Gıda mikrobiyolojisinde Enterobacteriaceae üyeleri için kromojenik ve florojenik besiyerleri

Year 2011, Volume: 68 Issue: 1, 49 - 58, 01.03.2011

Emrah Torlak

Abstract

Gıda endüstrisinde kalite güvencenin sağlanmasında ve halk sağlığının korunmasında mikrobiyolojik risklerin hızlı tespiti önemlidir. Bu nedenle gıda mikrobiyolojisinde analiz sürelerini kısaltmak amacıyla birçok alternatif metot geliştirilmiştir. Bu metotların birçoğu mikroorganizmaların spesifik enzim aktivitelerini tespit etmeye yönelik kromojenik ve florojenik substratların kullanımı esasına dayalıdır. Gıda mikrobiyolojisi laboratuvarlarında iş gücü ve zamandan tasarruf sağladıklarından dolayı kromojenik ve florojenik substratlar içeren besiyerlerinin kullanımı artarak devam etmektedir. Bu derlemede, gıda mikrobiyolojisi bakımından en önemli familya olan Enterobacteriaceae familyası üyesi bakterilerin tespit edilmesi ve sayılmasına yönelik kromojenik ve florojenik besiyerleri son gelişmeler doğrultusunda kapsamlı olarak ele alınmıştır.

Keywords

Kromojenik besiyeri, florojenik besiyeri, Enterobacteriaceae, gıda mikrobiyolojisi

Chromogenic and fluorogenic media for members of Enterobacteriaceae in food microbiology

Abstract

Fast detection of microbiological risks is important for quality assurance in the food industry and public health. For this reason, several alternative methods have been developed to reduce the analysis period in food microbiology. Most of these methods are based on the utilization of chromogenic or fluorogenic substrates for the detection of specific enzyme activities of microorganisms. In food microbiology laboratories, use of media with chromogenic and fluorogenic substrates has continually increased due to cost, labor, and time savings features. This review discusses chromogenic and fluorogenic media for analysing members of Enterobacteriaceae, the most important family in food microbiology, in respect of recent devolopments. According to results of the latest studies, performance, advantage and disadvantage of chromogenic and fluorogenic media were evaluated

