Türkiyede yetişen Equisetum arvense, Plantago lanceolota ve Olea europaea yaprağından elde edilen ticari ekstraktların in-vitro antibakteriyel aktivitelerinin araştırılması

Öz

Amaç: Bitkiler yüzyıllardır enfeksiyon hastalıklarının tedavisi dahil olmak üzere birçok hastalığın tedavisinde kullanılmaktadır. Son yıllarda patojen mikroorganizmalara karşı kullanılan antibakteriyel maddelere direncin artmasıyla yeni bitki kaynaklı antibakteriyel madde arayışı artmıştır. Bu çalışmada, Türkiye’de yetişen Equisetum arvense, Plantago lanceolota ve Olea europaea yaprağından elde edilen ticari bitki ekstraktlarının, nozokomiyal enfeksiyonların etiyolojisinde de yer alan bazı Gram pozitif ve Gram negatif bakterilere karşı in-vitro antibakteriyel aktivitelerinin ve minimum inhibitör konsantrasyon MİK değerlerinin araştırılması amaçlandı.Yöntem: Etanol ekstraksiyonu ile elde edilmiş ticari bitki ekstraktlarının, Genişlemiş Spektrumlu Beta Laktamaz GSBL üreten Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis ve Metisilin Dirençli Staphylococcus aureus MRSA türlerine karşı antibakteriyel aktivitesi disk diffüzyon ve MİK değeri sıvı mikrodilüsyon yöntemi ile araştırıldı.Bulgular: Disk difüzyon sonuçlarına göre, tüm bitki ekstraktlarının 6.24 mg/disk yalnızca MRSA’ya karşı extracts had different MIC levels on all bacteria by broth microdilution method. And O. europaea extract showed the highest antibacterial activity in the two methods.Conclusion: According to the results of this study, particularly O. europaea leaf extract, besides other tested plant extracts are thought to be effective against to MRSA, beta lactamase procuding E. coli, K. pneumoniae, P. aeruginosa, E. faecalis. And this extracts may used in many areas like medicine and food industries. However iti is concluded that there is need for further in-vivo studies.Key Words: Anti-bacterial activity, broth microdilution, disc diffusion, nosocomialfarklı düzeylerde zon çapı oluşturduğu belirlendi. Sıvı mikrodilüsyon yöntemiyle ise tüm bitki ekstraktlarının bakteriler üzerinde farklı düzeyde MİK değerlerine sahip olduğu belirlendi. Her iki yöntemde de uygulanan eşit konsantrasyon değerlerinde sonuçlar uyumlu çıkarken, bitki türleri içerisinde en yüksek antibakteriyel aktivite O. europaea yaprak ekstraktında belirlendi ve en duyarlı bakterinin MRSA olduğu tespit edildi.Sonuç: Çalışmada elde edilen sonuçlara göre başta O. europaea yaprağı ekstraktı olmak üzere, araştırılan bitki ekstraktlarının sağlık, farmasötik, kozmetik ve gıda endüstrisi gibi birçok alanda başta MRSA olmak üzere, genişlemiş spektrumlu beta laktamaz üreten E. coli, K. pneumoniae, P. aeruginosa, E. faecalis türlerine karşı etkili olabileceği ve bu konuda yeni in vivo çalışmalara ihtiyaç olduğu sonucuna varılmıştır

Anahtar Kelimeler

• Antibakteriyel aktivite, sıvı mikrodilüsyon, disk difüzyon, nozokomiyal

Investigation of in-vitro antibacterial activities of commercial extracts prapered from Equisetum arvense, Plantago lanceolota and Olea europaea leaf grown in Turkey

Abstract

Objective: Because of increased resistance to antibacterial agents used against pathogenic microorganisms, researches about new plant derived antibacterial agents were increase in recent years. In this study it was aimed to investigate the invitro antibacterial activities and minimal inhibitory concentrations of some commercial plant extracts Equisetum arvense, Plantago lanceolota and Olea europaea leaf on some Gram positive and Gram negative bacteria which are the cause of nosocomial infections.Methods: Antibacterial activity and minimum inhibitory concentration MIC value of commercial plant extracts obtained by ethanol extractions against Extended Spectrum Beta Lactamase ESBL proceducing Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis, Methicillin Resistant Staphylococcus aureus MRSA were determined by disk diffusion and broth microdilution methods.Results: According to the disc-diffusion method results it was determined that all the plant extracts 6.24 µg/disc had antibacterial activity only on MRSA in different levels. But it was found that all the plant

Keywords

• Anti-bacterial activity, broth microdilution, disc diffusion, nosocomial

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