mtDNA COI and cyt b Analysis of Carassius gibelio (Bloch, 1782) Living in Atatürk Dam Lake (Turkey)

Year 2022, Volume: 6 Issue: 2, 45 - 50, 09.09.2022

Arif Parmaksız , Aynur Demir

https://doi.org/10.26650/tjbc.1112782

Abstract

Objective: The aim of this study is to compare the C. gibelio species living in Atatürk Dam Lake with the sequences in the gene bank based on the sequences of the mtDNA COI and Cyt b gene regions and to perform phylogenetic analyzes.
Material and Methods: Individuals of the target species living in the Bozova region of Atatürk Dam Lake were randomly selected from fishermen and purchased. Total DNA isolation was performed from muscle tissue using the GeneJET Genomic DNA Purification Kit (Thermo Scientific) according to the protocol instructions. Then, using specific primers of mtDNA COI and Cyt-b regions, the relevant regions were amplified by PCR method. The obtained PCR products were carried out in Agarose gel and the samples forming the product were selected and sent to the commercial firm as service procurement and sequence analysis was performed with the 3500 XL Genetic Analyzer device.
Results: Sequences of 620 and 580 bp in length were obtained for the mtDNA COI and cyt b gene regions, respectively. Although the same haplotype was detected for the mtDNA COI region with individuals living in Turkey before, a new haplotype was detected for the mtDNA cyt b gene region that was not previously defined.
Conclusion: Sequence analyzes of mtDNA COI and cyt b gene regions of individuals belonging to the C. gibelio species living in Atatürk Dam Lake were performed for the first time in this study and compared with the data in the gene bank.

Keywords

Atatürk Dam Lake, Carassius gibelio, mtDNA, COI, cyt b

Supporting Institution

Harran university (HUBAK)

Project Number

220

Atatürk Baraj Gölü’nde (Türkiye) Yaşayan Carassius gibelio (Bloch, 1782) türünün mtDNA COI ve cyt b Analizi

Abstract

Amaç: Bu çalışmanın amacı, Atatürk Baraj Gölü’nde yaşayan C. gibelio türünün mtDNA COI ve Cyt b gen bölgelerine ait sekanslara dayalı olarak gen bankasındaki sekanslarla karşılaştırılması ve filogenetik analizlerin yapılmasıdır.
Materyal ve Yöntem: Atatürk Baraj Gölü Bozova bölgesinde yaşayan hedef türe ait bireyler balıkçılardan rastgele seçilerek satın alınmıştır. Total DNA izolasyonu, GeneJET Genomic DNA Purification Kit (Thermo Scientific) kullanılarak kas dokusundan protokol talimatları doğrultusunda yapılmıştır. Daha sonra mtDNA COI ve Cyt-b bölgelerine ait özgül primerler kullanılarak ilgili bölgeler PCR yöntemi ile çoğaltılmıştır. Elde edilen PCR ürünleri Agaroz jelde yürütülmüş ve ürün oluşturan örnekler seçilerek hizmet alımı şeklinde ticari firmaya gönderilmiş ve 3500 XL Genetic Analyzer cihazı ile dizi analizi yaptırılmıştır.
Bulgular: mtDNA COI ve cyt b gen bölgeleri için sırayla 620 ve 580 bp uzunlukta sekanslar elde edilmiştir. mtDNA COI bölgesi için daha önce Türkiye’de yaşayan bireylerle aynı haplotipler tespit edilmesine rağmen mtDNA Cyt b gen bölgesi için daha önce tanımlanmayan yeni bir haplotip tespit edilmiştir.
Sonuç: Atatürk Baraj Gölü’nde yaşayan C. gibelio türüne ait bireylerin mtDNA COI ve Cyt b gen bölgelerine ait sekans analizleri ilk defa bu çalışmada yapılmış ve gen bankasındaki verilerle karşılaştırılmıştır.

