Kafeinin embriyonel böbrek gelişimine olan etkisine karşı melatoninin koruyucu rolü

Öz

Amaç: Oksidatif stres bozukluklarının en büyük sebeplerinden biridir. Ayrıca, melatonin vücutta oksidatif stresi azaltması nedeniyle kafeinin fetal gelişime olan olumsuz etkilerine karşı potansiyel bir tedavi yöntemi olarak görülmektedir. Bu çalışmada; kafeinin fetal böbrek gelişimine olan teratojenik etkisi ve melatonin maddesinin bu teratojeniteye karşı koruyuculuğunu biyokimyasal parametreler üzerinden incelemeyi hedeflenmiştir. TAS değeri kontrol grubunda 0,49±0,02, yüksek doz kafein grubunda 0,14±0.00 olduğu görülmüştür. Ayrıca total oksidan kapasite TOS , oksidatif stres indeksi OSI , glutatyon GSH , glutatyon disülfit GSSG , süperoksit dismutaz SOD , tiyobarbitürik asit reaktif maddeler TBARS , Kalsiyum Ca , D vitamini ve GSH/GSSG seviyeleri incelenmiştir p

Anahtar Kelimeler

• Kafein, sıçan, melatonin, böbrek

The protective role of melatonin against the effect of caffeine on embryonic kidney

Abstract

Objective: Oxidative stress is one of the major causes of embryonal developmental disorders. In addition, melatonin reduces oxidative stress in the body and is known as a potential treatment for adverse effects of caffeine on fetal development. In this study, we aimed to investigate the teratogenic effect of caffeine on fetal kidney development and the protection of melatonin against the teratogenicity in terms of biochemical parameters.Methods: 5-7 months old Wistar-Albino rats n=24 weighing 180-220 g were divided into seven groups for control, caffeine, melatonin, and caffeine+melatonin co-injection . Control group: Serum physiological SF as 1 mL/kg intraperitoneal i.p. , Sham group: 0.1 ml hanks as i.p., Low dose caffeine group: 30 mg/kg caffeine as gavage, Low dose caffeine+melatonin group: 30 mg/kg dose gavage with 10 mg/kg melatonin as i.p., High dose caffeine group: 60 mg/kg caffeine as gavage, High dose caffeine+melatonin group: 60 mg/kg dose gavage with 10 mg/kg melatonin as i.p., and Melatonin group: 10 mg/kg melatonin as i.p.Results: Total antioxidant status TAS values were 0.49±0.02 in the control group and 0.14±0.00 in the high TAS değeri kontrol grubunda 0,49±0,02, yüksek doz kafein grubunda 0,14±0.00 olduğu görülmüştür. Ayrıca total oksidan kapasite TOS , oksidatif stres indeksi OSI , glutatyon GSH , glutatyon disülfit GSSG , süperoksit dismutaz SOD , tiyobarbitürik asit reaktif maddeler TBARS , Kalsiyum Ca , D vitamini ve GSH/GSSG seviyeleri incelenmiştir p

Keywords

• Caffeine, rat, melatonin, kidney

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