Effect of Wheat Germ Oil on Wound Healing: An In Vitro Study in Fibroblast Cells

Abstract

Purpose: Wound healing is a set of mechanisms that are activated to restore structurally damaged tissue. There are many studies aimed at accelerating wound healing. In this context, products obtained from plants come to the fore. In this article, the effects of wheat germ oil (WGO), which is known to have antioxidant and anti-inflammatory properties, on wound healing were investigated by in vitro method. Material and Methods: Wheat germ oil and α-tocopherol were applied to L929, a healthy fibroblast cell line, at different doses for 24, 48 and 72 hours. Cell viability was measured by XTT colorimetric method. In vitro wound healing model was applied at the dose where the oil was effective. Obtained results were analyzed statistically. Results: As a result of the application of α-tocopherol to L929 cells at different doses, it was observed that there was no significant contribution to cell proliferation compared to the control group. However, WGO was observed to significantly increase proliferation at the 100 ng/ml concentration. In the wound healing model, cells treated with WGO at 48 hours were observed to proliferate faster and invade the wound site more rapidly. (p<0.05) Conclusion: The data obtained in our study showed that WGO contributed positively to wound healing. It was determined that this effect was not related to the α-tocopherol in the content of WGO. Although the effect of WGO on wound healing was seen to be positive in our study, our study should be supported by in vivo and clinical studies.

Keywords

• Cell viability
• Wheat germ oil
• Wound healing
• α-tokoferole.

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