The Anticancer Effect of Cannabinoid 2 Agonist L-759,633 on C6 and SH-SY5Y Cell Lines

Abstract

Purpose: Previous studies have shown that cannabinoid 2 agonists have anticancer effects on different cancer cell lines. However, the effect of L-759,633 on the C6 and SH-SY5Y cell lines is still unclear. The current study was designed to investigate the potential cytotoxic effects of a cannabinoid 2 agonist L-759,633 on the C6 and SH-SY5Y cell lines. Material and Methods: In this study, the C6 and SH-SY5Y cell lines were used. For each cell line two cell groups were prepared to examine the effect of L-759,633 on C6 glioma and SH-SY5Y cell death. It was not applied any treatment to the cells in the control groups. Various concentrations (5, 10, 20, 40 and 80 μM) of L-759,633 was applied to the cells in the L-759,633 groups. After 24 hours the cell viability was examined using XTT assay. Total oxidant status (TOS) and Total antioxidant status (TAS) in the cells were measured by commercial kits. Results: L-759,633 at the concentrations of 5, 10, 20, 40 and 80 μM significantly decreased the cell viability in C6 cells (p < 0.001). It also significantly increased the TOS levels (p < 0.001) whereas didn’t alter the TAS levels (p > 0.05). On the other hand this agent did not reduce SH-SY5Y cell viability. Conclusion: L-759,633 has antiproliferative effect on C6 cells. Additionally, one of the potential mechanisms involved in this effect is activation the oxidant generation. However this drug didn’t show antiproliferative effect on SH-SY5Y cell. Keywords: L-759,633, Oxidative Stress, cell viability, C6 Glioma, SH-SY5Y cell.

Keywords

• L-759,633
• Oxidative Stress
• C6 Glioma
• SH-SY5Y cell

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