Sıçanlarda Pentilentetrazol ile İndüklenen Akut Epilepsi Modelinde Valproik Asidin NO/cGMP Üzerindeki Etkileri

Abstract

Epilepsi, tekrarlayan nöbetlerle nöronal hasara, bilinç kaybına neden olan bir hastalıktır. Nitrik oksit (NO); beyinde nöromodülatör olarak ve hücreler arasında yayılabilen bir gazdır. Fizyolojik olaylarda, iltihaplanma ve dejeneratif hastalıklar gibi birçok hastalığın patolojisinde önemli rolü vardır. Bu araştırmanın amacı, sıçanlarda pentilentetrazol ile indüklenen deneysel akut epileptik modelde valproik asidin NO/cGMP yolağının aktivitesini araştırmaktır.Çalışmada 18 adet yetişkin erkek Wistar Albino sıçan kullanıldı. Sıçanlar rastgele kontrol grubu,pentilentetrazol (PTZ) 45 mg kg-1, valproik asit (VPA) 150 mg kg-1 olarak 3 gruba (n=6) ayrıldı. PTZ uygulamasından 24 saat sonra tüm sıçanların beyin dokuları çıkarıldı, korteks ve hipokampus ayrıldı.PTZ hipokamp ve korteks NO/cGMP düzeylerini kontrole göre artırırken (p <0,01), VPA hipokamp ve korteks NO düzeylerini PTZ'ye göre düşürdü (p<0,001). Ancak VPA korteks cGMP düzeylerini düşürürken (p <0,05), hipokampus cGMP düzeylerini değiştirmedi (p> 0,05). Bu çalışma, VPA'nın antiepileptik aktivitesini NO/cGMP yolu ile gösterebileceğini düşündürmektedir.

Keywords

• Epilepsi
• Pentilentetrazol (PTZ)
• Valproik asit (VPA)
• NO/cGMP

The Effects of Valproic Acid on NO/cGMP in Pentylenetetrazole-Induced Acut Epilepsy Model in Rats

Abstract

Epilepsy is a disease which causes neuronal damage and loss of consciousness in consequence of recurrent seizures. Nitric oxide as a neuromodulator in brain is a gas which can penetrate into cells. It has a significant role on physiological cases,pathology of many diseases such as inflammation and degenerative diseases. The purpose of this research is to search the activity of NO/cGMP pathway of valproic acid in an experimental acuteepileptic model which is induced with pentylenetetrazol in rats.18 adult male Wistar Albino rats were used in the study. The rats were randomly divided into 3 groups (n=6) as control group, pentylenetetrazol (PTZ+salin) 45 mg kg-1, valproic acid (PTZ+VPA) 150 mg kg-1. After 24 hours of PTZ application, all rats brain tissues were removed and then cortex and hippocampus were separated. While PTZ increased the hippocampus and cortex NO/cGMP levels compared to control (p <0.01), VPA decreased the hippocampusand cortex NO levels by comparison with PTZ (p <0.001). On the other hand, while VPA decreased cortex cGMP levels (p<0,05) it did not change cGMP levels in hippocampus (p> 0.05). This study has suggested that VPA can show antiepileptic activity via NO/cGMP pathway.

Keywords

• Epilepsy
• Pentylenetetrazole
• Valproin Acid
• NO/cGMP

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