Francisella tularensis antikorları ile Brucella çapraz reaksiyonlarının araştırılması

Öz

Amaç: Francisella tularensis antikorları ile Brucella, Legionella, Yersinia ve Mycoplasma türleri arasında çapraz reaksiyonlar bildirilmiştir. F.tularensis liposakkarit yapısında bulunan Brucella O-polisakkaritindeki benzer antijenik yapılar serolojik testlerde çapraz reaksiyonlara neden olabilmektedir. Aglütinasyon testlerinde F.tularensis ve Brucella abortus arasındaki çapraz reaksiyonlar sonuçların yorumlanmasını güçleştirebilir. Yöntem: Brucella ve F.tularensis arasındaki çapraz reaksiyonların sıklığı ve bu reaksiyonlardan sorumlu olan antikorlar mikroaglütinasyon, Brucella ELISA ve 2-merkaptoetanol mikroaglütinasyon testleri ile araştırılmıştır. Bulgular: 260 tularemi pozitif örneğin 49 %18.8 ’unda B.abortus antijeniyle çapraz reaksiyonlar saptanırken, bruselloz pozitif 252 örneğin 23 %9.1 ’ünde F.tularensis antijeni ile çapraz reaksiyon gözlenmiştir. Otuzaltı tularemi %73.5 pozitif serumda Brucella çapraz reaksiyon titreleri 1:20-1:80 iken, 13 örnekte %26.5 ise ≥1:160 titrelerde çapraz reaksiyon gözlenmiştir. 2-ME testi ile

Anahtar Kelimeler

• Tularemi, Brucella, Çapraz reaksiyon, Aglütinasyon, 2-Merkaptoetanol

Investigation of cross-reactions with Francisella tularensis antibodies to Brucella

Abstract

Objective: Cross-reactions with Francisella tularensis antibodies to Brucella, Legionella, Yersinia, and Mycoplasma species have been described. F.tularensis LPS is able to induce serological crossreactions indistinguishable from brucellosis due to a similar immunodominant epitope in the Brucella O-polysaccharide. In agglutination tests, cross-reaction occurred only between F.tularensis and Brucella abortus, cross reactivity with Brucella can confuse interpretation of the results. Method: The aim of this study was to assess cross reactions and whether IgM or IgG antibodies are responsible for Brucella and F.tularensis crossreactions, by the use of microagglutination, Brucella ELISA and 2-mercaptoethanol microagglutination test. Results: Cross-reaction agglutinin titers to B.abortus antigen were found in 49 of 260 18.8% tularemia positive serum specimens, and cross-reaction titers to F.tularensis antigen were found in 23 of 252 9.1% Brucella positive serum specimens. While thirty six 73.5% tularemia positive serum titers had Brucella cross reactivity at the titers of 1:20-1:80, cross-reaction with ≥1:160 titers was found in 13 samples 26.5% . The cross-reaction titers to B.abortus antigen were reduced to equal or less 1:40 titers by 2-ME. Cross- reactivity at the titers of 1:20-1:80 was determined in 22 of 23 Brucella positive samples

Keywords

• Tularemia, Brucella, Cross reaction, Agglutination, 2-Mercaptoethanol

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