Akut Gastroenteritli Çocuklarda Human Bocavirus DNA Varlığının Araştırılması

Year 2017, Volume: 74 Issue: 4, 261 - 268, 01.12.2017

Serhat Sirekbasan Kenan Midilli Yasemin Akın Pelin Demirci Sevgi Ergin Arif Kaygusuz

Abstract

Amaç: Akut gastroenteritler, çocuklarda alt solunum yolu enfeksiyonlarından sonra en sık morbidite ve mortalite nedenidir. Beş yaşın altındaki çocuklarda dünyada her yıl bir milyar çocuk ishale yakalanmakta ve yaklaşık altı milyon çocuk ishal nedeni ile kaybedilmektedir. Gastroenterit etkenlerinin bilinmesi, doğru tanı ve etkin tedavi fırsatı sağlamasının yanı sıra antimikrobiyal tedavi gereken durumlarda antibiyotik seçimi için de yol gösterici olmaktadır. Bu çalışmada, hastanemize başvuran 0-5 yaş arası çocuklardaki akut gastroenteritlerde Human Bocavirus varlığı ve sıklığının araştırılması amaçlandı.Yöntem: Çalışmaya, hastanemize başvuran akut gastroenteritli 0-5 yaş arasındaki 101 çocuktan 42 kız, 59 erkek alınan dışkı örnekleri dahil edilmiştir. Human Bocavirus HBoV DNA’sı NP-1 gen bölgesine uygun primer dizilerinin kullanıldığı PCR yöntemi ile araştırılmıştır.Bulgular: İncelenen 101 örnekten 7 % 6,9 ’si HBoV pozitif olarak saptanmıstır. HBoV pozitif hastaların yaş ortalaması 1,5 1–2,5 yaş olup %71,4’ü iki yaş altıdır. 7 pozitif örnekten de ikisinde %28,6 birden fazla virus açısından HBoV ve Norovirus pozitiflik elde edilmiş ve eşzamanlı enfeksiyon varlığı düşünülmüştür. was considered. Conclusion: Studies conducted to investigate the role of HBoV in gastrointestinal system infections are importantfor treatment and epidemiological purposes. Further studies are required for the purpose of determining the role of HBoV in acute gastroenteritis infections. We believe that the present study of ours will contribute to the epidemiological studies since it is the very first research in this field

Keywords

İnsan Bocavirusu, gastroenterit, çocuk, polimeraz zincir reaksiyonu, dışkı

Investigation of Human Bocavirus DNA in children with acute gastroenteritis

Abstract

Objective: Acute gastroenteritis is the second frequent reason of morbidity and mortality after lower respiratory tract infections. One billion children below the age of five have diarrhea every year throughout the world, and nearly six million children are lost due to diarrhea. Knowing gastroenteritis factors enables physicians to make accurate and efficient diagnoses, and guides them to select the right antibiotic when the cases require antimicrobial treatment. In this study, the purpose is to examine the existence and frequency of Human Bocavirus in acute gastroenteritis in children between 0-5 years of age applying to our hospital.Methods: Stool samples taken from 101 children 42 female, 59 male between the age of 0-5 with acute gastroenteritis were included in the study. The DNA of the Human Bocavirus HBoV was investigated with the PCR Method in which proper primary series were used for NP-1 gen area. Results: Seven 6,9% of the 101 samples that were examined in the scope of the study were determined to be HBoV positive. The mean age of the patients who were HBoV positive was 1,5 1-2,5 years of age ; and 71,4% were below the age of two. In two of the 7 positive samples 28,6% positivity for more than more virus HBoV and Norovirus was determined, and simultaneous infection was considered. Conclusion: Studies conducted to investigate the role of HBoV in gastrointestinal system infections are importantfor treatment and epidemiological purposes. Further studies are required for the purpose of determining the role of HBoV in acute gastroenteritis infections. We believe that the present study of ours will contribute to the epidemiological studies since it is the very first research in this field

Keywords

Human Bocavirus, gastroenteritis, child, polymerase chain reaction, stool

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