18-60 Yaş Arası Türklerde Elecsys 2010 İnsülin Testinin Referans Aralığının Belirlenmesi

Abstract

Amaç: Referans aralıklar, laboratuvar test sonuçlarının değerlendirilmesinde vazgeçilmez bir öneme sahiptirler ve klinisyenlerin sağlıklı ve hastalıklı bireyler arasında bir ayırım yapmasında yardımcı olurlar. Her laboratuvarın kendi referans aralıklarını belirlemesi önerilmektedir. Bu çalışmadaki amacımız, laboratuvarımızın dokuz aylık çalışmalarından elde edilen verilerden, erkek ve kadın bireylerde Elecsys 2010 insülin testinin referans aralıklarını belirlemektir. Yöntem: Dokuz aylık sürede laboratuvarımıza başvuran hastaların insülin sonuçları analiz edildi. Erişkin bireylerin açlık insülin sonuçlarından referans aralıklar belirlendi. Uç değerler atıldıktan sonra 1280 kadın ve 300 erkek bireyin sonuçları incelendi. Çalışma yöntemi elektrokemiluminesan immunoassay ECLIA idi.Bulgular: %95 güven aralığı ile hesaplanan referans aralıkları üreticinin referans aralığından daha düşüktü. Hesaplanan referans aralıklar üretici firmanın önerdiği referans aralıklardan belirgin olarak farklı bulundu. Cinsiyetler arasında anlamlı fark saptanmadı. İnsülin test sonuçları için elde edilen referans değerleri %2,5-97,5’luk aralık için 1,7-19,8 µU/mL olarak bulundu. Sonuç: Referans aralıkları belirleyen çalışmaların, klinisyenlerin hizmet verdikleri kendi hasta popülasyonlarını tanımalarında ve değerlendirmelerinde yardımcı olacağını düşünmekteyiz

Keywords

• Referans aralık, insülin, Türkiye

Determination of reference intervals for elecsys 2010 insulin assay in 18-60 years old Turkish individuals

Abstract

Objective: Reference intervals are indispensable for interpretation of laboratory data and it helps clinicians to differentiate healthy person from sick ones. It has been suggested that each laboratory determine its own reference intervals. The aim of this study was to estimate the reference intervals for Elecsys 2010 insulin assay in male and female individuals from the data obtained from the analyses of the laboratory in a nine months period.Method: The insulin test results of the patients who applied to our laboratory in nine months were analysed. Reference intervals were determined from the results of the fasting insulin levels of the adult individuals. Insulin results of 1280 female and 300 male individuals were evaluated after exclusion of the outliers. The assay method was electrochemiluminescence immunoassay ECLIA . Results: Calculation of reference intervals with 95 % confidence limits showed lower values than the defined reference values by manufacturer. For insulin, the determined reference interval for our data was significantly different from the provided by manufacturer. There was no significant difference between the genders. Calculated reference intervals for the insulin assay were found as 1,7-19,8 µU/mL for 2,5th-97,5th percentile range.Conclusion: We indicate that the studies confirming the reference intervals will be helpful to clinicians to determine and evaluate their patient populations that they served

Keywords

• Reference values, insulin, Turkey

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