312-320.
  • [31] Chidambara Murthy KN, Rajesha J, Vanitha A, Swamy MM, Ravishankar GA. Protective effect of Dunaliella salina-a marine micro alga, against carbon tetrachloride-induced hepatotoxicity in rats. Hepatol Rese 2005; 33(4):313-9.
  • [32] Freeman BA, Crapo JD. Hyperoxia increases oxygen radical production in rat lung and lung mitochondria. J Biol Chem 1981; 256: 10986-10992.
  • [33] Smith GJ, Litwack G. Roles of ligandin and the glutathione S-transferases in binding steroid metabolites, carcinogens and other compounds. Rev Biochem Toxicol 1980; 2: 1-47.
There are 31 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Articles
Authors

İsmail Çelik 0000-0003-2199-6348

Project Number FYL-2017-5755
Publication Date December 31, 2021
Published in Issue Year 2021 Volume: 10 Issue: 2

Cite

APA Çelik, İ. (2021). Evaluation of Antioxidant and Protective Role of Hawthorn Fruit Lyophilized Extract Against Carbon Tetrachloride Toxicity in Rat. Türk Doğa Ve Fen Dergisi, 10(2), 42-47. https://doi.org/10.46810/tdfd.850150
AMA Çelik İ. Evaluation of Antioxidant and Protective Role of Hawthorn Fruit Lyophilized Extract Against Carbon Tetrachloride Toxicity in Rat. TJNS. December 2021;10(2):42-47. doi:10.46810/tdfd.850150
Chicago Çelik, İsmail. “Evaluation of Antioxidant and Protective Role of Hawthorn Fruit Lyophilized Extract Against Carbon Tetrachloride Toxicity in Rat”. Türk Doğa Ve Fen Dergisi 10, no. 2 (December 2021): 42-47. https://doi.org/10.46810/tdfd.850150.
EndNote Çelik İ (December 1, 2021) Evaluation of Antioxidant and Protective Role of Hawthorn Fruit Lyophilized Extract Against Carbon Tetrachloride Toxicity in Rat. Türk Doğa ve Fen Dergisi 10 2 42–47.
IEEE İ. Çelik, “Evaluation of Antioxidant and Protective Role of Hawthorn Fruit Lyophilized Extract Against Carbon Tetrachloride Toxicity in Rat”, TJNS, vol. 10, no. 2, pp. 42–47, 2021, doi: 10.46810/tdfd.850150.
ISNAD Çelik, İsmail. “Evaluation of Antioxidant and Protective Role of Hawthorn Fruit Lyophilized Extract Against Carbon Tetrachloride Toxicity in Rat”. Türk Doğa ve Fen Dergisi 10/2 (December 2021), 42-47. https://doi.org/10.46810/tdfd.850150.
JAMA Çelik İ. Evaluation of Antioxidant and Protective Role of Hawthorn Fruit Lyophilized Extract Against Carbon Tetrachloride Toxicity in Rat. TJNS. 2021;10:42–47.
MLA Çelik, İsmail. “Evaluation of Antioxidant and Protective Role of Hawthorn Fruit Lyophilized Extract Against Carbon Tetrachloride Toxicity in Rat”. Türk Doğa Ve Fen Dergisi, vol. 10, no. 2, 2021, pp. 42-47, doi:10.46810/tdfd.850150.
Vancouver Çelik İ. Evaluation of Antioxidant and Protective Role of Hawthorn Fruit Lyophilized Extract Against Carbon Tetrachloride Toxicity in Rat. TJNS. 2021;10(2):42-7.

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