Lökositoklastik vaskülitli hastalarda serum sCD163 ve çeşitli hastalık parametreleriyle ilişkisi

Abstract

Amaç: Bu çalışmada lökositoklastik vaskülit (LCV) tanısı almış hastalarda serum solubl CD163 (sCD163) düzeylerinin araştırılması ve bu biyobelirteç ile klinik, laboratuvar ve hastalık şiddeti arasındaki ilişkinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntemler: Çalışmamıza 43 LCV hastası ve 45 sağlıklı kontrol dahil edildi. Serum sCD163 düzeyleri ELISA ile ölçüldü. Gruplar arasındaki sCD163 düzeyleri arasındaki farklar, hastalıkla korelasyonları ve bulgular ile yerleşim yeri arasındaki ilişki ROC ve çok değişkenli analizler kullanılarak değerlendirildi. Bulgular: CRP, ESR, WBC, PLT, nötrofil sayısı, VKİ ve serum sCD163 düzeyleri LCV grubunda kontrol grubuna göre anlamlı derecede yüksek, HGB ve RBC düzeyleri ise daha düşüktü (p<0,05). sCD163 düzeyleri CRP, ESR ve diğer inflamatuvar parametrelerle pozitif, HGB ve RBC ile negatif korelasyon gösterdi. ROC analizi, sCD163 belirteci için %100 duyarlılık ve özgüllük ortaya koydu ve AUC değeri 1,00 idi. Ancak, lojistik regresyon analizi sCD163'ü anlamlı bir bağımsız öngörücü olarak belirlemedi. Çok değişkenli PCA ve PLS-DA analizleri, hasta ve kontrol grupları arasında anlamlı bir fark ortaya koydu ve sCD163 en anlamlı ayırt edici değişkendi. Sonuçlar: Çalışmamız, serum sCD163 düzeylerinin LCV'li hastalarda anlamlı şekilde arttığını ve inflamatuvar aktivite ile güçlü bir korelasyon gösterdiğini ortaya koydu. sCD163 tek başına hastalığın şiddetini tahmin etmek için yeterli olmasa da, yüksek tanısal doğruluğu onu LCV tanısında klinisyenler için değerli bir biyobelirteç haline getirebilir. Bulgularımız, makrofaj aktivasyonunun LCV hastalığının patogenezinde bir rolü olduğunu düşündüren verilerle tutarlıdır.

Keywords

• Biyobelirteç
• CD163
• inflamasyon
• lökositoklastik vaskülit

Serum sCD163 in patients with leukocytoclastic vasculitis and its relationship with various disease parameters

Abstract

Aim: This study aimed to investigate serum soluble CD163 (sCD163) levels in patients diagnosed with leukocytoclastic vasculitis (LCV) and evaluate the relationship between this biomarker and clinical, laboratory, and disease severity. Material and Methods: Forty-three patients with LCV and 45 healthy controls were included in our study. Serum sCD163 levels were measured using ELISA. The differences in sCD163 levels between groups, their correlation with disease, and the relationship between the findings and location were evaluated using ROC and multivariate analyses. Results: CRP, ESR, WBC, PLT, neutrophil count, BMI, and serum sCD163 levels were significantly higher in the LCV group than in the control group, whereas HGB and RBC levels were lower (p<0.05). sCD163 levels were positively correlated with CRP, ESR, and other inflammatory parameters and negatively correlated with HGB and RBC. ROC analysis revealed 100% sensitivity and specificity for the sCD163 marker, with an AUC of 1.00. However, logistic regression analysis did not identify sCD163 as a significant independent predictor. Multivariate PCA and PLS-DA analyses revealed a significant difference between the patient and control groups, with sCD163 being the most significant distinguishing variable. Conclusion: Our study revealed that serum sCD163 levels were significantly increased in patients with LCV and were strongly correlated with inflammatory activity. Although sCD163 alone is not sufficient to predict disease severity, its high diagnostic accuracy may make it a valuable biomarker for clinicians in the diagnosis of LCV. Our findings are consistent with data suggesting a role for macrophage activation in the pathogenesis of LCV disease.

Keywords

• Biomarker
• CD163
• inflammation
• leukocytoclastic vasculitis

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