Kafeinin embriyonel böbrek gelişimine olan etkisine karşı melatoninin koruyucu rolü

Year 2020, Volume: 77 Issue: 1, 51 - 58, 01.03.2020

Seher Yılmaz Ayşe Yeşim Göçmen Arda Kaan Üner Enes Akyüz Adem Tokpınar

Abstract

Amaç: Oksidatif stres bozukluklarının en büyük sebeplerinden biridir. Ayrıca, melatonin vücutta oksidatif stresi azaltması nedeniyle kafeinin fetal gelişime olan olumsuz etkilerine karşı potansiyel bir tedavi yöntemi olarak görülmektedir. Bu çalışmada; kafeinin fetal böbrek gelişimine olan teratojenik etkisi ve melatonin maddesinin bu teratojeniteye karşı koruyuculuğunu biyokimyasal parametreler üzerinden incelemeyi hedeflenmiştir. TAS değeri kontrol grubunda 0,49±0,02, yüksek doz kafein grubunda 0,14±0.00 olduğu görülmüştür. Ayrıca total oksidan kapasite TOS , oksidatif stres indeksi OSI , glutatyon GSH , glutatyon disülfit GSSG , süperoksit dismutaz SOD , tiyobarbitürik asit reaktif maddeler TBARS , Kalsiyum Ca , D vitamini ve GSH/GSSG seviyeleri incelenmiştir p

Keywords

Kafein, sıçan, melatonin, böbrek

The protective role of melatonin against the effect of caffeine on embryonic kidney

Abstract

Objective: Oxidative stress is one of the major causes of embryonal developmental disorders. In addition, melatonin reduces oxidative stress in the body and is known as a potential treatment for adverse effects of caffeine on fetal development. In this study, we aimed to investigate the teratogenic effect of caffeine on fetal kidney development and the protection of melatonin against the teratogenicity in terms of biochemical parameters.Methods: 5-7 months old Wistar-Albino rats n=24 weighing 180-220 g were divided into seven groups for control, caffeine, melatonin, and caffeine+melatonin co-injection . Control group: Serum physiological SF as 1 mL/kg intraperitoneal i.p. , Sham group: 0.1 ml hanks as i.p., Low dose caffeine group: 30 mg/kg caffeine as gavage, Low dose caffeine+melatonin group: 30 mg/kg dose gavage with 10 mg/kg melatonin as i.p., High dose caffeine group: 60 mg/kg caffeine as gavage, High dose caffeine+melatonin group: 60 mg/kg dose gavage with 10 mg/kg melatonin as i.p., and Melatonin group: 10 mg/kg melatonin as i.p.Results: Total antioxidant status TAS values were 0.49±0.02 in the control group and 0.14±0.00 in the high TAS değeri kontrol grubunda 0,49±0,02, yüksek doz kafein grubunda 0,14±0.00 olduğu görülmüştür. Ayrıca total oksidan kapasite TOS , oksidatif stres indeksi OSI , glutatyon GSH , glutatyon disülfit GSSG , süperoksit dismutaz SOD , tiyobarbitürik asit reaktif maddeler TBARS , Kalsiyum Ca , D vitamini ve GSH/GSSG seviyeleri incelenmiştir p

