Ağız Çalkalama Sularının Gargara In Vitro Antifungal Aktiviteleri

Öz

Etken maddeleri, klorheksidin diglukonat, sanguinarin ekstrakt, setilpiridinium klorid, heksetidin, triklosan vetimol olan altı ticari ağız çalkalama suyunun in vitroantifungal aktiviteleri araştırıldı. Test organizması olarak çeşitliklinik örneklerden izole edilmiş Candida albicans,Saccharomyces cerevisiae, Candida parapsilosis, Candidaguilliermondii, Candida krusei, Candida tropicalis, Candida kefyr veCandida glabrata kullanıldı. Bu gargaralarınin vitroantifungal etkileri; MİK, MFK ve “kill-time” ları belirlenerek değerlendirildi. Tüm gargaraların MİK ve MFKdeğerleri arasında en fazla iki katlık bir fark olduğu, özellikle klorheksidin diglukonat'ın test edilen maya türlerine karşıdiğer gargaralara oranla çok daha düşük konsatrasyonlarda inhibitör ve fungisidal etki gösterdiği saptandı. Tüm testorganizmaları için en kısa “kill-time” a ≤15 saniye klorheksidin diglukonat ve setilpiridinium klorid’in, en uzun >180 saniye ise sanguinarin ekstrakt’ın sahip olduğu saptandı. Sonuç olarak klorheksidin diglukonat ve setilpiridinium klorid içeren gargaraların mayalara karşı in vitroolarakdaha etkili olduğu ancak in vivoetkilerinin klinik denemelerle desteklenmesi gerektiği düşünülmektedir

Anahtar Kelimeler

• Ağız çalkalama suları, antifungal aktivite, kill-time

The In Vitro Antifungal Activities Of Different Mouthrinses

Abstract

In this study, we aimed to investigate the in vitro antifungal activities of six different commercial mouthrinses;chlorhexidine digluconate, sanguinarine extract, cetylpyridinium chloride, hexetidine, triclosan and thymol. Wetested these agents activities against clinical isolates of Candida albicans, Saccharomyces cerevisiae, Candidaparapsilosis, Candida guilliermondii, Candida krusei, Candida tropicalis, Candida kefyrand Candida glabrata. Thein vitro activities of these agents were evaluated by determining the MIC and MFC of each product. The kill time ofeach drug was also determined. The difference between MIC and MFC values of six agents was not higher than twofold. It was found that chlorhexidine digluconate had inhibitory and fungicidal properties at lowest concentration whencompared to other products. While the kill-time of chlorhexidine digluconate and cetylpyridinium chloride was equalor less than 15 seconds, period > 180 seconds was required for sanguinarine extract.In conclusion, although all antimicrobial mouthrinses included in this study possess in vitroantifungalproperties, chlorhexidine digluconate and cetylpyridinium chloride are more active than other products tested. Thein vivoactivities of these products need to be supported by further clinical trials

Keywords

• Mouthrinses, antifungal activity, kill-time

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