Keywords

Chromogenic medium, fluorogenic medium, Enterobacteriaceae, food microbiology

References

• 1. Manafi M. New developments in chromogenic and fluorogenic culture media. Int J Food Microbiol, 2000; 60: 205-18.
• 2. Manafi M. Fluorogenic and chromogenic substrates in culture media and identification tests. Int J Food Microbiol, 1996; 31: 45-58.
• 3. Gonzales RD, Tamagnini LM, Olmos PD, de Sousa GB. Evaluation of a chromogenic medium for total coliforms and Escherichia coli determination in ready-to-eat foods. Food Microbiol, 2002; 20: 601-4.
• 4. Doğan HB, Çakır İ, Başpınar E, Halkman AK. Comparison of LST + MUG broth technique and conventional method for the enumeration of Escherichia coli in foods. Lett Appl Microbiology, 2002; 34(4): 274-8.
• 5. Godsey JH, Matteo MR, Shen D, Tolman G, Gohike JR. Rapid identification of Enterobacteriaceae with microbial enzyme activity profiles. J Clin Microbiol, 1981; 13: 483-90.
• 6. Rice EW, Allen MJ, Brenner DJ, Edberg SC. Assay for ß-glucuronidase in species of the genus Escherichia and its application for drinking-water analysis. Appl Environ Microbiol, 1991; 57: 592-3.
• 7. Frampton EW, Restaino L. Methods for E. coli identification in food, water and clinical samples based on β-glucuronidase detection. J Appl Bacteriol, 1993; 74: 223-33.
• 8. Feng PCS, Hartman PA. Fluorogenic assays for immediate confirmation of Escherichia coli. Appl Environ Microbiol, 1982; 43(6): 1320-9.
• 9. Moberg LJ. Fluorogenic assay for rapid detection of Escherichia coli in food. Appl Environ Microbiol, 1985; 50: 1383-7.
• 10. Manafi M, Kneifel W, Bascomp S. Fluorogenic and chromogenic substrates used in bacterial diagnostics. Microbiol Rev, 1991; 55(3): 335-48.
• 11. Peterson EH, Nierman ML, Rude RA, Peeler JT. Comparison of AOAC method and fluorogenic method (MUG) assay for enumeration of E. coli in foods. J Food Sci, 1987, 52: 409-10.
• 12. Robison BJ. Evaluation of a fluorogenic assay for detection of Escherichia coli in foods. Appl Environ Microbiol, 1984; 48(2): 285-8.
• 13. Moberg LJ, Wagner MK, Kellen LA. Fluorogenic assay for rapid detection of Escherichia coli in chilled and frozen foods: collaborative study. J AOAC Int, 1988; 71(3): 589-602.
• 14. Poelma PL, Wilson CR, Andrews WH. Rapid fluorogenic enumeration of Escherichia coli in selected, naturally contaminated high moisture foods. J AOAC Int, 1987; 70: 991-3.
• 15. Health Protection Agency. National Standard Method, MSOP 31: Tryptone Bile X-Glucuronide (TBX) Agar. London, 2004.
• 16. Reinders RD, Bijker PGH. Veld JHJ, van Knapen F. Use of 8-hydroxyquinoline-β-D-glucuronide for presumptive identification of Shiga toxin-producing Escherichia coli O157. Lett Appl Microbiol, 2000; 30: 411-4.
• 17. Li F, Zhao C, Zhang W, Cui S, Meng J, Wu J, Zhang DY. Use of ramification amplification assay for detection of Escherichia coli O157:H7 and other E. coli Shiga toxin-producing strains. J Clin Microbiol, 2005; 43(12): 6086-90.
• 18. Manafi M, Kremsmaier B. Comparative evaluation of different chromogenicrfluorogenic media for detecting Escherichia coli O157:H7 in food. Int J Food Microbiol, 2001; 71: 257-62.
• 19. Health Protection Agency. National Standard Method MSOP 23: Cefixime tellurite sorbitol MacConkey (SMAC) agar. London, 2005.
• 20. Food and Drug Administration. Rapid methods for detecting foodborne pathogens. Bacteriological Analytical Manuel. Maryland, 2001.
• 21. Anonim. Rainbow agar O157 technical information, Biolog Inc. Hayward, 2003.
• 22. Anonim. Microbiology manuel, Merck KGaA, Darmstadt, 2006.
• 23. Restaino L, Frampton EW, Turner KM, Allison DRK. A chromogenic plating medium for isolating Escherichia coli O157:H7 from beef. Lett Appl Microbiol, 1999, 29: 26-30
• 24. Çakır İ. Koliform grup bakteriler ve E. coli, Gıda Mikrobiyolojisi ve Uygulamaları. Ankara Üniversitesi Ziraat Fakültesi Gıda Mühendisliği Bölümü Yayınları. Armoni Matbaacılık: Ankara, 1999
• 25. Ley AN, Bowers RJ, Wolfe S. Indoxyl-ß-Dglucuronide, a novel chromogenic reagent for the specific detection and enumeration of Escherichia coli in environmental samples. Can J Microbiol, 1988; 34: 690-3.
• 26. Suwansonthichai H, Rengpipat S. Enumeration of coliforms and Escherichia coli in frozen black tiger shrimp penaeus monodon by conventional and rapid methods. Int J Food Microbiol, 2003, 81(2): 113-21.
• 27. Orenga S, James AL, Manafi M, Perry JD, Pincus DH. Enzymatic substrates in microbiology. J Microbiol Methods, 2009, 79(2): 139-55.
• 28. Jasson V, Jacxsens L, Luning P, Rajkovic A, Uyttendaele M. Alternative microbial methods: An overview and selection criteria. Food Microbiol, 2010, 27: 710-30.
• 29. Torlak E, Akan İ, Gökmen M. Comparison of TEMPO EC and TBX medium for the enumeration of Escherichia coli in cheese. Lett Appl Microbiol, 2008, 47(6): 566-70.
• 30. Erol İ. Gıda Hijyeni ve Mikrobiyolojisi. 1. Baskı. Pozitif Matbaacılık: Ankara, 2007.
• 31. Food and Drug Administration. Salmonella. Bacteriological Analytical Manuel, Maryland, 2006.
• 32. Health Protection Agency. National Standard Method F 13: Detection of Salmonella species. London, 2007.
• 33. International Organization for Standardization. Microbiology of food and animal feeding stuffs - horizontal method for the detection of Salmonella spp. Cenevre, 2002.
• 34. Rall VLM, Rall R, Aragon LC, da Silva MG. Evaluation of three enrichment broths and five palting media for Salmonella detection in poultry. Braz J Microbiol, 2005;36: 147-50.
• 35. Perez JM, Cavalli P, Roure C, Renac R, Gille Y, Freydiere AM. Comparison of four chromogenic media and Hektoen agar for detection and presumptive identification of Salmonella strains in human stools. J Clin Microbiol, 2003;41(3): 1130-4.
• 36. Cooke VM, Miles RJ, Price RG, Richardson AC. A novel chromogenic ester agar medium for detection of Salmonellae. Appl Environ Microbiol, 1999; 65(2): 807-12.
• 37. Public Health Laboratory Service. Salmonella serotypes recorded in the PHLS Salmonella data set: January to December. p. 103, 216, 338, 444. Londra, 1997.
• 38. Cassar R, Cuschieri P. Comparison of Salmonella chromogenic medium with DCLS Agar for isolation of Salmonella species from stool specimens. J Clin Microbiol, 2003; 41(7): 3229-32.
• 39. Peery JD, Ford M, Taylor J, Jones AL, Freeman R, Gould FK. ABC Medium, a new chromogenic agar for selective isolation of Salmonella spp. J Clin Microbiol, 1999;37(3): 766-8.
• 40. Pignato S, Mario AM, Emanuele MC, Iannotta V, Caracappa S, Giammanco G. Evaluation of new culture media for rapid detection and isolation of Salmonella in foods. Appl Environ Microbiol, 1995; 61(5): 19996-9.
• 41. Gruenewald R, Henderson RW, Yappow S. Use of Rambach propylene glycol containing agar for identification of Salmonella spp. J Clin Microbiol, 1991; 29(10): 2354-6.
• 42. Kühn H, Wonde B, Rabsch W, Reissbrodt R. Evaluation of Rambach agar for detection of Salmonella subspecies I to VI. Appl Environ Microbiol, 1994;60(2): 749-51.
• 43. Dusch H, Altwegg M. Evaluation of five new plating media for isolation of Salmonella species. J Clin Microbiol, 1995;33(4):802-4.
• 44. Cawthorn DM, Botha S, Witthuhn Rc. Evaluation of different methods for the detection and identification of Enterobacter sakazakii isolated from South African infant formula milks and the processing environment. Int J Food Microbiol, 2008; 127: 129-38.
• 45. Iversena C, Drugganb P, Forsythea S. A selective differential medium for Enterobacter sakazakii, a preliminary study. Int J Food Microbiol, 2004; 96: 133-9.
• 46. Druggan P, Iversen C. Culture media for the isolation of Cronobacter spp. Int J Food Microbiol, 2009; 136: 169-78.