Daminozit'in Tavşan Karaciğer Glutatyon-S-Transferaz Aktivitesine Etkisinin Araştırılması

Öz

Çalışmada tavşan karaciğerinden izole edilen Glutatyon-S-Transferaz'ın etkili bir pestisit olan daminozit ileetkileşimi incelenmiştir. Glutatyon -S-Transferaz enzimi in vitro şartlarda değişik dozlarda daminozit ile muameleedilerek, reaksiyonları gözlenmiştir. Bulgular 1-kloro 2,4 dinitro benzen CDNB substrat olarak kullanıldığında eldeedilen aktivite ile karşılaştırılmıştır. Regresyon ve korelasyon analizi yapılmış ve sonuçlar spesifik aktivite olarakverilmiştir

Anahtar Kelimeler

• Daminozit, Glutatyon -S-transferaz

An Investigation On Effect Of Daminozide To The Glutation-S-Transferase Activity In Rabbit Liver

Abstract

We analyzed the interaction between glutation-S-transferase, which is isolated from the liver of rabbits, anddaminozide which is an effective pesticide. Glutation-S-transferase enzymes were treated with daminozide in vitroconditions at various doses, and the reactions were recorded. The observed activities were compared with theactivity of 1-chloro 2, 4 dinitrobenzen substrate and a regression analaysis was performed to investigate thecorrelation. The result were expressed as spesific activites

Keywords

• Daminoside, Glutation S-Transferase

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