Keywords

Caffeine, rat, melatonin, kidney

References

• 1. Prakash R, Tanuja S, Gokulnatha. Review of oxidative stress in relevance to uremia. Clin Quer: Nephrol, 2012; 1 (3): 215-21.
• 2. Ling, XC, Ko-Lin K. Oxidative stress in chronic kidney disease. Ren ReplaceTher, 2018; 4 (1): 53.
• 3. Sultana Z, Maiti K, Aitken J, Morris J, Dedman L, Smith R. Oxidative stress, placental ageing‐related pathologies and adverse pregnancy outcomes. Am J Reprod Immunol, 2017; 77 (5): e12653.
• 4. Knight CA, Knight I, Mitchell DC, Zepp JE. Beverage caffeine intake in US consumers and subpopulations of interest: estimates from the Share of Intake Panel survey. Food Chem Toxicol, 2004; 42 (12): 1923-30.
• 5. Xu F, Liu P, Pekar JJ, Lu H. Does acute caffeine ingestion alter brain metabolism in young adults? Neuroimage, 2015; 110: 39-47.
• 6. Ng YP, Or TCT, Ip NY. Plant alkaloids as drug leads for Alzheimer’s disease. Neurochem Int, 2015; 89: 260-70.
• 7. Fox GP, Wu A, Yiran L, Force L. Variation in caffeine concentration in single coffee beans. J Agr Food Chem, 2013; 61 (45): 10772-8.
• 8. Nehlig A, Jean-Luc D, Gérard D. Caffeine and the central nervous system: mechanisms of action, biochemical, metabolic and psychostimulant effects. Brain Res Rev, 1992; 17 (2): 139-70.
• 9. Grosso LM, Michael BB. Caffeine metabolism, genetics, and perinatal outcomes: a review of exposure assessment considerations during pregnancy. Ann Epidemiol, 2005; 15 (6): 460-6.
• 10. Al-Basher GI, Aljabal H, Almeer RS, Allam AA, Mahmoud AM. Perinatal exposure to energy drink induces oxidative damage in the liver, kidney and brain, and behavioral alterations in mice offspring. Biomed Pharmacother, 2018; 102: 798-811.
• 11. Matijasevich A, Barros FC, Santos IS, Yemini A. Maternal caffeine consumption and fetal death: a case–control study in Uruguay. Paediatr Perinat Epidemiol, 2006; 20 (2): 100-9.
• 12. Li J, Zhao H, Song JM, Zhang J, Tang YL, Xin CM. A meta-analysis of risk of pregnancy loss and caffeine and coffee consumption during pregnancy. Int J Gynecol Obstet, 2015; 130 (2): 116-22.
• 13. Weng X, Roxana O, De-Kun L. Maternal caffeine consumption during pregnancy and the risk of miscarriage: a prospective cohort study. Am J Obstet Gynecol, 2008; 198 (3): 279-e1-8.
• 14. Dollins AB, Zhdanova IV, Wurtman RJ, Lynch HJ, Deng MH. Effect of inducing nocturnal serum melatonin concentrations in daytime on sleep, mood, body temperature, and performance. Proc Natl Acad Sci, 1994; 91 (5): 1824-8.
• 15. Meca R, Balbo BE, Ormanji MS, Fonseca JM, Iannuzzi LR, Santana CE, et al. Caffeine accelerates cystic kidney disease in a Pkd1-deficient mouse model. Cell Physiol Biochem, 2019; 52: 1061-74.
• 16. Nisari M, Yılmaz S, Göçmen AY, Karataş E, Özge A. The protective effect of caffeine and melatonin on antioxidant enzymes in rat fetal lung tissues. J Surg Med, 2019; 3 (11): 805-8.
• 17. Czerska M, Mikolajewska K, Zielinski M, Gromadzinska J, Wasowicz W. Today’s oxidative stress markers, Medycyna Pracy, 2015; 66(3):393– 405.
• 18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J BiolChem, 1951; 193: 265-75.
• 19. Erel OA. Novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem, 2004; 37 (4): 277-85.
• 20. Galano A, Dun XT, Russel JR. Melatonin as a natural ally against oxidative stress: a physicochemical examination. J Pineal Res, 2011; 51 (1): 1-16.
• 21. Tan DX, Manchester LC, Hardeland R, Lopez‐Burillo S, Mayo JC, Sainz RM, et al. Melatonin: a hormone, a tissue factor, an autocoid, a paracoid, and an antioxidant vitamin. J Pineal Res, 2003; 34 (1): 75- 8.
• 22. Groenman F, Sharon U, Martin P. The molecular basis for abnormal human lung development. Neonatology, 2005; 87 (3): 164-77.
• 23. Morabito R, Alessia R, Angela M. Melatonin Protects Band 3 Protein in Human Erythrocytes against H2O2-Induced Oxidative Stress. Molecules, 2019; 24 (15): 2741.
• 24. Radovic M, Ristic L, Krtinic D, Rancic M, Nickovic V, Vujnovic ZZ, et al. Melatonin treatment prevents carbon tetrachloride-induced acute lung injury in rats by mitigating tissue antioxidant capacity and inflammatory response. Bratisl Med J, 2019 120 (7): 527-31.
• 25. Reiter RJ, Rosales CS, Tan DX, Jou MJ, Galano A, Xu B. Melatonin as a mitochondria-targeted antioxidant: one of evolution’s best ideas. Cell Mol Life Sci, 2017; 74 (21): 3863-81.
• 26. Lee JY, Li S, Shin NE, Na Q, Dong, J, Jia B, et al. Melatonin for prevention of placental malperfusion and fetal compromise associated with intrauterine inflammation‐induced oxidative stress in a mouse model. J Pineal Res, 2019; e12591.
• 27. Hsu CN, Huang LT, Tain YL. Perinatal use of melatonin for offspring health: focus on cardiovascular and neurological diseases. Int J Mol Sci, 2019; 20 (22): 5681.