Ultrastructural examination of the effects of malathion on kidney: An experimental study

Abstract

Introduction and purpose: Malathion is an insectisid widely used in agriculture in our daily life for killing insects. On behalf of its benefits, it has harmful and toxic effects on ecosystems, and on human. In our study we aimed to evaluate whether the disorders in kidney caused by malathion intoxication are related to the cellular damages light microscopically and electron micro-scopically. Materials and methods: 28 Balb-c mice were used. The mice were grouped as; control (n: 7), LD50 (n: 7), LD50/2 (n: 7), LD50/4 (n:7) respectively. The mice were given malathion orally at LD50, LD50/2, LD50/4 doses. 24 hours later, kidneys were harvested and after routine histological procedures kidney tissue cross sections were evaluated by light microscope and electron microscope. Results: Especially perivascular mononuclear cell infiltrations, erythrocyte clusters in tubule lumens,thyroidisations of tubules and interstitial erythrocyte extravasations were seen at the histological sections of the kidneys taken from the malathion given groups. Electron microscopically, irregularity of the glomeruler basement membrane and mesengial hypertrophy were found. When the results of the control group and the malathion given groups compared, it was found statistically significant (p<0.05). Conclusion: We think that the systemic symptoms seen in organophosphate intoxications may be related to the cellular damage in involved organs

Keywords

• Malathion, kidney, ultrastructure experimental study

Malathion’un böbrek üzerine etkilerinin ultrastrüktürel incelenmesi: Deneysel çalışma

Öz

Giriş ve amaç: Malathion; tarımda ve günlük yaşamda böcekleri öldürmek amacıyla yaygın olarak kullanılan bir insektisittir. Yararları yanında çeşitli nedenlerle insanlara ve tüm ekosisteme zararlı olabilen bu ürünün istenmeyen en önemli etkisi zehirlenmelerdir. Çalışmamızda Malathion ile zehirlenmede görülen böbrek dokusundaki bozuklukların hücresel hasara bağlı olup olmadığını ışık mikroskobik ve elektron mikroskobik olarak incelemeyi amaçladık. Gereç ve yöntem: Çalışmamızda 28 adet Balb-c türü fare kullanıldı. Bir grup (n: 7) kontrol grubu olurken diğer 3 gruba LD50 (n: 7), LD50/2 (n: 7), LD50/4 (n: 7) dozunda malathion verildi. Deneyden 24 saat sonra böbrek dokuları alındı. Rutin histolojik takip işlemlerinden sonra elde edilen dokulara ışık mikroskobik ve elektron mikroskobik değerlendirme yapıldı. Bulgular: Malathion verdiğimiz gruplardan elde edilen böbrek dokusu incelendiğinde, özellikle damar çevresinde belirgin mononükleer hücre infiltrasyonu tubulus lümenlerinde eritrosit kümeleri, tubuluslarda tiroidizasyon, intertisyumda eritrosit ekstravazasyonu gözlendi. Elektronmikroskobik incelenmesinde özellikle glomerüler bazal membran kalınlığının düzensizleştiği ve mezangial hipertofi saptandı. Malathion uygulanan gruplara ait bulgular ile kontrol grubu karşılaştırıldığında, sonuçlar istatistiksel olarak anlamlı bulundu (P<0.05). Sonuç: Organofosfat bileşikleri ile oluşan zehirlenmelerde görülen sistemik yakınmaların nedeninin ilgili organlarda oluşan hücresel hasar olabileceğini düşünmekteyiz

Anahtar Kelimeler

• Malathion, böbrek ultrastürüktürü, deneysel çalışma

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