PATOJENİK DERMAFİTLERİN MONOFLOROMETİLAGMATİNE DUYARLILIK MEKANİZMASININ ARAŞTIRILMASI

Öz

Poliaminler, bitki, hayvan, bakterileri ve mantarların normal büyüme ve gelişmesi için gerekli olan temel alifatikkatyonlardır. Microsporumve Trichophytonsırasıyla ornitin ODC ve arginin-dekarboksilazın ADC suicideinhibitörleri olan diflorometilarjinin DFMA ve diflorometilornitin DFMO büyüme inhibisyonuna in-vitroduyarlılık göstermişlerdir. Bununla birlikte DFMA’ya olan duyarlılığı destekleyecek bilgiler net değildir. Bu konudayapılan başlangıç çalışmalarda, her iki mantarın ekstrelerinde gözlenebilir. ADC, arginaz veya agmatin ürehidrolaz AUH ’a olan aktiviteyi saptayamamışlardır. Bizim çalışmamızda arginaz, AUH ve ADC aktivitelerinin tekrarincelenmesinin yanı sıra dermatofitlerin AUH’ı yok edici bir inhibitör olan Monoflorometilagmatine tithylagmatine olanduyarlılıkları incelenmiştir. Elde ettiğimiz bulgular dermatofitlerde AUH’ın olduğu gibi arginazın da mevcudiyetinigöstermiş ve ayrıca Microsporum,Trichophyton’da DFMA ve DFMAg açısından her iki mantarın arginaz veAUH enzimlerinin Kmsve Kıs Kinetiği=Lineweawer-Burke değerleri bulunmuştur

Anahtar Kelimeler

• Dekarboksilaz, monoflorometilagmatin, mikrosporum, poliamamin sentezi

The Nature Of The Susceptibility Of Pathogenic Dermatophytes To Monofluoromethylagmatıne MFMag

Abstract

Polyamines are essential aliphatic cations required for normal growth and develepment in plants, animals,bacteria and fungi. M i c r o s p o r u m, and T r i c h o p h y t o nwere in-vitro susceptible to growth inhibition bydifluoromethylornithine DFMO and difluoromethylarginine DFMA , suicide inhibitors of the ornithine ODC andarginine decarboxylase ADC respectively. However, the basis for the susceptibility to DFMA was unclear as initialstudies failed to find detectable ADC, arginase nor agmatine ureohydrolase AUH activities in extracts fromeither genus. We have re-examined the arginase, AUH and ADC activities as well as dermatophytes’susceptibilities to MFMAg, a suicide inhibitor of AUH. The data demonstrated existence of arginase as well asAUH’ in these dermatophytes and found the Lineweayer-Burke Kmsand K1sof arginase and AUH withrespect to DFMA and MFMAg in both Microsporum, and Trichophyton

Keywords

• Decarboxylase, monofluoromethylagmatine, Microsporum, polyamines

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