N-asetil sistein alt ekstremite iskemi/ reperfüzyonu tarafından indüklenen feroptosise bağlı akciğer hasarını azaltır

Year 2020, Volume: 11 Issue: 4, 288 - 293, 30.09.2020

Naim Boran Tümer

https://doi.org/10.18663/tjcl.769961

Cited By: 2

Abstract

Amaç: Bu çalışma, N-asetil sistein ön tedavisinin, alt ekstremite iskemisi ve reperfüzyonun neden olduğu ferroptoza bağlı akciğer hasarının önlenmesi üzerindeki etkisini analiz etmeyi amaçlamıştır.
Gereç ve Yöntemler: 350-400 g ağırlığında on sekiz erkek Sprague-Dawley sıçanı üç gruba randomize edildi. İskemik dönemden önce intraperitonal olarak 150 mg / kg N-Asetil Sistein veya normal salin 0.1 ml / kg verildi. Kontrol ve çalışma gruplarında, aort infrarenal düzeyde klemlenerek 2 saat boyunca I/R ve sonrasında 4 saat reperfüzyon indüklendi. Üçüncü gruba sham grubu olarak kullanıldı. Hayvanlarının hayatı sonlandırıldıktan sonra, histopatolojik ve biyokimyasal analiz için akciğerleri çıkarıldı.
Bulgular: Doku Malondialdehit (MDA) ve glutatyon (GSH) seviyeleri arasında istatistiksel olarak anlamlı fark tespit edildi. Kontrol grubunda, MDA düzeyleri ve GSH düzeyleri, ferroptoza bağlı akciğer hasarı gösteren sham grubuna kıyasla önemli ölçüde arttı. Bununla birlikte, NAC alan grupta MDA seviyesi düşerken koruyucu bir etki olarak GSH seviyeleri yükseldi. Demir birikimini göstermek için kullanılan Prusya mavisi (serbest demir lekesi) boyaması, N-asetil sistein grubunda demir birikiminin azaldığını ortaya çıkarmıştır.
Sonuç: Bu çalışmanın sonuçunda, bir sıçan modelinde alt ekstremite iskemisi-reperfüzyonunun neden olduğu ferroptozise bağlı akciğer hasarı üzerine N-asetil sisteinin koruyucu bir etkisi olduğunu düşündürmektedir.

Keywords

Feroptozis, İskemi/ reperfüzyon, n-asetil sistein

Supporting Institution

YOKTUR

N-acetyl cysteine attenuates ferroptosis mediated lung injury induced by lower limb ischaemia/reperfusion

Abstract

Aim: This study aimed to analyze the effect of N-acetyl cysteine pretreatment on the prevention of ferroptosis mediated lung injury induced by lower limb ischemia and reperfusion.
Material and Methods: Eighteen male Sprague-Dawley rats weighing 350-400 g were randomized into three groups. The animals received N-acetyl cysteine 150 mg/kg or normal saline 0.1 ml/kg intraperitoneally before the ischemic period. In the control and study groups, I/R injury was induced by clamping the aorta infrarenal for 2 hours, followed by 4 hours of reperfusion. The third group underwent sham surgery. After sacrifice, the lungs of the animals were extracted for both histopathological and biochemical analysis.
Results: There was a significant difference between the control and study animals regarding tissue malondialdehyde (MDA), and glutathione (GSH) levels. In the control group, the MDA levels were increased and the GSH levels were increased significantly compared to the sham group that revealed a ferroptosis mediated lung injury. However, N-acetyl cysteine decreased the levels of MDA and increased the levels of GSH revealing a protective effect. The Prussian blue (free iron stain) staining which was used to examine iron deposition revealed a reduced deposition of iron in the N-acetyl cysteine group.
Conclusion: The results of the present study suggest a protective effect of N-acetyl cysteine on ferroptosis mediated lung injury induced by lower limb ischemia-reperfusion in a rat model.

Keywords

ferroptosis, N-ACETYL CYSTEINE, ischemia/reperfusion

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