Evaluation of the Efficacy of Propolis Extracts Based on Different Solvents Against Some Plant Pathogenic Fungi

Year 2024, Volume: 13 Issue: 3, 127 - 133, 26.09.2024

Abdullah Güller , Işıl Saraç Sivrikaya , Ersin Karakaya , Gözdenur Çakar

https://doi.org/10.46810/tdfd.1493162

Abstract

Propolis is a bee product produced as a natural defense mechanism by bees to protect their hives. It consists of plant resins, secretions from bees, and other substances collected from plants, containing biologically active compounds with antimicrobial, antioxidant, and anti-inflammatory properties. Due to these characteristics, propolis finds applications in various fields. In this study, pure propolis extracts obtained by DMSO, ethanol, methanol, glycerol, acetone and supercritical CO2 extraction of propolis were tested for their antifungal activity against three phytopathogenic fungi, Fusarium oxysporum, Alternaria alternata and Verticillium spp. Antifungal activity tests were conducted in vitro using zone inhibition measurements on PDA medium. Our findings revealed that the antifungal efficacy of propolis and its impact on mycelial growth parameters varied depending on the type of propolis extract used, demonstrating a dose-dependent relationship. In the present study, the ethanol + propolis preparation was found to be more successful in inhibiting the growth of fungal hyphae at a dose of 200 µl for all fungi compared to other solvents. The other solvents showed different levels of inhibition depending on the fungal species. In general, the acetone, DMSO, and glycerol preparations of propolis were less effective in the inhibition of fungal growth. The results obtained indicate that ethanol-based propolis extracts have potential for the control of agriculturally important phytopathogenic fungi.

Keywords

Propolis, Fusarium oxysporum, Alternaria alternata, Verticillium dahliae, antifungal effect