Keywords

Atatürk Baraj Gölü, Carassius gibelio, mtDNA, COI, cyt b

Project Number

220

References

• Ağdamar, S., & Tarkan, A. S. (2019). High genetic diversity in an invasive freshwater fish species, Carassius gibelio, suggests establishment success at the frontier between native and invasive ranges. Zoologischer Anzeiger, 283, 192-200. google scholar
• Ağdamar, S. (2017). Türkiye iç sularında dağılım gösteren istilacı balıklardan gümüşi havuz balığı (Carassius gibelio Bloch, 1782) populasyonlarının genetik çeşitliliğinin belirlenmesi. Doktora Tezi. Muğla Sıtkı Koçman Üniversitesi, Fen Bilimleri Enstitüsü. Su Ürünleri Mühendisliği Ana Bilim Dalı. google scholar
• Briolay, J., Nicols Galtier, N., Brito, R. M. & Bouvet, Y. (1998). Molecular phylogeny of Cyprinidae inferred from cytochrome b DNA sequences. Molecular Phylogenetics and Evolution, 9(1), 100-108. google scholar
• Cheng, L., Chang, Y. M., Lu, C. Y., Cao, D. C. & Sun, X. W. (2012). DNA barcoding and species and subspecies classification within genus Carassius. Zoological Research 33: 463-472. google scholar
• Darabi, A. R., Kashan, N., Fayazi, J., Aminafshar, M. & Chamani, M. (2014). İnvestigation of phylogenetic relationship among two Barbus species (Cyprinidae) populations with mitochondrial DNA using PCR sequencing. IJBPAS, 4(2), 302-311. google scholar
• Erdem, Y., Samur, M., & Özdemir, S. (2014). İç sularda istilacı balık türleriyle mücadelede seçici avlama yöntemlerinin etkinliği. Aquatic Sciences and Engineering, 29(2), 49-63. google scholar
• Jackson, M. C., Wasserman, R. J., Grey, J., Ricciardi, A., Dick, J. T. & Alexander, M. E. (2017). Novel and disrupted trophic links following invasion in freshwater ecosystems. İn Advances in Ecological Research (Vol. 57, pp. 55-97). Academic Press google scholar
• Gozlan, R. E., Britton, J. R., Cowx, İ. & Copp, G. H. (2010). Current knowledge on non-native freshwater fish introductions. Journal of fish biology, 76(4), 751-786. google scholar
• Komiyama, T., Kobayashi, H., Tateno, Y., İnoko, H., Gojobori, T. & İkeo, K. (2009). An evolutionary origin and selection process of goldfish. Gene, 430(1-2), 5-11. google scholar
• Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35, 1547-1549. google scholar
• Leung, B., Lodge, D. M., Finnoff, D., Shogren, J. F., Lewis, M. A. & Lamberti, G. (2002). An ounce of prevention or a pound of cure: bioeconomic risk analysis of invasive species. Proceedings of the Royal Society of London. Series B: Biological Sciences, 269(1508), 2407-2413. google scholar
• Mollot, G., Pantel, J. H. & Romanuk, T. N. (2017). The effects of invasive species on the decline in species richness: a global meta-analysis. İn Advances in ecological research (Vol. 56, pp. 61-83). Academic Press. google scholar
• Oymak, S. A. (2000). Atatürk Baraj Gölü’nde yaşayan Chondrostoma regium (Heckel, 1843) un büyüme özellikleri. Turkish Journal of Zoology, 24(supp), 41-50. google scholar
• Oymak, S. A., Erhan, Ü. N. L. Ü., Parmaksiz, A. & Doğan, N. (2011). A study on the age, growth and reproduction of Aspius vorax (Heckel, 1843)(Cyprinidae) in Atatürk Dam Lake (Euphrates River), Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 11(2). google scholar
• Parmaksız, A., Oymak, S. A., Dogan, N., Naim, D. M. & Unlu, E. (2017). Reproductive characteristics of an invasive species Carassius gibelio (Bloch, 1782) in Ataturk Dam Lake, Turkey. Indian Journal of Fisheries, 64(4), 28-33. google scholar
• Parmaksız, A., & Eskici, H. K. (2018). Genetic variation of yellow barbell (Carasobarbus luteus (Heckel, 1843)) from four populations using mitochondrial DNA COİ gene sequences. Applied Ecology and Environmental Research, 16(2), 1673-1682. google scholar
• Parmaksız, A., Şeker, Ö. (2018). Genetic diversity of the endemic species shabbout (Arabibarbus grypus (Heckel, 1843)) based on partial cytochrome b sequences of mitochondrial DNA. Aquatic Research, 1(3), 103-109. google scholar
• Parmaksız, A., Korkmaz, E., Ulusal, D. & Doğan, N. (2022a). Phylogenetic analysis of Luciobarbus Heckel, 1843 and Barbus Cuvier & Cloquet, 1816 species in the Euphrates River (Turkey) based on mtDNA COI gene sequences. Aquatic Research, 5(2), 129-135. google scholar
• Parmaksız, A., Necati, E., Kadir, E. & Koyuncu, İ. (2022b). Fırat ve Dicle nehirlerinde yaşayan Carassius gibelio (Bloch, 1782) türünün aminoasit profilinin araştırılması. Turkish Journal of Bioscience and Collections, 6(1). google scholar
• Sheraliev, B., & Peng, Z. (2021). Molecular diversity of Uzbekistan’s fishes assessed with DNA barcoding. Scientific Reports, 11(1), 1-12. google scholar
• Tarkan, A. S., Copp, G. H., Top, N., Özdemir, N., Önsoy, B., Bilge, G., ... & Saç, G. (2012a). Are introduced gibel carp Carassius gibelio in Turkey more invasive in artificial than in natural waters?. Fisheries Management and Ecology, 19(2), 178-187. google scholar
• Tarkan, A. S., Gaygusuz, Ö., Gürsoy Gaygusuz, Ç., Saç, G. & Copp, G. H. (2012b). Circumstantial evidence of gibel carp, Carassius gibelio, reproductive competition exerted on native fish species in a mesotrophic reservoir. Fisheries Management and Ecology, 19(2), 167-177. google scholar
• Tsipas, G., Tsiamis, G., Vidalis, K., & Bourtzis, K. (2009). Genetic differentiation among Greek lake populations of Carassius gibelio and Cyprinus carpio carpio. Genetica, 136(3), 491-500. google scholar
• Uğurlu, S., & Polat, N. (2007). Samsun ili tatlı su kaynaklarında yaşayan egzotik balık türleri. Journal of FisheriesSciences. com, 1(3), 139-151. google scholar
• Yerli, S. V., Mangıt, F., Emiroğlu, Ö., Yeğen, V., Uysal, R., Ünlü, E., ... & Zengin, M. (2014). Distribution of invasive Carassius gibelio (bloch, 1782) (Teleostei: Cyprinidae) in Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 14(2), 581-590. google scholar