Çeşitli Dondurma Yöntemlerinin Hep-2 Ve Tavşan Böbrek Hücrelerinin Kriyoprezervasyonunda Hücre Canlılığına Etkisi

Öz

Bu çalışmada bovin serum albumininin, primer tavşan böbrek hücre kültürü ile HEp-2 devamlı hücre kültürlerininkriyoprezervasyonunda hücre canlılığı üzerindeki rolü araştırıldı. Öncelikle hücrelerin yavaş, orta hızlı ve hızlıdondurma metodlarıyla kriyoprezervasyonu yapıldı. Kriyoprotektan madde olarak dimetil sülfoksit, gliserol ve Rowekarışımı kullanıldı. Hücreler %5 ve %10’luk kriyoprotektan madde ve 0, 5, 10, 20 ve 30g/mL bovin serum albuminiçeren dondurma solüsyonları ile donduruldu. Deneylerde en yüksek hücre canlılığı yavaş dondurma metodundaelde edilirken, bu metotda dondurma solüsyonuna katılan bovin serum albuminin konsantrasyonuyla doğru orantılıolarak hem primer tavşan böbrek hem de HEp-2 devamlı hücrelerinin canlılığı üzerinde istatiksel olarak anlamlı birartış meydana getirdiği tespit edildi p0.05

Anahtar Kelimeler

• Kriyoprezervasyon, hücre kültürü, bovin serum albumin, kriyoprotektan ajanlar

The Effect Of The Various Freezing Methods On The Cell Viability Of Cryopreserved Hep-2 And Rabbit Kidney Cells

Abstract

In this study, the role of bovine serum albumin on the viability of cryo-preserved primary rabbit kidney cell- andHEp-2 continuous cell cultures was studied. Firstly, the cryopreservations of cells were done via slow, intermediateand rapid freezing methods. Dimethyl sulfoxide, glycerol and Rowe mix were used as cryoprotectant agents. Thecells were frozen with the solutions containing 5% and 10% cryoprotectant agents and 0, 5, 10, 20, 30g/mL bovineserum albumin, separately. While the highest cell viability was obtained with slow freezing method, in this method, ithas been determined that the concentration of bovine serum albumin in the freezing solution proportionally increasedthe cell viability both on primary rabbit kidney cells and HEp-2 continuous cells p0.05

Keywords

• Cryopreservation, cell culture, bovine serum albumin, cryoprotectant agents

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