Project Number

Çalışmanın projesi bulunmamaktadır

References

• Banskota AH, Nagaoka T, Sumioka LY, Tezuka Y, Awale S, Midorikawa K, et al. Antiproliferative activity of the Netherlands propolis and its active principles in cancer cell lines. In Journal of Ethnopharmacology. 2002;80(1):67–73.
• Abd-El-Rhman AM. Antagonism of Aeromonas hydrophila by propolis and its effect on the performance of Nile tilapia, Oreochromis niloticus. In Fish & Shellfish Immunology. 2009;27(3):454–459.
• Bankova V, Galabo, AS, Antonova D, Vilhelmova N, Di Perri B. Chemical composition of Propolis Extract ACF® and activity against herpes simplex virus. Phytomedicine. 2014; 21(11):1432-1438.
• Ghisalberti EL. Propolis:a review.Bee World. 1979;60:59-84.
• Castaldo S, Capasso F. Propolis, an old remedy used in modern medicine. Fitoterapia. 2002;73(1):1-6.
• Toreti VC, Sato HH, Pastore GM, Park YK. Recent progress of propolis for its biological and chemical compositions and its botanical origin. Evidence Based Complementary Alternative Medicine. 2013;697390. DOI: 10.1155/2013/697390.
• Hegazi AG. Propolis an overview. Congreso Internacional de Propóleos ,Buenos Aires 1-2nd, September 2000.
• Basim E, Basim H, Özcan M. Antibacterial activities of Turkish pollen and propolis extracts against plant bacterial pathogens. Journal of Food Engineering. 2006;77(4):992-996. DOI: 10.1016/j. jfoodeng.2005.08.027
• Marcucci MC. Propolis: Chemical Composition, Biological Properties And Therapeutic Activity. In Apidologie. 1995;26:83-99.
• Güney F, Yılmaz M. Propolisin kimyasal içeriği ile antibakteriyel, antiviral, antitümör, antifungal ve antioksidan aktivitesi. Arıcılık Araştırma Dergisi. 2013;10: 25-28.
• Lisa M, Leifertova I, Baloun J. Fungistatic effect of propolis. Folia Pharm Univ Carol. 1989;13:29-44.
• Matsuno T, Chen C, Basnet P. A tumoricidal and antioxidant compound isolated from an aqueous extract of propolis. Medical Science Research. 1997;25:583–584.
• Bogdanov S. Propolis: Composition, Health, Medicine: A Review. Bee Product Science, 2012. www.bee-hexagon.net
• Yang SZ, Peng LT, Su XJ, Chen F, Cheng YJ, Fan G, et al. Bioassay-guided isolation and identification of antifungal components from propolis against Penicillium italicum. Food Chemistry. 2011;127(1):210-215. http://dx.doi.org/10.1016/j.foodchem.2010.12.011
• Mattiuz BH, Ducamp-Collin MN, Mattiuz CFM, Vigneault C, Marques KM, Sagoua W, et al. Effect of propolis on postharvest control of anthracnose and quality parameters of ‘Kent’ mango. Scientia Horticulturae. 2015;184:60-168. DOI: 10.1016/j.scienta.2014.12.035
• Meneses EA, Durango DL, García CM. Antifungal activity against postharvest fungi by extracts from Colombian propolis. Química nova. 2009;32:2011-2017.
• Curifuta M, Vidal J, Salazar LA, Sánchez-Venegas J, Alvear M, Contreras A. The in vitro antifungal evaluation of a commercial extract of Chilean propolis against six fungi of agricultural importance. Ciencia e Investigación Agraria. 2012;39(2):347-359.
• Ali A, Wei YZ, Mustafa MA. Exploiting Propolis as an Antimicrobial Edible Coating to Control Post-harvest Anthracnose of Bell Pepper. Packaging Technology and Science. 2014. DOI: 10.1002/pts.2088
• Dudoit A, Mertz C, Chillet M, Cardinault N, Brat P. Antifungal activity of Brazilian red propolis extract and isolation of bioactive fractions by thin-layer chromatography-bioautography. Food Chemistry. 2020;327:1-8. DOI: 10.1016/j.foodchem.2020.127060
• Agrios GN. Plant pathology. 5th Edition, Elsevier Academic Press, Amsterdam, 2005;26-27:398-401.
• Lucas JA, Hawkins NJ, Fraaije BA. The evolution of fungicide resistance. Advances in applied microbiology. 2015;90:29-92.
• Quiroga EN, Sampietro DA, Soberon JR, Sgariglia MA, Vattuone MA. Propolis from the northwest of Argentina as a source of antifungal principles. Journal of Applied Microbiology. 2006;101(1):103-110. DOI: 10.1111/j.1365-2672.2006.02904.x
• Agüero MB, Svetaz L, Baroni V, Lima B, Luna L, Zacchino S, Tapia A. Urban propolis from San Juan province (Argentina): Ethnopharmacological uses and antifungal activity against Candida and dermatophytes. Industrial Crops and Products. 2014;57:166-173. DOI: 10.1016/j.indcrop.2014.03.009
• Bosio K, Avanzini C, D’Avolio A, Ozino O, Savoia D. In vitro activity of propolis against Streptococcus pyogenes. Letters on Applied Microbiology. 2000;31:174-177. DOI: 10.1046/j.1365- 2672.2000.00785.x
• Ronald MA. Microbiologia, Compania Editorial Continental S. A. de C. V., Mexico, 1 D. 1990. F. pp. 505.
• Deans SG, Sobada KP. Antimicrobial Properties of Marjoram (Origanum marjorana L.) Volatile Oil. Flavour Fragr. J. 1990;5:187-190.
• Arslan S, Perçin D, Silici S. The in vitro effects of propolis extracts prepared with different solvents on mutans streptococci. J Health Sci. 2010;19:68-73.
• Davari M, Ezazi R. Mycelial inhibitory effects of antagonistic fungi, plant essential oils and propolis against five phytopathogenic Fusariumspecies. Archives of Microbiology. 2022; 204:480.
• Er Y. In vitro and in vivo antimicrobial activity of propolis extracts against various plant pathogens. Journal of Plant Diseases and Protection. 2021;128(3):693-701.
• Costantin CR, de Oliveira HG, Roese LSM, Pancera MR, Silvestre WP, Siqueira GR, et al. Evaluation of the antifungal activity of propolis extracts from stingless bees on phytopathogenic fungi. Research, Society and Development. 2022;11(16):e445111638445-e445111638445.
• Moura SALD, Negri G, Salatino A, Lima LDDC, Dourado LPA, Mendes JB, et al. Aqueous extract of Brazilian green propolis: primary components, evaluation of inflammation and wound healing by using subcutaneous implanted sponges. Evidence-Based Complementary and Alternative Medicine. 2011.
• Özcan M, Ünver A, Ceylan DA,Yetisir R. Inhibitory effect of pollen and propolis extracts. Food/Nahrung. 2004;48(3):188-194.
• Ertürk Ö, Yavuz C, Sıralı C. The Antimicrobial Activity of Propolis from Ordu Province of Turkey. HARUM. 2011;28:11-16.
• Ghasemi FS, Eshraghi SS, Andalibi F, Hooshyar H, Kalantar- Neyestanaki D, Samadi A, et al. Anti-Bacterial Effect of Propolis Extract in Oil Against Different Bacteria. Zahedan J. Res. Med. Sci. 2017;19(3):7225.
• Ugur A, Arslan T. An in vitro study on antimicrobial activity of propolis from Mugla province of Turkey. Journal of Medicinal Food. 2004;7(1):90-94.