THE EFFECT OF INOCULATION OF BRADYRHIZOBIUM SP. LUPINUS ON PLANT DEVELOPMENT AND YIELDING OF NARROW-LEAVED LUPIN

Abstract

A field experiment was conducted in the Wielkopolska region at the Gorzyń Research Station, Poland (52.34°N, 15.54°E) in Central Europe. The study was conducted over a 3-year period (2017, 2018, 2019) as a two-factorial desingn with four replications in the randomised plots. The aim of the research was to determine the effect of the cultivar (‘Bolero’, ‘Tytan’) and the inoculation (Nitragina–seeds inoculation, Nitroflora I–seeds inoculation, Nitroflora II–soil inoculation, HiStick® Lupin–seeds inoculation) on plant development, seeds chemical composition and yielding of narrow-leaved lupin. The weather conditions and experimental factors significantly influenced on productivity of narrow-leaved lupin ‘Tytan’. Drought during the growing season reduced seeds and protein yields. After inoculation of HiStck the seeds yield was significantly greater by 12.4% and the protein yield after application of Nitroflora I or HiStick by 13.9% and 19.2%, respectively. Correlation coefficients showed strong relations between number of pods and seeds per plant in both cultivars regardless of the inoculation variant, however the strongest relations in both cultivar were proved on HiStick treatment.

Keywords

• Biological nitrogen fixation
• chemical composition
• legumes
• protein efficiency
• yielding

Supporting Institution

Poznań University of Life Sciences

Thanks

We gratefully thank Professor Jerzy Szukała (Department of Agronomy, Faculty of Agronomy and Bioengineering, Poznań University of Life Sciences) for the opportunity to carry out this experiment within the project Polish Ministry of Agriculture and Rural Development (Project: No. HOR 3.1/2016–2020) and for supporting research on cultivation of legumes.

References

1. Annicchiarico, P.N., Harzic and A.M. Carroni. 2010. Adaptation, diversity, and exploitation of global white lupin (Lupinus albus L.) landrace genetic resources. Field Crop Res. 119: 114-124. https://doi.org/10.1016/j.fcr.2010.06.022.
2. AOAC. 2011. Official Methods of Analysis of AOAC International, 18th ed. Horwitz W, Latimer GW Jr, Eds. Revision 4. AOAC International, Gaithersburg, MD, USA.
3. Atkins, C.A. and P.M. Smith. 2004. Regulation of pod set and seed development in lupin. In proceedings of the regulation of pod set and seed development in lupin. Laugarvatn, Iceland.
4. Campo, R.J., R.S. Araujo and M. Hungria. 2009. Molybdenumenriched soybean seeds enhance N accumulation, seed yield, and seed protein content in Brazil. Field Crops Res. 110: 219- 224.
5. de Borja Reis, A.F., L.M. Rosso, L.C. Purcell, S. Naeve, S.N. Casteel, P. Kovács, S. Archontoulis, D. Davidson and I.A. Ciampitti. 2021. Environmental factors associated with nitrogen fixation prediction in soybean. Front. Plant Sci. 12: 1013.
6. de Visser, C.L.M., S. Remco and F. Stoddard. 2014. The EU’s dependency on soya bean import for the animal feed industry and potential for EU produced alternatives. OCL 21: D407. https://doi.org/10.1051/ocl/2014021.
7. Erbas, M., M. Certel and M.K. Uslu. 2005. Some chemical properties of white lupin seeds (Lupinus albus L.). Food Chem. 89: 341-345.
8. Etemadi, F., M. Hashemi, A.V. Barker, O.R. Zandvakili and X. Liu. 2016. Agronomy, nutritional value, and medicinal application of faba bean (Vicia faba L.). Horticult. Plant J. 5: 170-182. https://doi.org/10.1016/j.hpj.2019.04.004.

