Nrf2 inhibitörü Brusatol, sıçanlarda anti-inflamasyon ve anti-oksidatif stres yoluyla çekal ligasyonu ve delinmeye bağlı akciğer hasarını iyileştirir

Year 2020, Volume: 77 Issue: 4, 467 - 476, 01.12.2020

Ersen Eraslan Ayhan Tanyeli Mustafa Can Güler Fazile Nur Ekinci Akdemir Tuncer Nacar Ömer Topdağı Elif Polat

Abstract

Amaç: Bu çalışmanın amacı, brusatolün sıçanlarda çekal ligasyon ve delinme CLD modelinin neden olduğu akciğer hasarı üzerine olası yararlı etkilerinin incelenmesidir.Yöntem: Bu çalışmada 32 adet Sprague Dawley erkek sıçan 4 rastgele gruba ayrıldı. Gruplar; grup 1 sham , grup 2 CLD , grup 3 DMSO ve grup 4 0.5 mg/ml brusatol olarak programlandı. Grup 1’de karın bölgesine orta hatta dikey insizyon uygulandı ve CLD modeli oluşturmadan tekrar kapatıldı. Grup 2’de 18 saat CLD yapıldı. Grup 3’e, %1 DMSO 10 gün boyunca iki günde bir periton içine 0.3 ml uygulandı. Son uygulama CLD’den 30 dakika önce yapıldı. Grup 4’e brusatol 10 gün boyunca iki günde bir periton içine 0.5 mg/ml olarak uygulandı. Son uygulama CLD’den 30 dakika önce yapıldı. CLD bittikten sonra sıçanlar sakrifiye edildi ve akciğer dokuları çıkarıldı. Bulgular: MDA, MPO, OSI, TOS, TNF-α ve IL-1β değerleri grup 2 ve 3’te, grup 1’e kıyasla anlamlı derecede yükselirken, SOD ve TAS değerleri azaldı. Antioksidan enzim aktivitesindeki artışın aksine; MDA seviyesi, MPO aktivitesi, TOS, OSI, TNF-α ve IL-1β değerleri grup 4’te grup 2 ve 3’e göre önemli ölçüde azaldı. Sonuç: Sıçanlarda CLD kaynaklı akciğer hasarına karşı brusatol etkin rol oynayabilir

Keywords

Brusatol, oksidatif stres, sitokin, çekal ligasyon ve delinme, akciğer

Nrf2 Inhibitor Brusatol Ameliorates Cecal Ligation and Puncture-induced Lung Injury in Rats via Anti-inflammation and Anti-oxidative Stress

Abstract

Objective: The aim of this study is to investigate the possible beneficial effects of brusatol on lung injury caused by cecal ligation and puncture CLP model in rats. Methods: In this study, 32 Sprague Dawley male rats were divided into 4 random groups. The groups were programmed as group 1 sham , group 2 CLP , group 3 DMSO , and group 4 0.5 mg/ml brusatol . In group 1, a midline vertical incision was applied to the abdominal region and closed again without forming a CLP model. Group 2 performed CLP for 18 hours. In group 3, 1% DMSO was administered intraperitoneally 0.3 ml once every two days for 10 days. The last application was made 30 minutes before CLP. In group 4, brusatol was administered intraperitoneally at 0.5 mg/ml once every two days for 10 days. The last application was made 30 minutes before CLP. After CLP was over, the rats were sacrificed and lung tissues were removed.Results: MDA, MPO, OSI, TOS, TNF-α and IL-1β values increased significantly in groups 2 and 3 compared to group 1, while SOD and TAS values decreased. Unlike the seviyesi, MPO aktivitesi, TOS, OSI, TNF-α ve IL-1β değerleri grup 4’te grup 2 ve 3’e göre önemli ölçüde azaldı. Sonuç: Sıçanlarda CLD kaynaklı akciğer hasarına karşı brusatol etkin rol oynayabilir

Keywords

Brusatol, oxidative stress, cytokine, cecal ligation and puncture, lung

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