Importance of neutrophil gelatinase-associated lipocalin and cystatin C in early diagnosis of cardiopulmonary bypass associated acute kidney injury in adult patients

Abstract

Aim: The development of acute kidney injury (AKI) after cardiopulmonary bypass (CPB) is associated with increased rates of mortality and morbidity. The identification of reliable biomarkers that allow earlier diagnosis of AKI in the post-operative period may increase the success of therapeutic interventions. The aim of our present study was to measure neutrophil gelatinase-associated lipocalin (NGAL) and cystatin C (Cys C) levels and to test the accuracy and reliability as an early biomarker.

Material and Methods: The study was designed as a prospective observational study, comparing creatinine, NGAL, and Cys C levels in patients who develop AKI or not after heart surgery using CPB. Forty-three patients met the inclusion criteria. We obtained blood samples for NGAL and Cys C at two predetermined time-points (6 and 24 hours) after the initiation of CPB. 

Results: According to the AKIN classification, 8 patients (44%) developed AKI (AKI1 groups) and 35 (56%) did not (non-AKI). The areas under the receiver operating characteristic curve (AUCROC) values were calculated for all parameters. AUC_ROC value was 0.81 (95% CI: 0, 63 to 1, 00) for 6^th hours NGAL and 0.867 (95% CI: 0, 00-1, 00, p: 0.004) for 24^th Cys C. Therefore, NGAL measured in 6^th hours and 24^th Cys C, have diagnostic value for early diagnosis of acute kidney injury.

Conclusion: Based on our study, NGAL levels measured in 6th hour, and Cys C levels measured in the 24th hour have high predictive value for early diagnosis for CPB-ABH. 

Keywords

• cardiopulmonary bypass,acute kidney injury,neutrophil gelatinase-associated lipocalin,cystatin C

Erişkin hastalarda kardiyopulmoner bypass sonrası gelişen akut böbrek hasarının erken tanısında neutrophil gelatinase-associated lipocalin ve sistatin C’nin önemi

Abstract

Amaç: Kardiyopulmoner bypass sonrası oluşan akut böbrek hasarı (CPB-ABH) mortalite ve morbidite artışı ile ilişkilidir. Postoperatif dönemde ABH erken tanısını sağlayacak biyobelirteçler tedavi başarısını artırabilir. Bu çalışmanın amacı neutrophil gelatinase-associated lipocalin (NGAL) ve sistatin C (Cys C) seviyelerini ölçmek ve erken biyobelirteç olarak doğruluk ve güvenilirliği test etmektir.

Gereç ve Yöntemler: Çalışmamız CPB kullanılarak uygulanan kalp cerrahisi sonrası ABH gelişen hastalarda kreatinin, NGAL ve Cys C düzeylerini karşılaştıran prospektif gözlemsel bir çalışmadır. Kırk üç hasta çalışmaya dâhil edildi. CPB'nin başlatılmasından sonra, önceden belirlenmiş iki zaman noktasında (6 ve 24 saat) NGAL ve Cys C için kan örnekleri alındı.

Bulgular: AKIN sınıflamasına göre, 8 hasta (% 44) ABH gelişti, 35 (% 56) hastada ABH gözlenmedi. Tüm parametreler için işletim karakteristiği eğrisi altında kalan alan (AUC_ROC) hesaplandı. AUC_ROC değeri 6.saat NGAL için 0,81 (%95 CI:0,63-1,0 p=006) ve 24. Cys C. için 0,867 (95% CI: 0,00-1,00 p: 0,004) idi. Bu nedenle, 6. saat NGAL ve 24. saat Cys C değerleri ABH teşhisi için tanısal değere sahiptir.

Sonuç: Çalışmamıza göre, 6. saatte NGAL ve 24. saatte Cys C düzeyleri, CPB-ABH erken teşhisi için güçlü bir tanısal değere sahiptir. 

Keywords

• Kardiyopulmoner Bypass,akut böbrek hasarı,neutrophil gelatinase-associated lipocalin,sistatin C

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