9. Faligowska, A., K. Panasiewicz, G. Szymańska, J. Szukała, W. Koziara and A. Pszczółkowska. 2017. Productivity of white lupin (Lupinus albus L.) as an effect of diversified farming systems. Legume Res. 40: 872-877. DOI: 10.18805/lr.v0i0.8400.
10. Faluyi, M.A., X.M. Zhou, F. Zhang, S. Leibovitch, P. Migner and D.L. Smith. 2000. Seed quality of sweet white lupin (Lupinus albus) and management practice in eastern Canada. Europ. J. Agronomy 13: 27-37.
11. FAOSTAT. 2022. http://www.fao.org/faostat/en/#data/QCL.
12. Hungria, M. and M.A.T. Vargas. 2000. Environmental factors affecting N2 fixation in grain legumes in the tropics, with an emphasis on Brazil. Field Crops Res. 65: 151-164.
13. Kalembasa, D., J. Szukała, B. Symanowicz, S. Kalembasa, A. Faligowska and M. Becher. 2021. Amount of biologically nitrogen fixed by faba bean and its uptake by winter wheat determined by 15N ID method. Arch. Agron. Soil Sci. 67: 1875-1888. https://doi.org/10.1080/03650340.2020.1817398.
14. Księżak, J. and J. Bojarszczuk. 2022. The effect of mineral N fertilization and Bradyrhizobium japonicum seed inoculation on productivity of soybean (Glycine max (L.) Merrill). Agriculture 12: 110. https://doi.org/10.3390/agriculture12010110.
15. Księżak, J., M. Staniak and J. Bojarszczuk. 2009. The regional differentiation of legumes cropping area in Poland between 2001 and 2007. Pol. J. Agron. 1: 25-31. https://doi.org/10.26114/pja.iung.016.2009.01.05.
16. Lucas, M.M., F.L. Stoddard, P. Annicchiarico, J. Frias, C. Martinez-Villaluenga and D. Sussmann. 2015. The future of lupin as a protein crop in Europe. Front. Plant Sci. 6: 705. https://doi.org/10.3389/fpls.2015.00705.
17. Lumactud, R.A., D. Dollete, D.K. Liyanage, K. Szczyglowski, B. Hill and M.S. Thilakarathna. 2023. The effect of drought stress on nodulation, plant growth, and nitrogen fixation in soybean during early plant growth. J. Agron. Crop Sci. 209: 345-354. https://doi.org/10.1111/jac.12627.
18. Lybæk, R. and H. Hauggaard-Nielsen. 2019. The use of faba-bean cropping as a sustainable and energy saving technology – A new protein self-sufficiency opportunity for European agriculture? IOP Conf. S. Earth Environ. Sci. 291: 012049. https://doi.org/10.1088/1755-1315/291/1/012049
19. Murtaza, G., Ehsanullah, A. Zohaib, S. Hussain, T. Rasool, H. Shehzad. 2014. The influence of rhizobium seed inoculation and different levels of phosphorus application on growth, yield and quality of mashbean (Vigra mungo L.). Int. J. Mod. Agric. 3: 92-96. https://doi.org/10.17762/ijma.v3i3.47.
20. Mut, H., A. Gulumser, I. Ayan, Z. Acar, U. Basaran and O. OnalAsci. 2012. Effects of cultivar, inoculation, and sowing date in seed yield and yield components of lupin. J. Plant Nutr. 35: 1290-1302. DOI: 10.1080/01904167.2012.684122.
21. Ouma, E.W., A.M. Asango, J. Maingi and E.M. Njeru. 2016. Elucidating the potential of native rhizobial isolates to improve biological nitrogen fixation and growth of common bean and soybean in smallholder farming systems of Kenya. Int. J. Agronomy 4569241: 1-7. http://dx.doi.org/10.1155/2016/4569241.
22. Ozturk, G. and Z. Yildirim. 2013. Effect of bio-activators on the tuber yield and tuber size of potatoes. Turkish Journal of Field Crops 18(1): 82-86.
23. Panasiewicz, K. 2022. Chemical composition of lupin (Lupinus spp.) as influenced by variety and tillage system. Agriculture 12: 263. https://doi.org/10.3390/agriculture12020263.
24. PN-68/R-74017. 1969. Cereal grains and pulses. Determination of the weight of one thousand grains. Guideline (Polish).
25. Preissel, S., M. Reckling, N. Schläfke and P. Zander. 2015. Magnitude and farm-economic value of grain pre-crop benefits in Europe. A Review. Field Crops Res. 175: 64-79. https://doi.org/10.1016/j.fcr.2015.01.012.
26. Prusiński, J., A. Baturo-Cieśniewska and M. Borowska. 2020. Response of soybean (Glycine max (L.) Merrill) to mineral nitrogen fertilization and Bradyrhizobium japonicum seed inoculation. Agronomy 10: 1300. https://doi.org/10.3390/agronomy10091300.
27. Pudełko, K. and J. Żarnicka. 2010. Diversity in symbiotic specificity of bacterial strains nodulating lupins in Poland. Pol. J. Agron. 2: 50-56.
