The relationship between single gene polymorphism and response to cisplatin and 5-FU treatment in patients with head and neck cancer

Abstract

Aim: Head and neck cancers are the sixth most common type of cancer worldwide. The treatment process of head and neck cancers is classified as chemotherapy or chemoradiotherapy. In this study, the relationship of ERCC1, XRCC1 and MTHFR genes with treatment response was investigated. Material and Methods: In the study, 5 ml of blood was collected from the patients to investigate single nucleotide polymorphism, DNA was isolated and investigated by pyrosequencing method. Results: Patients were evaluated according to RECIST criteria; head and neck computed tomography scans were performed before treatment (4 weeks) and after every three cycles. The overall response rate (RR) was 10 (25%) PD, 7 (17.5%) SD, 9 (22.5%) PR, and 14 (35%) CR. Of the patients who presented with at least one polymorphic variant, four had PD, 3 had SD, 3 had PR and 1 had CR. Conclusion: In this study, the clinical behaviour of a group of head and neck carcinoma patients was retrospectively evaluated for association with three single nucleotide polymorphisms. These included C8092A in the ERCC1 gene, G28152A in the XRCC1 gene, and C677T and A1298C in the MTHFR gene.

Keywords

• Head and neck cancer
• SNP
• cisplatine
• 5-FU

Baş boyun kanserli hastalarda tek gen polimorfizmi ile sisplatin ve 5-FU tedavisine yanıt arasındaki ilişki

Abstract

Amaç: Baş ve boyun kanserleri dünya çapında en sık görülen altıncı kanser türüdür. Baş ve boyun kanserlerinin tedavi süreci kemoterapi veya kemoradyoterapi olarak sınıflandırılır. Bu çalışmada ERCC1, XRCC1 ve MTHFR genlerinin tedavi ile yanıt ilişkisi araştırıldı. Gereç ve Yöntemler: Çalışmada tek nükleotid polimorfizmini araştırmak amacıyla hastalardan 5 ml kan alınarak DNA izole edildi ve pirosequencing yöntemiyle araştırıldı. Bulgular: Hastalar RECIST kriterlerine göre değerlendirildi; tedaviden önce (4 hafta) ve her üç siklustan sonra baş ve boyun bilgisayarlı tomografi taramaları yapıldı. Genel yanıt oranı (RR) 10 (%25) PD, 7 (%17,5) SD, 9 (%22,5) PR ve 14 (%35) CR idi. En az bir polimorfik varyantı olan hastaların dördünde PD, 3'ünde SD, 3'ünde PR ve 1'inde CR vardı. Sonuçlar: Bu çalışmada bir grup baş boyun karsinomu hastasının klinik davranışı, üç tek nükleotid polimorfizmi ile ilişki açısından retrospektif olarak değerlendirildi. Bunlar arasında ERCC1 geninde C8092A, XRCC1 geninde G28152A ve MTHFR geninde C677T ve A1298C yer alıyordu.

Keywords

• Baş ve boyun kanseri
• SNP
• cisplatin
• 5-FU

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