28. Samudin, S. and H. Kuswantoro. 2018. Effect of Rhizobium inoculation to nodulation and growth of soybean [Glycine max (L.) Merrill] germplasm. Legume Res. 41: 303-310. DOI: 10.18805/LR-385.
29. Singh, A.K., R.C. Bharati, N.C. Manibhushan and A. Pedpati. 2013. An assessment of faba bean (Vicia faba L.) current status and future prospect. African J. Agricult. 8: 6634-6641. https://doi.org/10.5897/AJAR2013.7335.
30. Stein, H.H., L.L. Berger, J.K. Drackley, G.C. Jr Fahey, D.C. Hernot and C.M. Parsons. 2008. 18- Nutritional properties and feeding values of soybeans and their co-products. In Soybeans. Chemistry Production Processing and Utilization.
31. Johnson LA, White PJ, Galloway R, Eds. AOCS Press, Urbana, IL, USA. https://doi.org/10.1016/B978-1-893997- 64-6.50021-4.
32. Steel, R.G.D. and J.H. Torrie. 1980. Principles and Procedures of Statistics. A Biometrical Approach. 2nd edition. McGrawHill, New York, USA, pp. 20-90.
33. Sujak, A., A. Kotlarz and W. Strobel. 2006. Compositional and nutritional evaluation of several lupin seeds. Food Chem. 98: 711-719. https://doi.org/10.1016/j.foodchem.2005.06.036.
34. Sulewska, H., K. Ratajczak, A. Niewiadomska and K. Panasiewicz. 2019. The use of microorganisms as biofertilizers in the cultivation of white lupine. Open Chem. 17: 813-822. https://doi.org/10.1515/chem-2019-0089.
35. Szpunar-Krok, E. and A. Wondołowska-Grabowska. 2022. Quality evaluation indices for soybean oil in relation tocultivar, application of N fertiliser and seed inoculation with Bradyrhizobium japonicum. Foods 11: 762. https://doi.org/10.3390/foods11050762.
36. Szpunar-Krok, E., A. Wondołowska-Grabowska, D. BobreckaJamro, M. Jańczak-Pieniążek, A. Kotecki and M. Kozak. 2021. Effect of nitrogen fertilization and inoculation with Bradyrhizobium japonicum on the fatty acid profile of soybean (Glycine max (L.) Merrill) seeds. Agronomy 11: 941. https://doi.org/10.3390/agronomy11050941.
37. Thrane, M., P.V. Paulsen, M.V. Orcutt and T.M. Krieger. 2017. Soy protein: Impacts. production. and applications (Ch. 2). In Sustainable Protein Sources. Nadathu SR, Wanasundara JPD, Scanlin L, Eds. Academic Press, Cambridge, MA, USA. https://doi.org/10.1016/B978-0-12-802778-3.00002-0.
38. Ton, A., T. Karakoy, A.E. Anlarsal and M. Turkeri. 2021. Genetic diversity for agro-morphological characters and nutritional compositions of some local faba bean (Vicia faba L.) genotypes. Turk. J. Agric. For. 45(3): 6. https://doi.org/10.3906/tar-2008-74.
39. Tounsi-Hammami, S., S. Dhane-Fitouri, Ch. Le Roux, Z. Hammami and F.B. Jeddi. 2020. Potential of native inoculum to improve the nodulation and growth of white lupin in Tunisia. Annales de l’INRA 93: 102-114.
40. Tsukamoto, C.H., M.A. Nawaz, A. Kurosaka, B. Le, J.D. Lee, E. Son, S.H. Yang, C. Kurt, F.S. Baloch and G. Chung. 2018. Isoflavone profile diversity in Korean wild soybeans (Glycine soja Sieb. & Zucc.). Turk. J. Agric. For. 42(4): 3. https://doi.org/10.3906/tar-1801-95.
41. Van Soest, P.J. 1963. Use of detergents in the analysis of fibrous feeds. II. A rapid method of determination of fibre and lignin. Journal of AOAC International 46: 829-835. https://doi.org/10.1093/jaoac%2F46.5.829.
42. Yousaf, S., A. Zohaib, S.A. Anjum, T. Tabassum, T. Abbas, S. Irshad, U. Javed and N. Farooq. 2018. Effect of seed inoculation with plant growth promoting rhizobacteria on yield and quality of soybean. Pak. J. Agric. Res. 32: 177-184. http://dx.doi.org/10.17582/journal.pjar/2019/32.1.177.184.
43. Zetochová, E., A. Vollmannová and I. Tirdiľová. 2020. Effect of inoculation on the content of biogenic elements in the white lupine and grass pea. Potravinarstvo Slovak J. Food Sci. 14: 385-392. https://doi.org/10.5219/1327.
44. Zveushe, O.K., V.R. de Dios, H. Zhang, F. Zeng, S. Liu, S. Shen, Q. Kang, Y. Zhang, M. Huang, A. Sarfaraz, M. Prajapati, L. Zhou, W. Zhang, Y. Han and F. Dong. 2023. Effects of CoInoculating Saccharomyces spp. with Bradyrhizobium japonicum on Atmospheric Nitrogen Fixation in Soybeans (Glycine max (L.). Plants 12: 681. https://doi.org/10.3390/plants12030681.